We narrowed to 59,496 results for: %s

1. LbCas12a-2C-NLS in pCSDest

   Plasmid

   #126638

   Purpose

   Expresses LbCas12a-2C-NLS in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   LbCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Promoter

   CMV IE94 promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR8/GW/TOPO-TBX20a

   Plasmid

   #98620

   Purpose

   Gateway entry TOPO TA cloning vector for TBX20a

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   Tbx20a (Tbx20 Mouse)
   

   Use

   Topo ta cloning vector

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQHA-USP7 CS puroR

   Plasmid

   #46754

   Purpose

   Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C223S--catalytically inactive

   Promoter

   CMV

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGST2-GST-TEV-NDP52

   Plasmid

   #187828

   Purpose

   Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NDP52 (CALCOCO2 Human)
   

   Tags

   GST-TEV

   Expression

   Bacterial

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC129: pAAV.CMV-CasRx mCh

   Plasmid

   #203442

   Purpose

   Plasmid expressing active RfxCas13d with mCherry reporter for investigating CasRx activity

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Insert

   RfxCas13d-T2A-mCherry
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT-TO-EGFR*-CUbo

   Plasmid

   #212821

   Purpose

   Constitutive or doxycycline-inducible expression of EGFR*-CUbo in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   EGFR* (EGFR Budding Yeast, Human)
   

   Tags

   CUbo

   Expression

   Mammalian

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIneo-AR-S256A

   Plasmid

   #171227

   Purpose

   Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser256Ala (AR-S256A)

   Depositor

   Steven Balk

   Article

   Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

   Insert

   human androgen receptor Ser256Ala mutant (AR Human)
   

   Expression

   Bacterial and Mammalian

   Mutation

   AR-Ser256Ala (AR-S256A)

   Promoter

   CMV

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIneo-AR-S308A

   Plasmid

   #171229

   Purpose

   Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser308Ala (AR-S308A)

   Depositor

   Steven Balk

   Article

   Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

   Insert

   human androgen receptor Ser308Ala mutant (AR Human)
   

   Expression

   Bacterial and Mammalian

   Mutation

   AR-Ser308Ala (AR-S308A)

   Promoter

   CMV

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIneo-AR-S90A

   Plasmid

   #171225

   Purpose

   Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser90Ala (AR-S90A)

   Depositor

   Steven Balk

   Article

   Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

   Insert

   human androgen receptor Ser90Ala mutant (AR Human)
   

   Expression

   Bacterial and Mammalian

   Mutation

   AR-Ser90Ala (AR-S90A)

   Promoter

   CMV

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMy-MouseCTCFbiotag-T2A-mOrange

    Plasmid

    #50564

    Purpose

    Retroviral vector endoding for mOrange and mouse CTCF with a short biotinylation substrate (biotag, Kimetal.,2009) fused to its C-term

    Depositor

    Rafael Casellas

    Article

    Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

    Inserts

    Ampicilin
    Mouse CTCF Exons
    

    Use

    Retroviral

    Available Since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcGFP1-N1-RvCAHS3-GS

    Plasmid

    #192460

    Purpose

    Expression of RvCAHS3-GS_linker-AcGFP1 in mammalian cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS3
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV.EF1α.mEmerald.CD9.miR9T

    Plasmid

    #170452

    Purpose

    Lentiviral plasmid that encodes mEmerald-CD9 fusion protein with microRNA 9 tandem cassette to restrict expression to microglia with EF1-alpha promoter.

    Depositor

    Tsuneya Ikezu

    Article

    Clayton et al Mol Neurodegener. 2021 Mar 22;16(1):18.

    Insert

    CD9 (CD9 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    EF1a

    Available Since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 c-SRC (1-249)

    Plasmid

    #42204

    Depositor

    Robert Lefkowitz

    Article

    Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

    Insert

    c-SRC (1-249) (SRC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    contains amino acids 1-249

    Promoter

    CMV

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pET-Duet1-His-TEV-mCherry-OPTN WT

    Plasmid

    #190191

    Purpose

    Plasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    optineurin (OPTN Human)
    

    Tags

    6xHis, TEV, and mCherry

    Expression

    Bacterial

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSKC12 - DelQ

    Plasmid

    #110493

    Purpose

    Monitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.

    Depositor

    Shankar Balasubramanian

    Article

    Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

    Insert

    NRAS 5'- DelQ (bp 30-254) (NRAS Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Mutation

    Deletion of the first 29 base pairs of the NRAS 5…

    Promoter

    T7

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFB-EPCP5

    Plasmid

    #228879

    Purpose

    Expression of Z-tagged LRRK2 KW using Baculoviruses

    Depositor

    Sebastian Mathea

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Tags

    His-Z-TEV

    Expression

    Insect

    Mutation

    Amino acids deleted 1-1846

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-SYN-L-sDarken

    Plasmid

    #184798

    Purpose

    Low affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    L-sDarken
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ265E9

    Plasmid

    #213472

    Purpose

    Gateway entry clone (attL1 & attR5) TadDE-32aa linker-zSpCas9-D10A-2xUGI for C-T and A to G base editing

    Depositor

    Yiping Qi

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Insert

    TadDE-32aa linker-zSpCas9-D10A-2xUGI
    

    Use

    CRISPR; Gateway compatible tadde-32aa linker-zspc…

    Tags

    NO

    Expression

    Plant

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. ACE2-Fc

    Plasmid

    #164222

    Purpose

    Expresses soluble , dimeric Fc-His fusion protein for the human ACE2 extracellular domain, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    ACE2 extracellular domain (ACE2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. POLR2A-N CRISPR pX330

    Plasmid

    #124495

    Purpose

    A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A

    Depositor

    Masato Kanemaki

    Article

    Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

    Insert

    POLR2A targeting CRISPR
    

    Use

    CRISPR

    Promoter

    U6 promoter

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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