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FLAG-Pol-II 15

Plasmid

#35177

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains the C-terminal repeats 16-52

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
CAG::PSAMQ79G:GlyR-IRES-GFP

Plasmid

#32482

Purpose

Expresses PSAM-GlyR (Q79G) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G-GlyR

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3 10 mut B-Myb

Plasmid

#25966

Depositor

Rob Lewis

Article

Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

Insert

B-Myb (Mybl2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed threonines 267, 408, 497, 519, 522 and 52…

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pSBDEST.H

Plasmid

#79464

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Hygromycin selection

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA1.1

Plasmid

#64116

Purpose

Vector for expression of St1 sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA1.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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PGEX 4T-1-PPARgamma2

Plasmid

#78773

Purpose

To purify GST-tagged recombinant protein

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPAR_2

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
R-R334W-(Kin-7)

Plasmid

#28178

Depositor

Bernard Schimmer

Article

Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

Insert

Prkar1a (PRKAR1A Mouse)

Expression

Mammalian

Mutation

R334W

Available Since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pDiySpike1

Plasmid

#136948

Purpose

Plasmid for the In Vitro transcription of DiySpike1

Depositor

Rickard Sandberg

Article

Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

Insert

DiySpike1

Use

Synthetic Biology

Promoter

T7

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCMB-AUTr

Plasmid

#61205

Purpose

Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LC3(ATG8)

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCY 3090-07

Plasmid

#36232

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

Streptoalloteichus hindustanus bleomycin (Sh ble)

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-mCherry-Zeocin

Available Since

May 23, 2012

Availability

Academic Institutions and Nonprofits only

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pRATIO1212

Plasmid

#141299

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG Enthoprotin Full Length

Plasmid

#47419

Depositor

Peter McPherson

Article

Wasiak et al J Cell Biol. 2002 Sep 2;158(5):855-62. d

Insert

Enthoprotin

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pICSL1P2_crU626

Plasmid

#191777

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P7_crU626

Plasmid

#191782

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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nonphosphorylatable Mis12-targeted Aurora B FRET sensor

Plasmid

#45229

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

non phosphorylatable Mis12-targeted Aurora B FRET sensor

Expression

Mammalian

Mutation

T-A mutation in substrate sequence

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II CC

Plasmid

#35180

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains C-terminal domain repeats (1-22)X2

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
786 pCSmAKT2Acherry

Plasmid

#31167

Depositor

Nathan Lawson

Article

Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

Insert

Akt

Use

Expression vector

Tags

Myr and mCherry

Mutation

membrane tethered Akt

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pDD399

Plasmid

#91821

Purpose

AID CT-tag linker donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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YN2_1_IL50_HOlocus

Plasmid

#194426

Purpose

Expresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

Cas9

Expression

Yeast

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pICSL1P6_crU626

Plasmid

#191781

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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