19,835 results

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pBOB-Lck-GFP

Plasmid

#118738

Purpose

To express membrane targeted GFP

Depositor

John Cooper

Insert

Lck domain (LCK Human)

Use

Lentiviral

Tags

GFP

Mutation

H8N (see comments)

Promoter

CMV

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

Plasmid

#111818

Purpose

Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

Mef2c (Mef2c Mouse)
Tdtomato
Gata4 (Gata4 Mouse)
Tbx5

Use

Cloning intermediate

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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HA-Daxx/pRK5

Plasmid

#52023

Purpose

Expresses HA-Daxx in mammalian cells

Depositor

Xiaolu Yang

Article

Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

Insert

HA-Daxx (DAXX Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP16

Plasmid

#141269

Purpose

Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGWB555

Plasmid

#74885

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLPT119

Plasmid

#85526

Purpose

Repressilator without degradation tags, with integrated reporter

Depositor

Johan Paulsson

Article

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Inserts

mCfp
mVenus
tetR
lacI
cI

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 8, 2017

Availability

Academic Institutions and Nonprofits only

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pVenus-C1-Cdc20 (1534)

Plasmid

#26058

Depositor

Jonathon Pines

Article

Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

Insert

Cdc20 (Cdc20 Human)

Tags

Venus

Expression

Mammalian

Available Since

Aug. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RIPK3

Plasmid

#78815

Purpose

To overexpress RIPK3 in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBLADE(FP6**)-mCherry

Plasmid

#168049

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.

Depositor

Barbara Di Ventura

Article

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Inserts

Blue light-inducible AraC dimers in Escherichia coli
mCherry

Expression

Bacterial

Promoter

J23101** and PBAD

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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ePPE

Plasmid

#183095

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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plx304-PHB-V5

Plasmid

#122230

Purpose

Expresses human PHB attached to a V5 tag

Depositor

Alex Kentsis

Article

Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

Insert

Prohibitin (PHB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Nst-CAG-DEST

Plasmid

#80489

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pET-3c-rhVTN_FL

Plasmid

#30223

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin without deletions (VTN Human)

Expression

Bacterial

Mutation

No deletions

Available Since

May 24, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223-ACVR2B

Plasmid

#23401

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ACVR2B (ACVR2B Human)

Use

Gateway donor vector

Mutation

A333G, 1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Lys-eMagB-iRFP

Plasmid

#162249

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

p18-eMagB-iRFP

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAPK1_WT

Plasmid

#82145

Purpose

Gateway Donor vector containing MAPK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK1 (MAPK1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJR100

Plasmid

#187240

Purpose

Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for Perturb-seq with mU6 promoter

Use

Lentiviral

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pEPkan-S

Plasmid

#41017

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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BRCA2 A11.2 gRNA

Plasmid

#90550

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA2 (Guide Designation A11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro

Plasmid

#31845

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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