Addgene

We narrowed to 151,085 results for: Des

Showing: 2201 - 2240 of 151085 results

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CamKII-LGI1-E383A-pHluorin

Plasmid

#185544

Purpose

Expresses mutant E383A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed glutamic acid 383 for alanine

Promoter

CamKII

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-C200R-pHluorin

Plasmid

#185545

Purpose

Expresses mutant C200R LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed cysteine 383 for arginine

Promoter

CamKII

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-IgK-pHluorin-TM-mRuby

Plasmid

#185546

Purpose

Expresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

IgK-pHluorin-TM-mRuby

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSF-pMB1'- BAD-YFP

Plasmid

#186567

Purpose

pMB1' ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

BAD promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSF-p15A-T7- YFP

Plasmid

#186568

Purpose

p15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSF-pMB1'- trc-YFP

Plasmid

#186565

Purpose

pMB1' ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSF-pMB1'- tac-YFP

Plasmid

#186566

Purpose

pMB1' ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGL2-hOct4 DE-SV40-Luc

Plasmid

#26279

Purpose

Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Insert

Oct4 Distal Enhancer Element (DE) (POU5F1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

See Depositor Comments below

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pDSM-de-Pfba1-PYC-Trpl15a

Plasmid

#127738

Purpose

Yeast pathway position 5. PYC transcription unit with the FBA1 promoter and RPL15A terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pfba1

Available since

Jan. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL(T56A,DE)

Plasmid

#25059

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56A,DE) (BCL2L11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to alanine changed leucine …

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(T56D,DE)

Plasmid

#25060

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56D,DE) (BCL2L11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to aspartic acid changed le…

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pGG-GOI-DE-TurboID-GFP

Plasmid

#209389

Purpose

Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Tags

GFP

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDSM-de-Phhf2-PYC-Tadh1

Plasmid

#127737

Purpose

Yeast pathway position 5. PYC transcription unit with the HHF2 promoter and ADH1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Phhf2

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 BimL(S58D,DE)

Plasmid

#25057

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58D,DE) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to aspartic acid changed leuci…

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(S44A,DE)

Plasmid

#25054

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44A,DE) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed serine 44 to alanine changed leucine 92 …

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(T56A,DE)

Plasmid

#25055

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56A,DE) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed threonine 56 to alanine changed leucine …

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL(S58A,DE)

Plasmid

#25056

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58A,DE) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to alanine changed leucine 92 …

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(S44D,DE)

Plasmid

#25058

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44D,DE) (BCL2L11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 44 to aspartic acid changed leuci…

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(S58D,DE)

Plasmid

#25061

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58D,DE) (BCL2L11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 58 to aspartic acid changed leuci…

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pDSM-de-Ppgk1-PYC-Ttpi1

Plasmid

#127735

Purpose

Yeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Ppgk1

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pDSM-de-Ppxr1-PYC-Tnat1

Plasmid

#127736

Purpose

Yeast pathway position 5. PYC transcription unit with the PXR1 promoter and NAT1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Ppxr1

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-human OCT4 DE-SV40-Luc

Plasmid

#52414

Purpose

Luciferase reporter plasmid for DE OCT4 enhancer activity

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Distal element of human OCT4 promoter (POU5F1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCD1C

Plasmid

#102954

Purpose

gene cloning of human CD1C

Depositor

Gennaro De Libero, Lucia Mori

Article

Lepore et al J Exp Med. 2014 Jun 30;211(7):1363-77. d

Insert

CD1C cDNA (CD1C Human)

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pHMR1A

Plasmid

#102957

Purpose

gene cloning of MR1A

Depositor

Gennaro De Libero, Lucia Mori

Article

Lepore et al Nat Commun. 2014 May 15;5:3866. doi: 10.

Insert

MR1A cDNA (MR1 Human)

Use

Cloning

Tags

Expression

Mutation

Stop codon has been removed by HindIII site

Promoter

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCD1A

Plasmid

#102952

Purpose

gene cloning of human CD1A

Depositor

Gennaro De Libero, Lucia Mori

Article

Manolova et al Eur J Immunol. 2003 Jan;33(1):29-37.

Insert

CD1A cDNA (CD1A Human)

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-EGFP-Hu_Plk1(WT)

Plasmid

#136323

Purpose

Retroviral expression of human Plk1-WT with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Insert

PLK1 (PLK1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPC Flag-mRAP1

Plasmid

#73135

Purpose

Expression of Flag-tagged mRAP1

Depositor

Agnel Sfeir, Titia de Lange

Article

Sfeir et al Science. 2010 Mar 26;327(5973):1657-61.

Insert

Rap1 (Terf2ip Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCD1B

Plasmid

#102953

Purpose

mammalian expression of CD1B

Depositor

Gennaro De Libero, Lucia Mori

Article

Manolova et al Eur J Immunol. 2003 Jan;33(1):29-37.

Insert

CD1B cDNA (CD1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR_Mm_CEP55(WT)

Plasmid

#136333

Purpose

Mouse CEP55 cDNA in Gateway pENTR

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Commun. 2018 Aug 1;9(1):3012. doi: 1

Insert

CEP55 (Cep55 Mouse)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI_Hu_ECT2(WT)

Plasmid

#136330

Purpose

Lentiviral expression of human ECT2 WT

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ECT2 (ECT2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Hu_Plk5(WT)

Plasmid

#136319

Purpose

Mammalian expression of human Plk5-WT with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (PLK5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCD1Dsol

Plasmid

#102955

Purpose

Mammalian expression of human soluble CD1D cDNA

Depositor

Gennaro De Libero, Lucia Mori

Article

Facciotti et al Proc Natl Acad Sci U S A. 2011 Aug 23;10

Insert

Human soluble CD1D cDNA (CD1D Human)

Use

Tags

BirA

Expression

Mammalian

Mutation

Lacks the transmembrane region

Promoter

CMV

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPC myc-mRAP1

Plasmid

#73136

Purpose

Expression of myc-tagged mRAP1

Depositor

Agnel Sfeir, Titia de Lange

Article

Sfeir et al Science. 2010 Mar 26;327(5973):1657-61.

Insert

Rap1 (Terf2ip Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR_Hu_Plk5(WT)

Plasmid

#136329

Purpose

Human Plk5 WT in Gateway pENTR

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (PLK5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Mm_MyoGEF(WT)

Plasmid

#136325

Purpose

Mammalian expression of mouse MyoGEF-WT with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

PLEKHG6 (Plekhg6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI_Hu_ECT2(T327D)

Plasmid

#136332

Purpose

Lentiviral expression of human ECT2_T327D

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ECT2 (ECT2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

T327D

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Mm_Plk5(300-595) PBD domain

Plasmid

#136316

Purpose

Mammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (Plk5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

fragment 300-595

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Mm_Plk5(D168A)

Plasmid

#136318

Purpose

Mammalian expression of mouse Plk5-KD (D168A) with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (Plk5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

D168A

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Hu_Plk5(W207F)

Plasmid

#136320

Purpose

Mammalian expression of human Plk5 W207F with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (PLK5 Human)

Use

Tags

Expression

Mammalian

Mutation

W207F

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-Mm_MyoGEF(WT)

Plasmid

#136327

Purpose

Mouse MyoGEF-WT in Gateway pENTR

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

PLEKHG6 (Plekhg6 Mouse)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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