4,299 results

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pT7-hMLH1dn for IVT

Plasmid

#178114

Purpose

Template for in vitro transcription of human MLH1dn

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (codon optimized) (MLH1 Human)

Use

Template for in vitro transcription

Mutation

Detailed in manuscript

Promoter

T7 (inactivated)

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-FLAG-hSF3B1-K700E

Plasmid

#82577

Purpose

Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

Depositor

Manoj Pillai

Article

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-K700E (SF3B1 Human)

Tags

FLAG peptide

Expression

Mammalian

Mutation

K700E

Promoter

CMV

Available Since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-hSynI-V5-TurboID(N)-GPI

Plasmid

#163696

Purpose

Expresses n-terminal half of TurboID on surface of neurons

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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ilux pGEX(-)

Plasmid

#107879

Purpose

Bacterial expression of ilux for bioluminescence imaging

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

Insert

ilux

Expression

Bacterial

Promoter

tac

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2 (GFP)

Plasmid

#80924

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pY002 (pFnCpf1_min)

Plasmid

#69975

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes nd artificial promoter

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pOPTN-EGFP

Plasmid

#27052

Depositor

Beatrice Yue

Article

Park et al Am J Pathol. 2006 Dec . 169(6):1976-89.

Insert

optineurin (OPTN Human)

Expression

Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pBPGUw

Plasmid

#17575

Depositor

Gerald Rubin

Article

Pfeiffer et al Tools for neuroanatomy and neurogenetics

Insert

GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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3xnls-mNeonGreen

Plasmid

#98875

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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GW1-pHRed

Plasmid

#31473

Purpose

genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

Depositor

Gary Yellen

Article

Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

Insert

pHRed

Expression

Mammalian

Available Since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

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EGFP-alphasynuclein-WT

Plasmid

#40822

Depositor

David Rubinsztein

Article

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pLX313-Renilla luciferase

Plasmid

#118016

Purpose

Constitutive expression of Renilla luciferase

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Renilla luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 R175H

Plasmid

#16436

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R175H (TP53 Human)

Expression

Mammalian

Mutation

R175H

Promoter

CMV

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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hAAVS1 1L TALEN

Plasmid

#35431

Purpose

TALEN designed to target the human AAVS1 locus

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

hAAVS1 1L TALEN

Expression

Mammalian

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only

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lenti sgRNA(MS2)_zeo backbone

Plasmid

#61427

Purpose

3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLL7.0: Venus-iLID-CAAX (from KRas4B)

Plasmid

#60411

Purpose

Mammalian Expression of Venus-iLID-CAAX

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-iLID-CAAX

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCRII_TOPO_hNEAT1

Plasmid

#61518

Purpose

human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

Depositor

Archa Fox

Article

Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

Insert

NEAT1 (NEAT1 Human)

Use

General cloning vector

Mutation

*see comment below

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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mEGFP-NbALFA

Plasmid

#159986

Purpose

For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mEGFP-NbALFA

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mEGFP and NbALFA

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hM3D(Gq)-mCherry

Plasmid

#50476

Purpose

Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter

Depositor

Bryan Roth

Has Service

AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

hM3D(Gq)-mCherry (CHRM3 Human)

Use

AAV

Tags

mCherry

Mutation

See supplemental documents for DREADD mutations

Promoter

CaMKIIa

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV mGAD65(delE1) GFP WPRE SV40pA

Plasmid

#177316

Purpose

To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

Depositor

Hirokazu Hirai

Article

Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

Insert

mGAD65(delE1) promoter, GABAergic interneurons specific promoter

Use

AAV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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