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pcDNA3-neo-Strep_Flag_Cterm

Plasmid

#102645

Purpose

Epitope tag c-terminus with SBP-Flag moiety

Depositor

Michael E. Wright

Article

Hsiao et al BMC Cancer. 2015 Mar 31;15:204. doi: 10.

Type

Empty backbone

Tags

SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

Expression

Mammalian

Available Since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd3-1D4

Plasmid

#42265

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 3 (Fzd3 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pJOG536

Plasmid

#105423

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Nuclear targeting factor (RanGAP1(WPP)-GFP-BLRP)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pQE30-roGFP1-iL

Plasmid

#83313

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

Insert

roGFP1-iL

Tags

6xHIS

Expression

Bacterial

Mutation

ro1(C48S/S147C/Q204C) plus X insertion, iL betwe…

Promoter

T5

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pTYF-sGFAP-IRES-tdTomato

Plasmid

#166920

Purpose

Empty plasmid backbone; includes IRES-tdTomato and sGFAP promoter

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Type

Empty backbone

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_NES_C1

Plasmid

#85062

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L5L2

Plasmid

#113738

Purpose

Gateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Plasmid

#163062

Purpose

Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

QuasAr2

Use

Gateway entry vector

Tags

mOrange2

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pOGG010

Plasmid

#113984

Purpose

Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

RK2 (IncP)

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBFP (GB0025)

Plasmid

#68193

Purpose

Provides the CDS of the Blue fluorescente protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

BFP coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAX

Plasmid

#202719

Purpose

Mammalian co-expression of membrane-bound Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Inserts

Myr-Pink Flamindo
EGFP-bPAC-CAAX

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP FAT

Plasmid

#50517

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

Insert

protein tyrosine kinase, domain

Tags

GFP

Expression

Mammalian

Mutation

truncated (NT2630-3268)

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
Rix-PGK-Tom-W

Plasmid

#25813

Depositor

Jozsef Kiss, Patrick Salmon

Article

Dayer et al Brain. 2007 Nov . 130(Pt 11):2962-76.

Insert

Rix-PGK-Tom-W

Use

Lentiviral

Mutation

tdTomato from Tsien lab

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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FLAG-mTIP49a

Plasmid

#15357

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

TATA binding protein interacting protein 49 kDa (Ruvbl1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 beta-catenin Y654E

Plasmid

#16073

Depositor

Erin Schuman

Article

Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

Insert

beta-catenin (CTNNB1 Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

Y654E, *(see below)

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP

Plasmid

#67299

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Tiam1-pMagFast2(3x)-iRFP (Tiam1 Mouse, Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pSicoR human DGCR8-2

Plasmid

#14770

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (DGCR8 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pENTR-PpU6P-sgRNA-R4R3

Plasmid

#113739

Purpose

Gateway entry vector containing attR4 and attR3 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L5L4

Plasmid

#113741

Purpose

Gateway entry vector containing attL5 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L1R5

Plasmid

#113737

Purpose

Gateway entry vector containing attL1 and attR5 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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