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pNL-CEF-CD79B-YFP

Plasmid

#187006

Purpose

Expression of human CD79B as YFP fusion protein

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

CD79B (CD79B Human)

Use

Lentiviral

Tags

YFP

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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MIGR1-Cdc42-1

Plasmid

#179517

Purpose

Retroviral expression of Cdc42 splice variant 1

Depositor

Bo Porse

Article

Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

Insert

Cdc42 splice variant 1 cDNA (CDC42 Human)

Use

Retroviral

Tags

IRES-EGFP

Promoter

LTR

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-Halo-CD4-bla

Plasmid

#179445

Purpose

Donor vector to knock in Halotag C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

HaloTag (Halo7 Synthetic)

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

Plasmid

#159283

Purpose

A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Inserts

Human INS sgRNA
Human INS guide RNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-100RPU.2-ETN-BGHpA-cHS4-100RPU.2-ETN-lox2272

Plasmid

#154834

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of etanercept

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

ETN
ETN

Use

Cre/Lox

Expression

Mammalian

Promoter

100RPU.2

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC deltaLZ_P2A_Hygro_Barcode

Plasmid

#120509

Purpose

Barcoded lentiviral vector to express MYC deltaLZ in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC deltaLZ (MYC Human)

Use

Lentiviral

Mutation

Deletion of leucine zipper motif

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC deltaHLH_P2A_Hygro_Barcode

Plasmid

#120508

Purpose

Barcoded lentiviral vector to express MYC deltaHLH in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC deltaHLH (MYC Human)

Use

Lentiviral

Mutation

Deletion of helix-loop-helix motif

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3con-miR143Bul

Plasmid

#21119

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-143 sensor

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pEF Itk K390R myc

Plasmid

#20707

Depositor

Leslie Berg

Article

Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

Insert

Itk K390R (Itk Mouse)

Tags

myc

Expression

Mammalian

Mutation

Kinase Dead. K390R.

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pMB84 (Nf2 C-term)

Plasmid

#12169

Depositor

Tyler Jacks

Article

McLaughlin et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Nf2 C-terminal half (Nf2 Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

C-terminal half of Nf2 cDNA

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only
AAV-hT7

Plasmid

#252215

Purpose

AAV6 capsid variant evolved

Depositor

Justin Eyquem

Article

Nyberg et al Nature. 2026 Mar 18. doi: 10.1038/s41586

Insert

AAV-hT7 rep/cap

Use

AAV

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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pSLQ11453

Plasmid

#183206

Purpose

PB-NFKB-BFP-Triplex-crAAVS1_4-SV40pA

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crAAVS1_4)

Use

CRISPR

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pRSETA_ionRD

Plasmid

#172924

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRD

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ionRE

Plasmid

#172932

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRE

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-3xMyc

Plasmid

#129569

Purpose

Empty backbone for Myc Tag

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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MTK2_006

Plasmid

#123701

Purpose

Encodes pSV40 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSV40

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pIG-721 CTLA4-GFP T124P

Plasmid

#186126

Purpose

CTLA4 gene knockin with mutation

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CTLA4 (CTLA4 Human)

Use

CRISPR

Mutation

T124P

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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CD44Z-c011

Plasmid

#175455

Purpose

Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.

Depositor

Opher Gileadi

Insert

CD44 (CD44 Human)

Tags

His6

Expression

Mammalian

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-NHASt-codoptUS2

Plasmid

#135102

Purpose

Codon-optimized HSV-1 US2 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 US2 codon optimized

Tags

HA-Strep-Strep

Expression

Mammalian

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pOEM1_pCMV:bmp4-pPH:vsvged

Plasmid

#111157

Purpose

Baculovirus rescue vector, VSVGED pseudotype, hShh

Depositor

Elly Tanaka

Article

Wagner et al Dev Cell. 2017 Mar 27;40(6):608-617.e6.

Insert

hBmp4 (BMP4 Human)

Use

Baculoviral rescue shuttle vector

Expression

Insect and Mammalian

Mutation

wt

Promoter

CMV

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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