159,557 results

1. pAAV-Ef1a-fDIO-JRGECO1a

   Plasmid

   #128317

   Purpose

   Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

   Depositor

   Patricia Jensen

   Has Service

   AAV1

   Insert

   NES-JRGECO1a
   

   Use

   AAV

   Tags

   His Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LEPZ

   Plasmid

   #111161

   Purpose

   miR-E (miR-30 variant)-based RNAi with ZsGreen reporter

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX_TRE3G_DD::mCherry

   Plasmid

   #108679

   Purpose

   Tunable system for controlled gene expression and protein stability in mammalian cells

   Depositor

   Lucia Marucci

   Article

   Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

   Insert

   Destabilizing Domain (DD) mCherry fusion protein
   

   Use

   Lentiviral

   Tags

   DD::mCherry fusion protein

   Expression

   Mammalian

   Mutation

   Promoter

   Doxycycline inducible

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-HA-USP5

   Plasmid

   #22590

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP5 (USP5 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF0884

   Plasmid

   #142702

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HOXB4 (HOXB4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUC01

   Plasmid

   #177204

   Purpose

   chromogenic reporter for gene knockout in Streptomyces

   Depositor

   Wenjun Guan

   Article

   Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

   Insert

   SaindC
   

   Use

   Synthetic Biology

   Tags

   NONE

   Expression

   Bacterial

   Mutation

   NONE

   Promoter

   TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_SLC7A5

   Plasmid

   #132197

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC7A5 (SLC7A5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pENTR-LUC (w158-1)

   Plasmid

   #17473

   Purpose

   Gateway entry vector, Firefly Luciferase

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Firefly Luciferase
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGW-GOI-TurboID

   Plasmid

   #209378

   Purpose

   Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTECH-chPylRS(IPYE)

    Plasmid

    #99222

    Purpose

    Expression plasmid encoding chPylRS(IPYE) and pylT

    Depositor

    Dieter Söll

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp chPyl(IPYE)RS; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEXP5-NT/6xHis mCherry F30 2xdBroccoli

    Plasmid

    #169233

    Purpose

    Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

    Depositor

    Dora Tang

    Article

    Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

    Insert

    6xHis mCherry F30-2xdBroccoli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pet30b-5M SrtA

    Plasmid

    #51140

    Purpose

    expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

    Depositor

    Hidde Ploegh

    Article

    Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

    Insert

    S.aureus SrtA 5M
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1a-BirA-V5-neo

    Plasmid

    #100548

    Purpose

    Expresses C-terminal V5-tagged BirA biotin ligase

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    C-terminal V5-tagged BirA
    

    Use

    Tags

    V5, His

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-PIK3CA-H1047R

    Plasmid

    #116500

    Purpose

    Lentiviral expression of PIK3CA H1047R

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    H1047R

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG_mRuby2_smFP FLAG

    Plasmid

    #59760

    Purpose

    encodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    mRuby2_smFP_FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. A*02:02:01:01

    Plasmid

    #203277

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*02:02:01:01 (HLA-A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBABE-SSeCKs

    Plasmid

    #106908

    Purpose

    full-length murine SSeCKs/AKAP12/Gravin in retroviral vector

    Depositor

    Heidi Hehnly

    Article

    Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

    Insert

    SSeCKs (Akap12 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    gag

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMEH373 dTomato 3x high affinity MS2 loops

    Plasmid

    #168221

    Purpose

    Mammalian expression of dTomato with 3 high affinity MS2 loops

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    dTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AIO-Puro

    Plasmid

    #74630

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Puromycin resistant marker

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. KRphi-mRFP

    Plasmid

    #17276

    Depositor

    Sergio Grinstein

    Article

    Yeung et al Science. 2006 Jul 21. 313(5785):347-51.

    Insert

    KR phi
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2008

    Availability

    Academic Institutions and Nonprofits only