We narrowed to 159,093 results for: Des
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Plasmid#229817PurposeA gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.DepositorTypeEmpty backboneUseTol2 destination vectorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only
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Desmoplakin II acceptor only control (F40-based tension sensor)
Plasmid#118719PurposeThe acceptor (mCherry) only control for the F40-based human desmoplakin II tension sensor can be co-expressed with the donor (YPet(short)) only control to determine intermolecular FRET.DepositorInserthuman Desmoplakin II-[YPet(short)(Y67G)-F40-mCherry] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)(Y67G)-F40-mCherryExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterCMVAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II acceptor only control (F40-based no force control)
Plasmid#118720PurposeThe acceptor (mCherry) only control for the no force control of the F40-based human desmoplakin II tension sensor can be co-expressed with the donor only control to determine intermolecular FRET.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)(Y67G)-F40-mCherry] (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherry(Y72L)ExpressionMammalianMutationtruncation after aa1353; Y67G mutation in YPet(sh…PromoterCMVAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry
Plasmid#69233PurposeR1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)DepositorInsertSpCas9
UseCRISPRTags2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…ExpressionMammalianMutationArginine 1335 is changed to LysineAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3
Plasmid#69231PurposeExpresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cellDepositorInsertTS3 TALE
UseCRISPRTags2X NLS and NLS-3XHA-NLSExpressionMammalianAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides
Plasmid#172506PurposeEncodes DisCas7-11 protein alone with a golden gate site for spacer cloningDepositorInsertDisCas7-11 bacterial expression vector with golden gate site for guides
ExpressionBacterialAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)
Plasmid#229839PurposeA CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.DepositorInsertLuciferase
ExpressionMammalianAvailable SinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)
Plasmid#192124PurposepHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)DepositorInsertpEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
UseLentiviralAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Rac-WT(guide resistant)-2a-mcherry-U6ac-Rac guides
Plasmid#168242Purpose"Rescue Rac expression in neutrophil specific knockout"DepositorInsertRac(guide resistant)-WT
UseZebrafish expressionTagsmcherryPromoterLyzCAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB-TetOn-DEST-EFS-mODC-rtTA-IRES-NEO (JDW 931)
Plasmid#242578PurposePiggyBac transposon flanked, Tet-on, gateway destination vector.DepositorInsertattR1-CmR-ccdB-attR2
ExpressionMammalianAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII
Plasmid#192705PurposePlasmid for the Expression of the E3 complex with an FII (F32A, I35A, and I36A) mutation. SMC Internal reference: SMC1728DepositorTags-GFP-TEV-StrepII and 10x-His-TEVExpressionInsectMutationF32A-I35A-I36AAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides
Plasmid#168243Purpose"Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout"DepositorInsertRac(guide resistant)-DN
UseZebrafish expressionTagsmcherryPromoterLyzCAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Rac-CA(guide resistant)-2a-mcherry-U6ac-Rac guides
Plasmid#168244Purpose"Rescue Rac-CA expression in neutrophil specific knockout"DepositorInsertRac(guide resistant)-CA
UseZebrafish expressionTagsmcherryPromoterLyzCAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP14461: pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BarcodeS-BGHpA (Alias: CN4461)
Plasmid#236600PurposeBarcoded enhancer AAV vector for multiplexed screening applications; AiE0779m_3xC2 is an enhancer active in striatal D1-MSNsDepositorInsertSYFP2
UseAAVAvailable SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene B protein LCD (1-136)
Plasmid#235001PurposeBacterial expression of N-terminally 6His tagged Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene B protein LCDDepositorInsertBeckwith-Wiedemann syndrome chromosomal region 1 candidate gene B protein (LOC129490717 Human)
Tags6xHis-TEVExpressionBacterialPromoterT7Available SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGG-GOI-DE-TurboID
Plasmid#209388PurposeGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneExpressionPlantMutationTurboID gene sequence C249AAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-HRP-C
Plasmid#40163DepositorInsertHorseradish peroxidase
UseColi cloning vectorTagsSix histidineAvailable SinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-synaptophysin-Lime
Plasmid#201016PurposeExpresses Lime in synaptic vesicle lumen by fusion to synaptophysinDepositorAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR-spondin 1
Plasmid#128844Purposebacterial expression of pR-spondin 1DepositorAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-IgK-pHluorin-TM-mRuby
Plasmid#185546PurposeExpresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoterDepositorInsertIgK-pHluorin-TM-mRuby
ExpressionMammalianPromoterCMVAvailable SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-IRES-deltaNGFR
Plasmid#72930Purposeretroviral expression with FACS-selectable marker (extracellular part of NGFR)DepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
CamKII-LGI1-pHluorin
Plasmid#185537PurposeExpresses LGI1-pHluorin under the CamKII promoterDepositorAvailable SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
CamKII-LGI1-pHmScarlet
Plasmid#185538PurposeExpresses LGI1-pHmScarlet under the CamKII promoterDepositorAvailable SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHRW-CRY2-VHH(GFP)
Plasmid#126022Purposeexpress RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogeneticsDepositorInsertCRY2 PHR-VHH(GFP)
TagsRFPExpressionInsectMutationWTPromoterHsp70Available SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-pHluorin-Synaptotagmin1
Plasmid#224430PurposeExpression of Synaptotagmin1-pHluorin for optical monitoring of synaptic vesicle cycling in neuronsDepositorInsertSynaptotagmin1-pHluorin
UseLentiviralTagspHluorinExpressionMammalianPromoterCaMKIIAvailable SinceJuly 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-trc- YFP
Plasmid#186569Purposep15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
ExpressionBacterialPromotertrc promoterAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15ABAD- YFP
Plasmid#186571Purposep15A ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
ExpressionBacterialPromoterBAD promoterAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-YFP
Plasmid#186563Purposep15A ori, promotorless, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
TagsNoneExpressionBacterial and MammalianMutationNonePromoterPromotorlessAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV CytoBAS
Plasmid#62860PurposeFluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertCytoBAS
ExpressionMammalianPromoterCMVAvailable SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
CamKII-ADAM23-pHluorin
Plasmid#185539PurposeExpresses ADAM23-pHluorin under the CamKII promoterDepositorAvailable SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-MITF
Plasmid#64888PurposeRetroviral expression of murine MITFDepositorInsertMITF (Mitf Mouse)
UseRetroviralAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV NucleoBAS
Plasmid#62861PurposeFluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertNucleoBAS
TagsSV40 NLSExpressionMammalianPromoterCMVAvailable SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSF-pMB1'-t7- YFP
Plasmid#186564PurposepMB1' ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
ExpressionBacterialPromoterT7 promoterAvailable SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAd5-Blue
Plasmid#174431PurposepAd5-Blue provides a platform for the rapid construction of recombinant and replication defective Adenovirus. This vector contains unique restriction enzyme sites to allow cloning of a gene.DepositorInsertβ-galactosidase α gene fragment
UseAdenoviralExpressionMammalianPromoterCMVAvailable SinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHRW-CRY2olig-VHH(GFP)
Plasmid#126023Purposeexpress RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogeneticsDepositorInsertCRY2 PHRolig-VHH(GFP)
TagsRFPExpressionInsectMutationCRY2 E490GPromoterHsp70Available SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV NucleoBAS N354K-I372V
Plasmid#63574PurposeFluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertNucleoBAS N354K-I372V
TagsSV40 NLSExpressionMammalianMutationN345K and I372VPromoterCMVAvailable SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HRW-CRY2-VHH(GFP)
Plasmid#126024Purposeexpress RFP-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHR-VHH(GFP)
TagsRFPExpressionInsectMutationWTPromoterHsp70Available SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Sox10
Plasmid#64890PurposeRetroviral expression of murine Sox10DepositorInsertsox10 (Sox10 Mouse)
UseRetroviralAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HFW-CRY2-VHH(GFP)
Plasmid#126027Purposeexpress Flag-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHR-VHH(GFP)
TagsFlagExpressionInsectMutationWTPromoterHsp70Available SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-tac- YFP
Plasmid#186570Purposep15A ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
ExpressionBacterialPromotertac promoterAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only