177,604 results
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Plasmid#60500PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only
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pSBtet-RB
Plasmid#60506PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
single polypeptide FPX biosensor for caspase-1
Plasmid#60885PurposeFPX biosensor for caspase-1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsingle polypeptide FPX biosensor for caspase-1
TagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-N1
Plasmid#58187DepositorTypeEmpty backboneTagsSNAP tagExpressionMammalianAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pLKO.DEST.puro
Plasmid#32682PurposeLentiviral cDNA/shRNA gateway destination vectorsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceDec. 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VV229: GCaMP6f in fck
Plasmid#58514PurposeExpresses GCaMP6f under the a-CamKII promoterDepositorInsertGCaMP6f
UseLentiviralTagsC-terminal FCYENEV (ER export motif); this was no…ExpressionMammalianPromotera-CamKIIAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-VASP-5
Plasmid#55151PurposeLocalization: Actin/Focal Adhesions, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVASP
TagsmCherryExpressionMammalianMutationA209T in VASPPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFasBac+GFP-CAMSAP2
Plasmid#59037Purposefor baculovirus expression of CAMSAP2 in Sf9 cellsDepositorAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FRP467-PACT1(-1-520)-LexA-ER-haVP16
Plasmid#58430PurposeExpresses LexA-ER-VP16 in yeast cellsDepositorInsertLexA-ER-VP16
ExpressionYeastPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-Vac 14 Full Length
Plasmid#47418DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVac14 (VAC14 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Dronpa3-C1
Plasmid#54655PurposeLocalization: C1 Cloning Vector, Excitation: 503, Emission: 518DepositorTypeEmpty backboneTagsDronpa3ExpressionMammalianAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGreen_dualluc_ORF_sensor
Plasmid#55207PurposeDual-luciferase reporter for microRNA targeting in plantsDepositorInsertsRenilla luciferase
Firefly luciferase
UseLuciferase; Plant transient expressionAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mEos4b-Lifeact-7
Plasmid#54813PurposeLocalization: Actin, Excitation: 505 / 569, Emission: 516 / 581DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifeact-mEos4b
ExpressionMammalianAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
IFP2.0-C1
Plasmid#54784PurposeLocalization: C1 Cloning Vector, Excitation: 684, Emission: 708DepositorTypeEmpty backboneTagsIFP2.0ExpressionMammalianAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D
Plasmid#52521PurposeCo-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virusDepositorInsertChR2-2a-hM4D
UseAAV and Cre/LoxTags2xHA on ChR2PromoterCBAAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-hGPR56-IRES-GFP
Plasmid#52297PurposeExpresses human GPR56 and GFP in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuman GPR56 coding sequence (ADGRG1 Human)
ExpressionMammalianPromoterCAG promoterAvailable sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50470PurposeGq-coupled hM3D-IRES-mCitrine under the control of GFAP promoterDepositorInserthM3D(Gq)-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCRISPR - EGFP sgRNA 3
Plasmid#51762PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 3
UseCRISPR and LentiviralExpressionMammalianPromoterEFS and U6Available sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-p65AD-GBP6
Plasmid#49440PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGBP6-p65 transactivation domain fusion protein
ExpressionMammalianPromoterCAGAvailable sinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-GBP1-10gly-Gal4DBD
Plasmid#49438PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGBP1-Gal4 DNA binding domain fusion protein
ExpressionMammalianMutationC98S (to increase protein solubility), Q3DPromoterCAGAvailable sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by