174,421 results

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GFP-RAB10

Plasmid

#130883

Purpose

Expresses human RAB10 with N-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAR-Ec318

Plasmid

#130871

Purpose

A nuclear plasmid encoding the WT TP-DNAP1 for OrthoRep

Depositor

Chang Liu

Article

Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

Insert

TP-DNAP1

Expression

Yeast

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-H2B-iRFP720

Plasmid

#128961

Purpose

Lentiviral plasmid for expression of far red iRFP720 nuclear marker

Depositor

Carlos Carmona-Fontaine

Insert

Human histone H2B (H2BC21 Human)

Use

Lentiviral

Tags

iRFP720

Expression

Mammalian

Promoter

hPGK

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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TOM20*-SBP-GFP

Plasmid

#120173

Purpose

Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Mitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)

Tags

SBP and eGFP

Expression

Mammalian

Mutation

Truncated TOM20: 1-30 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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LV-Sox10MCS5-GFP

Plasmid

#115783

Purpose

This plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.

Depositor

Fraser Sim

Article

Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

Insert

mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter

Use

Lentiviral

Tags

cfos basal promoter conjugated MCS5 promoter fuse…

Promoter

SOX10 Multiple Species Conserved enhancer element…

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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LRG2.1_Puro

Plasmid

#125594

Purpose

Lentiviral expression of sgRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pESF140_HisSpy_DDB1∆B

Plasmid

#124213

Purpose

Insect cell expression vector for His6, Spy tagged hsDDB1∆B

Depositor

Eric Fischer

Article

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

DNA damage-binding protein 1 (DDB1 Human)

Tags

His6-Spy

Expression

Insect

Mutation

internal deletion of BPB domain to aid crystalliz…

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pZ149-pcDNA3-BhCas12b_v4

Plasmid

#122446

Purpose

Expresses BhCas12b_v4 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BhCas12b_v4-NLS-3xHA

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K27R

Plasmid

#121155

Purpose

Mammalian expression of HA tagged ubiquitin K27R

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

K27 mutated to an R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMK346 (Hygro-P2A-mAID-mCherry)

Plasmid

#121180

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID-mCherry

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P05

Plasmid

#120016

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-Ost1-alphaf(I)-E2-Crimson

Plasmid

#117661

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-alphaf(I)-E2-Crimson

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-GFP11-pDEST-N

Plasmid

#118366

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-Flag

Expression

Mammalian

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-dLight1.1

Plasmid

#111067

Purpose

To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1 and AAV5

Insert

dLight1.1

Use

AAV

Tags

Flag tag

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only
pScI_dCas9-CDA_J23119-sgRNA

Plasmid

#108550

Purpose

Bacterial Target-AID vector with sgRNA

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Inserts

dCas9-PmCDA1
Streptococcus pyogenes sgRNA

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

J23119 and lambda OR

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL-TetO-Pax7-IRES-EGFP

Plasmid

#109372

Purpose

Expresses Dox-inducible Pax7 together with a GFP reporter

Depositor

Nenad Bursac

Article

Rao et al Nat Commun. 2018 Jan 9;9(1):126. doi: 10

Insert

Pax7

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti NL

Plasmid

#113450

Purpose

Lentiviral NanoLuc control expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

NanoLuc

Use

Lentiviral and Luciferase

Tags

cmyc

Expression

Mammalian

Promoter

hUbC

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pIGAhRC

Plasmid

#112510

Purpose

human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoter

Depositor

Charles Miller

Article

Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

Insert

HIF1B, AhR (ARNT, AHR Budding Yeast, Human)

Use

Synthetic Biology

Expression

Yeast

Promoter

Gal1,10

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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PtPuc3_diaCas9_sgRNA

Plasmid

#109219

Purpose

Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

Depositor

Per Winge

Article

Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

Inserts

LHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region

Use

CRISPR and Synthetic Biology

Promoter

LHCF1 terminator, LHCF2 promoter, U6 3' regi…

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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FUS-Citrine-PixE

Plasmid

#111505

Purpose

For PixELL system

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

PixE (slr1693 )

Use

Lentiviral

Tags

Citrine and FUS

Promoter

SFFV

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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