We narrowed to 12,204 results for: 112

1. pET-28a RUVBL2 Delete

   Plasmid

   #159143

   Purpose

   Expression and protien purification of a mutated RUVBL2

   Depositor

   Eric Erquan Zhang

   Article

   Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

   Insert

   RUVBL2 (RUVBL2 Human)
   

   Expression

   Bacterial

   Mutation

   amino acid 2-456, Thr127-Glu233 mutated to GPPG

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEx1-pEF-H2B-mCherry-T2A-rTetR-EED

   Plasmid

   #78101

   Purpose

   Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

   Depositor

   Michael Elowitz

   Article

   Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

   Insert

   H2B-mCherry-T2A-rTetR-EED (EED Human, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   rTetR

   Expression

   Mammalian

   Promoter

   pEF alpha

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. CCR4-DuET

   Plasmid

   #213200

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CCR4 (CCR4 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221_SLC39A9

   Plasmid

   #132189

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC39A9 (SLC39A9 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 28, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pNK7187

   Plasmid

   #219761

   Purpose

   MoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   PpASCL
   

   Expression

   Plant

   Promoter

   p35s_0.4kb - 5'UTR TMV omega

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CX3C1-DuET

   Plasmid

   #213217

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CX3C1 (CX3CR1 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE_HRASV12_Hyg

   Plasmid

   #195143

   Purpose

   HRas G12V overexpression vector with hygroymycin resistance

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   hRAS (HRAS Human)
   

   Expression

   Mammalian

   Mutation

   G12V

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hsyn-flex-Voltron-ST

   Plasmid

   #119036

   Purpose

   Cre-dependant expression of soma-localized Voltron in neurons

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

   Has Service

   AAV1

   Insert

   Voltron-ST
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   hsyn

   Available Since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. ADORA2A-DuET

   Plasmid

   #213174

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADORA2A (ADORA2A Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GPRC5B-DuET

    Plasmid

    #213296

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPRC5B (GPRC5B Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAF92

    Plasmid

    #135929

    Purpose

    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Insert

    mCherry fused to TNFSF9
    

    Use

    Transposon

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-SKP1-T131D

    Plasmid

    #213600

    Purpose

    Expresses human SKP1 T131D mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

    Insert

    human Flag tagged SKP1-T131D (SKP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDB-His-MBP-mGSDMD

    Plasmid

    #123365

    Purpose

    Expression of full-length mouse GSDMD in E. coli

    Depositor

    Hao Wu

    Article

    Orning et al Science. 2018 Nov 30;362(6418):1064-1069

    Insert

    GSDMD (Gsdmd Mouse)
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. CYSLTR1-DuET

    Plasmid

    #213225

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CYSLTR1 (CYSLTR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lentiCRISPR - EGFP sgRNA 4

    Plasmid

    #51763

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 4
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag-SKP1-T131E

    Plasmid

    #213601

    Purpose

    Expresses human SKP1 T131E mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

    Insert

    human Flag tagged SKP1-T131E (SKP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiCRISPR - EGFP sgRNA 5

    Plasmid

    #51764

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 5
    

    Use

    CRISPR and Lentiviral

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. plentiCRISPv2_FDX1-3

    Plasmid

    #184490

    Purpose

    Lentivirus all in one CRISPR vector targeting human FDX1 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    FDX1 (FDX1 Human)
    

    Use

    Lentiviral

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. plentiCRISPv2_FDX1-1

    Plasmid

    #184488

    Purpose

    Lentivirus all in one CRISPR vector targeting human FDX1 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    FDX1 (FDX1 Human)
    

    Use

    Lentiviral

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. OPRK1-DuET

    Plasmid

    #213359

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPRK1 (OPRK1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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