We narrowed to 22,456 results for: his
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Plasmid#85734PurposeExpresses DNA-binding (ETS) domain of murine PU.1, residues 167-272DepositorInsertETS domain of murine PU.1 (residues 167-272) (Spi1 Mouse, Human)
Tags6xHis following thrombin cleavage site LVPR|GExpressionBacterialAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLacI241-Wasabi
Plasmid#86102PurposemWasabi reporter gene gated by temperature-sensitive LacI A241TDepositorInsertsLacIts
mWasabi
Tags6xHisExpressionBacterialMutationA241TPromoterT7A1O4/O3 and pLacIAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEN6-Ubc5 Sc
Plasmid#83234PurposepHis-par2-UbHs/Ubc5Sc/ Uba1TaDepositorAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRAF-MutH
Plasmid#86609PurposeE. coli MutH expression plasmidDepositorInsertE. coli MutH
Tagshis-tag and ald-tagExpressionBacterialPromoterT7Available SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)-SPN+IFS
Plasmid#83372PurposeExpresses SPN and IFS complex in E. coli cellDepositorInsertsSPN
IFS
TagsHexahistidine tagExpressionBacterialPromoterT7Available SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3R112C/T180CBM3
Plasmid#85090PurposeExpresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coliDepositorInsertThe R112C/T180C variant of PCNA3 fused to P450 BM3
TagsHis6 tagExpressionBacterialMutationR112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3PromoterT7 promoterAvailable SinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-SUMO-KaiB
Plasmid#68050PurposeStrain: AM5116, Used for protein purificationDepositorInsertKaiB
Tags6xHis and SUMOExpressionBacterialPromoterT7Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SRAP-delCys2
Plasmid#84568PurposeEncodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2DepositorInsertSrap1 (Hmces Mouse)
Tags6xHis and S-TagExpressionBacterialMutationdeletion of Cys2PromoterT7Available SinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + PrgJ
Plasmid#84869PurposeBaculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
PrgJ
UseBaculovirusTags6xHisExpressionInsectPromoterPolyhedrinAvailable SinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
rFIXX-bp chain A (IRESneo)
Plasmid#82450PurposeChain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.DepositorInsertrFIXX-bp chain A
TagsPolyhistidineExpressionBacterial and MammalianPromoterT7 RNA polymerase promoterAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO CD16.7-h-PKD1 FLM R4114A
Plasmid#83461PurposeCytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)DepositorInsertPolycystin-1 (PKD1 Human)
TagsCD16.7ExpressionMammalianMutationAmino-acids 4078-4303, R4113AAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-PKD1 FLS (4107-4303)-GST
Plasmid#83456PurposeHuman PC1-CTp30 expression cassette with a GST-tag (678)DepositorAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(deltaP)
Plasmid#83507PurposeBacterial expression of Calreticulin mutantDepositorInsertCALR (Calr Mouse)
TagsHisExpressionBacterialMutationdelta . Deletion of residues 187-283PromoterT7Available SinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(deltaC)
Plasmid#83506PurposeBacterial expression of Calreticulin mutantDepositorInsertCALR (Calr Mouse)
TagsHisExpressionBacterialMutationdelta C. Contains residues 1-339PromoterT7Available SinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE30-roGFP1-iE
Plasmid#83312PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-iE
Tags6xHISExpressionBacterialMutationro1(C48S/S147C/Q204C) plus X insertion, iE betwe…PromoterT5Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-160-GCN4
Plasmid#74608PurposeExpresses artificially dimerized Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
Tags6x His, GCN4, Strep II, and superfolder GFPExpressionBacterialAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_4
Plasmid#78208PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak bindingDepositorInsertmatB (His6 C106A R286C T303A Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A R286C T303A Q457C K488APromoterT7Available SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_3
Plasmid#78207PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak bindingDepositorInsertmatB (His6 C106A T167A R286C Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A T167A R286C Q457C K488APromoterT7Available SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJKW 0643
Plasmid#74121PurposePhotinus pyralis luciferin sulfotransferase LST - pHis8-4DepositorInsertPhotinus pyralis firefly luciferin sulfotransferase
Tags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialPromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
GALTA-c001
Plasmid#79645PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only