We narrowed to 24,925 results for: crispr
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Plasmid#79615PurposeExpresses dCas9-PmCDA1 in yeast cellsDepositorPublicationInsertSpCas9
TagsPmCDA1ExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1Available sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC19-dual-scafffold-2.0 (pBP49)
Plasmid#218931PurposeThe dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdual scaffolds for CRISPR
UseCRISPRAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct
Plasmid#176836PurposeTargeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
UseUnspecifiedMutationSee Depositor Comments BelowPromoterTet operator (tetracycline inducible expression)Available sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Cas9-pA-Cry-GFP
Plasmid#168239Purpose"neutrophil specific cas9 expression in zebrafish with green lens"DepositorInsertCas9
UseZebrafish expressionAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSP2292
Plasmid#70707PurposeBacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationE782K, N968K and R1015H in SaCas9PromoterT7Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GluN2A (px330:2-guideCas9)
Plasmid#169428PurposeExpression of Cas9 and 2 guidesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspCas9
UseCRISPRExpressionMammalianAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CFP-SaCas9-HF (without sgRNA)
Plasmid#134470PurposeHuman expression vector for SaCas9-HF variant.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStaphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
UseCRISPRTagsSV40NLS-3xFLAGExpressionMammalianMutationR245A, N413A, N419A, R654APromoterCAGAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSL0426 (CRISPR with spc3)
Plasmid#130642PurposeExpresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.DepositorInsertCRISPR(spacer-3)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-hCASeGFP-pA
Plasmid#59766PurposeExpresses CAS9-eGFP fusion protein in mammalian cells.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCAS9
TagsFlag and eGFPExpressionMammalianPromoterCAGAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVNP2.0-SpCas9
Plasmid#206880PurposeExpresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domainDepositorAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9
Plasmid#80425PurposeUsed for in vitro transcription of SpCas9 mRNA.DepositorInsertSpCas9
UseCRISPRTagsnoneMutationcodon optimized for humanPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PX544 SpCas9(del1098-1368)
Plasmid#58889PurposeTruncated form of SpCas9 without C' domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianMutationdel amino acids 1098-1368Available sinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
PX543 SpCas9(del175-307)
Plasmid#58888PurposeTruncated form of SpCas9 without REC2 domainDepositorInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianMutationdel amino acids 175-307Available sinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
SpCas9
Plasmid#188489PurposeExpresses FLAG tagged SpCas9DepositorInsertCas9
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAS9-TPC
Plasmid#61478PurposeBinary expression vector with A.th. codon-optimized Cas9 for conventional cloningDepositorInsertCas9
UseCRISPRExpressionPlantPromoterPcUbi4-2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHis-parallel1-NLSH2BCas9
Plasmid#112065PurposeExpression and purification of NLSH2BCas9 for fungal gene editingDepositorInsertNLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
UseCRISPRTagsHis tagExpressionBacterialMutationCas9 was codon optimized for Aspergillus nigerPromoterpromoter in vectorAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEJS1089: mini-AAV.sgRNA.Nme2Cas9
Plasmid#159536PurposeDelivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 KbDepositorInsertNme2Cas9 with single guide RNA cassette
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianPromoterU1aAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-HygR-EGFP
Plasmid#167188PurposeLentiviral expression of sgRNA with hygromycin resistance gene and EGFPDepositorInsertHygromycin B phosphotransferase and EGFP
UseCRISPR and LentiviralExpressionMammalianAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGES401
Plasmid#190198PurposeFor CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selectionDepositorInsertspCas9
UseCRISPRTags3xFlagExpressionPlantMutationplant-codon optimizedPromoterGlycine max elongation factor 1A(pM4)Available sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_Blasto
Plasmid#167856PurposePiggyBac compatible plasmid expressing spCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
UseCRISPRAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only