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pCDNAHygro(+)LAMP2B

Plasmid

#86029

Purpose

Expresses LAMP2B in Mammalian cells

Depositor

Janice Blum

Article

Zhou et al Immunity. 2005 May;22(5):571-81.

Insert

Lysosome-associated membrane protein 2b (LAMP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRN12

Plasmid

#84456

Purpose

shuttle plasmid for sarA P1-mAmetrine expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mAmetrine

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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T-2A-EGFP-PGK-Puro

Plasmid

#83344

Purpose

Donor vector to knocin EGFP into human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

Human Brachyury knockin homology arms (TBXT Human)

Tags

2A-EGFP

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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stathmin-GFP

Plasmid

#86782

Purpose

Mammalian expression of stathmin-GFP

Depositor

Lynne Cassimeris

Article

Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

Insert

stathmin (STMN1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-ArchT-WPREpA

Plasmid

#84472

Purpose

pAAV-RAM-ArchT

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

ArchT

Use

AAV

Tags

EGFP

Promoter

TRE

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAG303-Gal-GR100

Plasmid

#84908

Purpose

induce expression of dipeptide repeat GR in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

GR100 (C9orf72 Synthetic)

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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Hs.NRAS

Plasmid

#83173

Purpose

Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

NRAS (NRAS Human)

Use

Gateway Cloning

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pKA-142

Plasmid

#79835

Purpose

Expresses PpsR2-mVenus-CAAX under CMVd1 promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

PpsR2-mVenus-CAAX

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMVd1

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB515

Plasmid

#74857

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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Sumo3 PolQM1

Plasmid

#78462

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; Q2513R (please see depositor commen…

Promoter

T7

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pTag-BFP-C-h-Rab5a-c-Myc

Plasmid

#79801

Purpose

Express TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab5a (RAB5A Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pMAL-c4X

Plasmid

#75288

Purpose

Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available Since

July 19, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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Synapsin sHRPb-NLG in FSW lentiviral vector

Plasmid

#73148

Purpose

Small fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPb-NLG (Nlgn1 Synthetic, Mouse)

Use

Lentiviral

Tags

V5 tag

Expression

Mammalian

Promoter

Synapsin

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-EGFP

Plasmid

#73461

Purpose

Expresses EGFP for monosynaptic circuit mapping

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

EGFP

Use

Neurotropic virus

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-ninein

Plasmid

#73519

Purpose

Encodes mouse ninein tagged with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
pMXspuro-GFP-NBR1

Plasmid

#74202

Purpose

Retroviral expression of GFP-NBR1

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

neighbor of BRCA1 gene 1 (NBR1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only
pDD317 (Pmyo-2::GFP + SEC)

Plasmid

#73344

Purpose

SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

Insert

Pmyo-2::GFP + SEC

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Promoter

myo-2

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tet1 CD

Plasmid

#70129

Purpose

Express flag-tagged mouse Tet1 catalytic domain.

Depositor

Yi Zhang

Article

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET1 catalytic domain.

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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DH-BB(5'SIN;TE12ORF)

Plasmid

#72309

Purpose

Defective Helper to produce neurotropic, propagation-incompetent Sindbis virus

Depositor

Anthony Zador

Insert

DH-BB(5'SIN;TE12ORF)

Use

Sindbis virus helper construct

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pNUT N6His hTFNG

Plasmid

#67240

Purpose

Expresses N-His tagged nonglycosylated human serum transferrin in mammalian cells

Depositor

Ross MacGillivray, Anne Mason

Article

Mason et al Biochemistry. 2002 Jul 30;41(30):9448-54

Insert

human serum transferrin (TF Human)

Tags

N-terminal signal peptide, 4 aa link, 6 His, Fact…

Expression

Mammalian

Mutation

Asn413 Asp, Asn611Asp

Promoter

SV40

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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