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AiP12267 - pAAV-AiE0494m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2267)

Plasmid

#223979

Purpose

AiE0494m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical cell types (note CB purkinje cells)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG-miniCGBE1

Plasmid

#198538

Purpose

Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-miniCGBE1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NANOS3_cterm

Plasmid

#222904

Purpose

Cas9/sgRNA plasmid for targeting NANOS3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NANOS3 sgRNA (NANOS3 Synthetic, Human)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry(Y70A)

Plasmid

#209703

Purpose

Mammalian expression of mCherry with Y70A mutation

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

mCherry(Y70A)

Expression

Mammalian

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1213

Plasmid

#144278

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

EVX2 (EVX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3327

Plasmid

#144803

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

LHX6 (LHX6 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-HsNot9_19-285_AF

Plasmid

#148733

Purpose

Bacterial Expression of HsNot9_19-285

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9_19-285 (CNOT9 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1356-1581_V

Plasmid

#147779

Purpose

Bacterial Expression of HsNot1iso1_1356-1581

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1356-1581 (CNOT1 Human)

Expression

Bacterial

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMC3-TPK2

Plasmid

#176180

Purpose

yeast MoClo level-0 part plasmid type 3 containing TPK2 gene

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

TPK2 (TPK2 Budding Yeast)

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IGF1R-F1057L

Plasmid

#116414

Purpose

Lentiviral expression of IGF1R F1057L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

IGF1R (IGF1R Human)

Use

Lentiviral

Mutation

F1057L

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCardinal-CAXX

Plasmid

#184451

Purpose

Plasma membrane-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-CAAX

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9-ABE-Umax-rest1

Plasmid

#222144

Purpose

Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

Plasmid

#113864

Purpose

Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

PTCH1 (hedgehog dependent promoter)
mTurquoise2

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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CAGGS-MCS-NLS-3XFLAG-V5

Plasmid

#196383

Purpose

Empty backbone vector to insert cDNA for strong expression in mammalian cells

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Type

Empty backbone

Tags

3xFLAG and V5

Expression

Bacterial and Mammalian

Promoter

CAGGs

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pNKX2-5-T2A-GFP-PGK-Puro

Plasmid

#203607

Purpose

Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

Depositor

Matthew Birket

Article

Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

Insert

NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)

Use

Human targeting

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-hTFIIB

Plasmid

#171084

Purpose

Expresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIB

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

TF2B (GTF2B Human)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7

Plasmid

#214138

Purpose

Insect cell expression of human 20S CP alpha-subunits

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA1 (PSMA1 Human)
PSMA2 (PSMA2 Human)
PSMA3 (PSMA3 Human)
PSMA4 (PSMA4 Human)
PSMA5 (PSMA5 Human)
PSMA6 (PSMA6 Human)
PSMA7 (PSMA7 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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IGF1R_pLX307

Plasmid

#98344

Purpose

Lentiviral expression of IGF1R

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

IGF1R (IGF1R Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-hALIX

Plasmid

#21504

Depositor

James Hurley

Article

Lee et al Science. 2008 Oct 24. 322(5901):576-80.

Insert

ALIX (PDCD6IP Human)

Tags

Cherry

Expression

Bacterial

Mutation

none

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pET23a-His-TEV-HaloTag-linker-SpyCatcher3

Plasmid

#235150

Purpose

Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

HaloTag linked to SpyCatcher3

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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