We narrowed to 13,795 results for: FLU
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Plasmid#62848PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). attP upstream of attB in this plasmid.DepositorInsertReporter_13_switchBP
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUCSh3-mRFP
Plasmid#170983PurposeEncodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.DepositorInsertmRFP
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVH232-Tier1-PmPGK1-mRuby2
Plasmid#169523PurposeTier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).DepositorInsertPPGK-driven monomeric red fluorescent protein mRuby2
ExpressionMammalianPromoterPPGKAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-GFPγ-SpHis5
Plasmid#60781PurposeYeast C-terminal tagging vector, which creates gene fusions with GFPγ using SpHis5 selectionDepositorInsertGFPγ
UseYeast integratingMutationT65C S72A V163A I167T L231HAvailable SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRL WS1.6 GFP
Plasmid#36249DepositorAvailable SinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
iRFP713-FRB-PIP5K1A
Plasmid#202743Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorInsertiRFP713:MTOR(2021-2113):GGSA2:PIP5K1A (PIP5K1A Human)
ExpressionMammalianAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
iRFP713-FRB-Mss4-Kina
Plasmid#202744Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorInsertiRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)
ExpressionMammalianAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYFP (GB0053)
Plasmid#68197PurposeProvides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid partDepositorInsertYFP coding region
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3
Plasmid#65451PurposepILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.DepositorTypeEmpty backboneTagsyEGFP reporterExpressionYeastAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pITGFP3
Plasmid#65452PurposepITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.DepositorTypeEmpty backboneTagsyEGFP-CLN2PEST reporterExpressionYeastAvailable SinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_AKAR5Δ
Plasmid#119912PurposeMammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5Δ
Tags6xHisExpressionMammalianAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
sspa-GCaMP6s
Plasmid#67559Purposeshort photoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6s
ExpressionMammalianMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…Available SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-mos::NG
Plasmid#138958PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutation8 bp that are in the region that encodes the cata…PromoterUASAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (2)
Plasmid#185852PurposeTesting the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP223-AAV-EFS-GFP-pA
Plasmid#62913PurposeAAV vector backbone designed to express GFP from a EFS promoter. It also contains a poly-Adenylation Signal (pA)DepositorInsertGreen Fluorescent protein (GFP)
UseAAVPromotershort form of Elongation Factor1α (EFS)Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARγ
Plasmid#119915PurposeMammalian expression of the tAKARγ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARγ
ExpressionMammalianAvailable SinceApril 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET-triple mutation-pa-GCaMP6f
Plasmid#67570Purposebacterial expression of a photoactivatable fluorescent calcium reporterDepositorInsertpa-GCaMP6f
TagsHIS-tagExpressionBacterialMutationV115H, L64F, T65SPromoterT7Available SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Crimson/P2A-NLS/HA-HumBeta (pSLIK2)
Plasmid#113857PurposeFor Doxycycline-inducible expression of Humanized Beta synaptase gene, with red fluorescent protein gene E2-Crimson-P2A-Humanized Beta synaptase geneDepositorInsertHumanized Beta
UseLentiviralTagsP2A/CrimsonMutation"humanized" Beta geneAvailable SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDownstream1Lox
Plasmid#164574PurposeIntegration of 1 LoxN site in the genome of P. bergheiDepositorTypeEmpty backboneUseCre/LoxPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP9E3
Plasmid#185842PurposeTesting the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
ExpressionYeastMutationNoneAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only