We narrowed to 5,797 results for: plasmid dna

1. Bxb1-GA_CAG-TurboGFP

   Plasmid

   #229781

   Purpose

   Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TurboGFP
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AX53_pS.Donor.S1.TS

   Plasmid

   #100290

   Purpose

   AAVS1-targeting plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   18 bp RFLP (MluI+KspAI)
   

   Use

   CRISPR and Synthetic Biology; Human targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLPC31

   Plasmid

   #209996

   Purpose

   Contains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   3' Connector (3C2SN)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLPC32

   Plasmid

   #209997

   Purpose

   Contains Level 0 Part: 3' Connector (3C5CSN) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   3' Connector (3C5CSN)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLPC52

   Plasmid

   #209991

   Purpose

   Contains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   5' Connector (5C2SN)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLPC51

   Plasmid

   #209990

   Purpose

   Contains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   5' Connector (5C1CSN)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPBT-peRNA_GG-Puro

   Plasmid

   #173220

   Purpose

   piggyBac transposon containing hU6 promotor and pegRNA cloning site

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

   Inserts

   pegRNA cloning cassette
   PuroR
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and hU6

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKB01-PssbB-mKate2

   Plasmid

   #69105

   Purpose

   Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   mKate2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AV09_pDonor.EP.S1.TS

   Plasmid

   #100297

   Purpose

   AAVS1-targeting expression plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   PuroR.T2A.EGFP expression unit
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLuciferase (GB0096)

    Plasmid

    #68201

    Purpose

    Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luciferase coding region
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AY42_pS.Donor.R5

    Plasmid

    #100291

    Purpose

    CCR5-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    18 bp RFLP (MluI+KspAI)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAF256

    Plasmid

    #105496

    Purpose

    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and LgBiT

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

    Insert

    HupA-SmBiT/LgBiT
    

    Use

    Atc-dependent expression in c. difficile

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAF257

    Plasmid

    #105497

    Purpose

    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible SmBiT and HupA-LgBiT

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

    Insert

    SmBiT/HupA-LgBiT
    

    Use

    Atc-dependent expression in c. difficile

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRenilla (GB0056)

    Plasmid

    #68198

    Purpose

    Provides the CDS of the Renilla protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Renilla coding region
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FFiBlast MSCV Puro

    Plasmid

    #68464

    Purpose

    Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Blasticidin Resistance
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Inverted

    Promoter

    LTR

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA0055

    Plasmid

    #131774

    Purpose

    Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

    Depositor

    Frank Albert

    Article

    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

    Insert

    20mer CASS5a guide (gCASS5a) and 5' sgRNA
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBstSK+IRES-V5-IPpoI

    Plasmid

    #206321

    Purpose

    A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

    Depositor

    Brian McStay

    Article

    van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

    Insert

    IRES element-V5 epitope tag- IPpoI open reading frame
    

    Use

    Mrna production

    Tags

    V5-IPpoI fusion protein

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AY10_pS.Donor.R5.TS

    Plasmid

    #100292

    Purpose

    CCR5-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    18 bp RFLP (MluI+KspAI)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIK198

    Plasmid

    #65629

    Purpose

    "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFP122

    Plasmid

    #21669

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    Lacz (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been modified, the E…

    Promoter

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

21. Bxb1-GA-UbC-mStayGold

    Plasmid

    #241378

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with UbC promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available Since

    Sept. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Bxb1-GA-CBh-mStayGold

    Plasmid

    #241379

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CBh promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Sept. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Bxb1-GA-Bactin-mStayGold

    Plasmid

    #241380

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with B-actin promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Bactin

    Available Since

    Sept. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Bxb1-GA-RSV-mStayGold

    Plasmid

    #241381

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with RSV promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available Since

    Sept. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Bxb1-GA-CMV-mStayGold

    Plasmid

    #241383

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CMV promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Bxb1-GA-CpG-free-mStayGold

    Plasmid

    #241386

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CpG-free promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CpG-free

    Available Since

    Sept. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Bxb1-GT-hEF1a-mStayGold

    Plasmid

    #241388

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with hEF1a promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a

    Available Since

    Sept. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Bxb1-GT-UbC-mStayGold

    Plasmid

    #241389

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with UbC promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available Since

    Sept. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Bxb1-GT-CBh-mStayGold

    Plasmid

    #241390

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CBh promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Sept. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Bxb1-GT-Bactin-mStayGold

    Plasmid

    #241391

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with B-actin promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Bactin

    Available Since

    Sept. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Bxb1-GT-PGK-mStayGold

    Plasmid

    #241392

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with PGK promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Bxb1-GA-hEF1a-mStayGold

    Plasmid

    #241377

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with hEF1a promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Bxb1-GA-ShortCAG-mStayGold

    Plasmid

    #241385

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with ShortCAG promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ShortCAG

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Bxb1-GA-PGK-mStayGold

    Plasmid

    #241384

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with PGK promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Bxb1-GA-SV40-mStayGold

    Plasmid

    #241382

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with SV40 promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Bxb1-GT-CAG-mStayGold

    Plasmid

    #241387

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CAG promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV_CK8e_Cas9_miniPA

    Plasmid

    #226141

    Purpose

    AAV construct for HITI insert with CK8e promoter and SpCas9

    Depositor

    Christopher Nelson

    Article

    Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

    Insert

    Cas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CK8e

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pX459_LIG4_Exon3

    Plasmid

    #225363

    Purpose

    pX459 plasmid encoding the sgRNA protospacer sequence “5’-GCATAATGTCACTACAGATC-3’ to target the LIG4 gene.

    Depositor

    Sujatha Jagannathan

    Article

    Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

    Insert

    LIG4 (LIG4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. POC1550

    Plasmid

    #226497

    Purpose

    pMOBC360_VM_C1 assembled plasmid (Chloramphenicol R) containing four inserts (POC1546-POC1549) assembled in POC1525 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. POC1546

    Plasmid

    #226487

    Purpose

    pMOBK360_VL_N1 assembled plasmid (Kanamycin R) containing four inserts (POC1535-POC1538) assembled in POC1518 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    ssembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. POC1547

    Plasmid

    #226489

    Purpose

    pMOBK360_VM_N2 assembled plasmid (Kanamycin R) containing three inserts (POC1539-POC1541) assembled in POC1519 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. POC1548

    Plasmid

    #226490

    Purpose

    pMOBK360_VM_N3 assembled plasmid (Kanamycin R) containing two inserts (POC1542-POC1543) assembled in POC1519 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. POC1549

    Plasmid

    #226491

    Purpose

    pMOBK360_VR_N4 assembled plasmid (Kanamycin R) containing two inserts (POC1544-POC1545) assembled in POC1520 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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44. Bxb1-GA_CAG-rtTA-T2A-mTagBFP2

    Plasmid

    #229797

    Purpose

    Bxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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45. Bxb1-GA_AIO-TetOn_mScarlet_Convergent

    Plasmid

    #229790

    Purpose

    Bxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

    Plasmid

    #229800

    Purpose

    Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mGreenLantern; mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLP-sfgfp

    Plasmid

    #209994

    Purpose

    Contains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLPT1

    Plasmid

    #209995

    Purpose

    Contains Level 0 Part: Terminator (TB1006) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    Terminator (TB1006)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLPS1

    Plasmid

    #209992

    Purpose

    Contains Level 0 Part: Ribosome binding site (RB0034) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    RBS (RB0034)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pL0_DE_mTurquoise2

    Plasmid

    #202155

    Purpose

    Level 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    mTurquoise2 gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    Aug. 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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