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HA-cNM2B-Flag

Plasmid

#236534

Purpose

For expression of NM2B (dog) - with HA and FLAG tags in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

MYH10

Tags

FLAG and HA

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SARS-COV-2-5UTR-GLuc

Plasmid

#199592

Purpose

Express SARS-CoV-2 5 UTR in mammalian cells.

Depositor

Jingxin Wang

Article

Tang et al Nat Commun. 2025 Jan 8;16(1):483. doi: 1

Insert

SARS-CoV-2 5UTR-GLuc

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FRB-Fis1 (no mt-mKeima)

Plasmid

#223795

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FRB-Fis1

Use

Lentiviral

Tags

FRB

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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tdiRFP-BFP reporter

Plasmid

#190184

Purpose

The cre-activatable fluorescence reporter

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

tdiRFP
TagBFP2

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-PLAP

Plasmid

#222308

Purpose

Synchronize the trafficking of PLAP from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and PLAP fused to SBP-EGFP (ALPP Human)

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

Plasmid

#228400

Purpose

Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

NES/PKI-mNeonGreen2x-NLS/FUS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro

Plasmid

#228398

Purpose

Encodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

NLS/cMyc-AcGFP2x-NES/iKB2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-E-cadherin(Canis)-exon1 6-28 gRNA

Plasmid

#209922

Purpose

A knock-out vector for the dog CDH1

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

A gRNA targeting the dog CDH1 gene.

Use

CRISPR and Lentiviral

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-p120catenin(Canis)-exon4 116-138 gRNA

Plasmid

#209923

Purpose

A knock-out vector for the dog CTNND1

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

A gRNA targeting the dog CTNND1 gene.

Use

CRISPR and Lentiviral

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-TGFa-mNeonGreen

Plasmid

#209913

Purpose

To monitor the expression of TGFα, the plasmid encodes a recombinant TGFα fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-mNeonGreen (TGFA Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd

Plasmid

#216171

Purpose

Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_Seq17_VS33_4.26_ChimeraIntron_3NLS_RFP_WPRE_polyA

Plasmid

#220225

Purpose

AAV-STARR-seq validation vector containing VS33 enhancer

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Insert

Enhancer (VS33)

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-WT1 +KTS C terminal HA

Plasmid

#217640

Purpose

Entry vector with WT1 +KTS mutant with C terminal HA for gateway cloning

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 +KTS isoform (WT1 Human)

Use

Gateway cloning

Tags

HA

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-WT1 -KTS C terminal HA

Plasmid

#217642

Purpose

Entry vector with WT1 -KTS mutant with C terminal HA for gateway cloning

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 -KTS isoform (WT1 Human)

Use

Gateway cloning

Tags

HA

Mutation

p.408_410delKTS

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4562

Plasmid

#200780

Purpose

Pflp-18 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GtAR2

Tags

mCherry

Expression

Worm

Promoter

Pflp-18

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4609

Plasmid

#200781

Purpose

Ppdf-1 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVB and other neurons expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GtACR2

Tags

mCherry

Expression

Worm

Promoter

Ppdf-1

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4677

Plasmid

#200782

Purpose

Pnpr-4 ins-22::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 GFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ins-22 (ins-22 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pnpr-4

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2829

Plasmid

#199693

Purpose

Pcex-1 tomm-20::miniSOG UrSL mCherry unc-54 3' UTR C.elegans RIM neuron expression of miniSOG RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

miniSOG

Tags

RFP

Expression

Worm

Promoter

Pcex-1

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)

Plasmid

#208869

Purpose

For mammalian expression of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

HA

Expression

Mammalian

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)

Plasmid

#208872

Purpose

For expression in E. coli of NAP1 (delta-NDP52)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

MBP-TEV

Expression

Bacterial

Mutation

S37K/A44E (disrupts NDP52 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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