We narrowed to 1,229 results for: Aspa

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pcDNA3/hArf6(T27N)-HA

Plasmid

#79426

Purpose

Expresses C-terminally HA-tagged Arf6(T27N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 27 to Asparagine

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMega-MaFRSA

Plasmid

#200226

Purpose

The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..

Depositor

Alanna Schepartz

Article

Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

Inserts

Pyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA

Tags

none

Expression

Bacterial

Mutation

mutated Asparagine 166 to Alanine and Valine 168 …

Promoter

proK-lacO and tacI

Available Since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Mfn2-HA S27D

Plasmid

#139194

Purpose

Expresses C-terminal HA tagged human Mfn2 S27D in mammalian cells

Depositor

Allan Weissman

Article

Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

Insert

MFN2 (MFN2 Human)

Tags

2HA

Expression

Mammalian

Mutation

Serine 27 to Aspartic Acid

Promoter

CMV promoter

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrasnatG12D

Plasmid

#206842

Purpose

To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Aspartic Acid

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-C2m2-SNAP

Plasmid

#208791

Purpose

For AAV production to express C2m2-SNAP in astrocytes

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2m2-SNAP (Mfge8 Mouse)

Use

AAV

Tags

SNAP-tag

Expression

Mammalian

Mutation

Mutated 24 and 45 lysine to asparagine in C2 doma…

Promoter

GFAP promoter

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_DW164AA USP7

Plasmid

#131252

Purpose

Mammalian expression of N-terminally Myc-tagged DW164AA USP7

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Contains a point mutation that converts USP7 aspa…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-ISG15 C78SN13Y

Plasmid

#165106

Purpose

bacterial expressio of a GST fusion of Human ISG15 C78SN13Y

Depositor

Arthur Haas

Article

Narasimhan et al J Biol Chem. 2005 Jul 22. 280(29):27356-

Insert

ISG15 (ISG15 Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

C78SN13Y; The sequence of ISG15-C78S/N13Y contai…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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LMPd Ametrine Got2 shRNA#3

Plasmid

#220593

Purpose

Retroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Ma et al bioRxiv [Preprint]. 2023 Jun 11:2023.06.

Insert

Got2 shRNA (Got2 Mouse)

Use

Retroviral

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-jYCaMP1

Plasmid

#135422

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection

Depositor

Kaspar Podgorski

Article

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Insert

jYCaMP1

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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RANLS-IETD-BNES

Plasmid

#60972

Purpose

Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddRFP A and ddRFP B

Tags

NES sequence LALKLAGLDIGS and triplicated NLS seq…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
pINDUCER20 MEF2C S222D

Plasmid

#107234

Purpose

MEF2C phosphomimetic mutant serine to aspartic acid

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C

Use

Lentiviral

Expression

Mammalian

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-D2512H

Plasmid

#69017

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-D2512H (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Aspartate 2512 to Histidine

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pGL4.23 A82V/T230A/D637G 2014 EBOV + Mucin-Like Domain

Plasmid

#86020

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Changed alanine 82 to valine, threonine 230 to al…

Promoter

CMV IE

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23 D637G 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86024

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain: Changed aspartic acid 63…

Promoter

CMV IE

Available Since

April 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Flag-SOD1-D91A

Plasmid

#235664

Purpose

Express human SOD1-D90A in mammalian cells

Depositor

Chen Seng Ng

Article

Hiew et al Commun Biol. 2025 Mar 2;8(1):347. doi: 1

Insert

SOD1-D91A (SOD1 Human)

Tags

Flag-tag at N-terminal

Expression

Mammalian

Mutation

changed Aspartic acid 91 to alanine

Promoter

CMV promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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LMPd Ametrine Got1 shRNA#1

Plasmid

#220592

Purpose

Retroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Ma et al bioRxiv [Preprint]. 2023 Jun 11:2023.06.

Insert

Got1 shRNA (Got1 Mouse)

Use

Retroviral

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-RepoMan-S893D

Plasmid

#204995

Purpose

Expresses shRNA resistant GFP-RepoMan S893D Mutant

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell division cycle associated 2 (CDCA2 Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Serine 893 to Aspartic acid

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-727pUC19-tNGFR-P2A-WASP

Plasmid

#186089

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

WASP (WAS Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CSNK1A1 G40N-P2A-Blast

Plasmid

#123321

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Blast casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

changed Glycine 40 to Asparagine

Promoter

CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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N295A (EcN), E166Q (EcN) MetNI LmH

Plasmid

#118269

Purpose

N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure

Depositor

Douglas Rees

Article

Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

Inserts

MetI (metI Escherichia coli)
MetN (E166Q ; N295A) (metN Escherichia coli)

Tags

6x His Tag

Expression

Bacterial

Mutation

Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…

Promoter

T7 Promoter

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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