We narrowed to 23,974 results for: nes

1. pPHAGE_DLD-H450A-C-TAP

   Plasmid

   #83478

   Purpose

   Mammalian expression of the dehydrogenase-deficient mutant DLD-H450A.

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   DLD (DLD Human)
   

   Use

   Lentiviral

   Tags

   TAP

   Expression

   Mammalian

   Mutation

   H450A

   Promoter

   CMV

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPHAGE_DLD-S456A-C-TAP

   Plasmid

   #83479

   Purpose

   Mammalian expression of the proteolysis-deficient mutant DLD-S456A.

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   DLD (DLD Human)
   

   Use

   Lentiviral

   Tags

   TAP

   Expression

   Mammalian

   Mutation

   S456A

   Promoter

   CMV

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLD-puro-Cc-PRDX5WT-VA

   Plasmid

   #98688

   Purpose

   Lentiviral expression of human PRDX5-WT in mammalian cells

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   PRDX5 (PRDX5 Human)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLD-puro-Cc-CR-PARK7WT-VA

   Plasmid

   #115187

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLD-puro-Cc-CR-PARK7V51G-VA

   Plasmid

   #115188

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7V51G into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7V51G (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLD-puro-Cc-CR-PARK7C53A-VA

   Plasmid

   #115189

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7C53A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7C53A (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLD-puro-Cc-CR-PARK7H126A-VA

   Plasmid

   #115190

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7H126A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7H126A (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLD-puro-Cc-CR-PARK7E163K-VA

   Plasmid

   #115191

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7E163K into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7E163K (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLD-puro-Cc-PRDX5K85R-VA

   Plasmid

   #98691

   Purpose

   Lentiviral expression of human PRDX5-K85R in mammalian cells

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   PRDX5 (PRDX5 Human)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   K85R

   Promoter

   CMV

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLD-puro-Cc-PRDX5E80K-VA

    Plasmid

    #98689

    Purpose

    Lentiviral expression of human PRDX5-E80K in mammalian cells

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    PRDX5 (PRDX5 Human)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    E80K

    Promoter

    CMV

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLD-puro-Cc-PRDX5K83R-VA

    Plasmid

    #98690

    Purpose

    Lentiviral expression of human PRDX5-K83R in mammalian cells

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    PRDX5 (PRDX5 Human)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    K83R

    Promoter

    CMV

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLSC-5

    Plasmid

    #62889

    Purpose

    Lenti vector for expression of inducible split Cas9

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Inserts

    SpCas9(574-1368)
    SpCas9(2-573)
    

    Use

    CRISPR and Lentiviral

    Tags

    FFBP12, FRB, NES PTK2, NLS SV40, and P2A

    Expression

    Mammalian

    Mutation

    *see comment below

    Promoter

    EFS

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Cyto-GCaMP3

    Plasmid

    #64853

    Purpose

    Cytosol-targeted version of the Ca2+ indicator GCaMP3.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    GCaMP3
    

    Use

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

    Plasmid

    #196829

    Purpose

    Express dpdpCas13b with AAAA mutation and Clover

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    dpspCas13b(AAAA)
    

    Use

    Tags

    3XFLAG and NES

    Expression

    Mammalian

    Mutation

    K367A, D369A and K370A

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTL

    Plasmid

    #203176

    Purpose

    Centromeric yeast expression vector, leucine selection

    Depositor

    William Sessa

    Article

    Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)-Cyto-CaNARi

    Plasmid

    #64849

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Use

    Tags

    Cerulean, Nuclear export signal (NES), and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. MCP-ADAR2-DD

    Plasmid

    #170124

    Purpose

    Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MCP-ADAR2-DD-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a core promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQP-NiRISB

    Plasmid

    #138977

    Purpose

    Light-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAV0479

    Plasmid

    #133499

    Purpose

    Expression of sfGFP excluded from the nucleus with two NES signals

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pHis5(StuI)-p(tdh1)-NESwis1-sfGFP-NESmia1-natMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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