We narrowed to 27,649 results for: CAT

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pORANGE GFP-Actb KI #2

Plasmid

#139666

Purpose

Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

Plasmid

#103865

Purpose

Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

PspCas13b

Use

CRISPR and Lentiviral

Tags

3xHA and HIV NES

Expression

Mammalian

Mutation

Contains the H133A and H1058A mutations which ina…

Promoter

EF1a

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-dCasRx-ALKBH5-PURO

Plasmid

#175582

Purpose

Expresses dCasRx-ALKBH5 in mammalian cell

Depositor

Qi Xie

Article

Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

Insert

dCasRx-ALKBH5

Use

CRISPR and Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

RfxCas13d R239A/H244A/R858A/H863A

Promoter

pMSCV

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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AAV GFP

Plasmid

#49055

Purpose

adeno-associated virus (AAV) encoding a green fluorescent protein

Depositor

Fred Gage

Article

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-KO

Plasmid

#17603

Purpose

Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

KO: all lysines mutated to arginines

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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TMEM106B-EGFP

Plasmid

#210366

Purpose

Expresses TMEM106B fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TMEM106B (TMEM106B Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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MSGV1-1D3-28Z.1-3 mut

Plasmid

#107227

Purpose

Anti-mouse CD19 CAR with CD3 ITAMs mutated

Depositor

James Kochenderfer, Steven Rosenberg

Article

Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

Insert

1D3-28Z. 1-3 mut

Use

Retroviral

Tags

None

Mutation

None

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

Plasmid

#196703

Purpose

Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 D210N

Expression

Mammalian

Mutation

D210N, catalytically inactive

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo

Plasmid

#139449

Purpose

Lentiviral vector with gRNA scaffold and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

no sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7-mGFP

Plasmid

#62629

Purpose

mammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Tags

mEGFP inserted in M3-M4 loop

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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DUSP6

Plasmid

#40772

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

Insert

DUSP6 (DUSP6 Human)

Tags

6His and TEV

Expression

Bacterial

Mutation

Starting Met removed

Promoter

T7

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3/mStayGold(c4)=UtrCH

Plasmid

#212020

Purpose

For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

UtrCH

Tags

mStayGold(c4)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-B2 (GB3374)

Plasmid

#160636

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)

Depositor

Juan Antonio García, Fabio Pasin

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPN460

Plasmid

#137878

Purpose

PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRa;mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

POGZ (POGZ Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pXL010-Wnt dual (GFP-Fire) reporter

Plasmid

#40588

Depositor

Sean Palecek

Article

Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

Insert

Wnt reporter sequences

Use

Lentiviral

Expression

Mammalian

Promoter

Wnt promoter

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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hMEF2A WT

Plasmid

#118354

Purpose

This plasmid expresses human MEF2A-1 WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hMEF2A (MEF2A Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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MSGV-1D3-28Z All ITAMs intact

Plasmid

#107226

Purpose

Anti-mouse CD19 CAR with CD3 ITAMs intact

Depositor

James Kochenderfer, Steven Rosenberg

Article

Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

Insert

1D3-28Z All ITAMs intact

Use

Retroviral

Tags

None

Mutation

None

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag mHMGB2

Plasmid

#31610

Depositor

Yasuhiko Kawakami

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

Insert

HMGB2 (Hmgb2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
AAVS1-Puro CAG FUCCI

Plasmid

#136934

Purpose

donor plasmid for constitutive FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Use

Synthetic Biology

Tags

Clover and mKO2

Expression

Mammalian

Promoter

CAG

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pT7C-DNAJA1

Plasmid

#177655

Purpose

Recombinant protein synthesis of human DNAJA1

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

DNAJA1 (DNAJA1 Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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