We narrowed to 25,845 results for: lars

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3'-3xFLAG minimal CMV pCDNA5

Plasmid

#212045

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FLT3

Plasmid

#183293

Purpose

All-in-One CRISPRko system with a guide RNA that targets FLT3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FLT3

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5myc Cdc42 N17

Plasmid

#15907

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Cdc42 (placental isoform) (CDC42 Human)

Tags

myc

Expression

Mammalian

Mutation

T17N

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRK5-mFzd6-1D4

Plasmid

#42268

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 6 (Fzd6 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
mEmerald-CENPB-N-22

Plasmid

#54037

Purpose

Localization: Nucleus/Centromeres, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

CENPB (CENPB Human)

Tags

mEmerald

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCAT.015

Plasmid

#119558

Purpose

Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_CSF1R

Plasmid

#183277

Purpose

All-in-One CRISPRko system with a guide RNA that targets CSF1R gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CSF1R

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJAG98-mTurquoise2-Nuf2

Plasmid

#80760

Purpose

Express Nuf2 N-terminally labeled with mTurquoise2

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

Nuf2 (NUF2 Human)

Use

Retroviral

Tags

mTurquoise2

Expression

Mammalian

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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PGL3-MK17

Plasmid

#21486

Depositor

Shoumo Bhattacharya

Article

Leung et al Genomics. 1999 Nov 1. 61(3):307-13.

Insert

Cited2 Promoter (CITED2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 23, 2009

Availability

Academic Institutions and Nonprofits only
pAC-PHYT

Plasmid

#53300

Purpose

Contains Erwinia herbicola Eho10 (Pantoea agglomerans) crtE and crtB genes, and thereby produces phytoene in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

Insert

crtE, crtB

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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Tt-NylC_pET28a

Plasmid

#215431

Purpose

Expression construct for Tt-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

Tt-NylC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-NHERF1-PDZ2

Plasmid

#28296

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF1 (NHERF1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains aa 148-298 (deletion of the first PDZ do…

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pB80-iLID-mCherry-RAB5

Plasmid

#174624

Purpose

Optogenetic coupling to early endosomes (RAB5) via iLID

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

iLID-mCherry-RAB5 (RAB5A Human, Synthetic)

Tags

iLID-mCherry

Expression

Mammalian

Mutation

mCherry: Met1Del; RAB5: Met1Del

Promoter

Chicken beta-actin

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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EK0302 SFFV-mCherry-SG(Bdnf.s2)SG-EGFP (FLP-IN)

Plasmid

#191179

Purpose

Negative control sensor in human cells.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s2:EGFP (negative control)

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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mStrawberry-Vimentin-7

Plasmid

#55945

Purpose

Localization: Intermediate Filaments, Excitation: 574, Emission: 596

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Vimentin (VIM Human)

Tags

mStrawberry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_synRobo

Plasmid

#188340

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Robo1-ECD, a Robo1-TMD, a Robo1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Robo1-ECD_Robo1-TMD_Robo1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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3XFlag DVL2 (∂PY)

Plasmid

#24808

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

DVL2 (DVL2 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of the PY motif: PPPY

Available Since

June 22, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pSB700 HL1gR4

Plasmid

#124450

Purpose

Expresses gRNA HL1 gR4 in the pSB700 backbone

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

HL1 gR4

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJEC581

Plasmid

#174369

Purpose

RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

RFP with PAM rich sequence upstream promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK102

Plasmid

#110574

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

T7 promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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