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ePPEplus

Plasmid

#205241

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP-G3BP1(1)

Plasmid

#136058

Purpose

G3BP1 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (GTAGTCCCCTGCTGGTCGGGC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Mfn2-Myc

Plasmid

#23213

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn2 (Mfn2 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only

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pRN114

Plasmid

#127221

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, Wus, AtSTM

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pRN227

Plasmid

#127222

Purpose

BeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Cas9, WUS2, AtSTM, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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PylT(UAG) PylS mCherry-P2A-eGFP(150TAG)

Plasmid

#174526

Purpose

Dual-fluorescence reporter for quantifying PylT(UAG) decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Insert

PylS

Tags

Flag

Expression

Mammalian

Promoter

EF-1α

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA6 asyn E46K

Plasmid

#105730

Purpose

Mammalian expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

Insert

alpha-synuclein E46K (SNCA Human)

Expression

Mammalian

Mutation

E46K

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_CD9-SBP-EGFP

Plasmid

#222328

Purpose

Synchronize the trafficking of CD9 from the ER.

Depositor

Franck Perez

Article

Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

Insert

Streptavidin-KDEL and CD9 fused to SBP-EGFP (CD9 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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DRD2-3xHA/pCDNA3.1

Plasmid

#196940

Purpose

Plasmid expressing an antigen targeting the D2 dopamine receptor

Depositor

James Trimmer

Insert

DRD2 (DRD2 Human)

Tags

3xHA

Expression

Mammalian

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSIN-TRE-EB3N-VVDfast-mCl3-iLID

Plasmid

#190169

Purpose

Doxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

EB3N-VVDfast-mClover3-iLID

Use

Lentiviral

Tags

-VVDfast-mClover3-iLID

Mutation

1-189 aa from EB3

Promoter

TRE

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_EGFP_NbALFA

Plasmid

#136626

Purpose

Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-NbALFA

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pIVT-mGL

Plasmid

#232768

Purpose

IVT template for making modRNA encoding mGreenLantern

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mGreenLantern

Use

In vitro transcription

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

Plasmid

#211471

Purpose

lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

Cas9_T2A_mNeonGreen_P2A_BlasticidinR

Use

CRISPR and Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

hU6, EF-1a

Available Since

March 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHR CD19-FcGamma CAR

Plasmid

#113014

Purpose

for production of lentivirus encoding the CD19 CAR-Pfcgamma

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P intracellular GFP (CD19 Human, Synthetic, Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 wt

Plasmid

#16434

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 (TP53 Human)

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pAID5.3-C

Plasmid

#145813

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Tags

mAID

Expression

Mammalian

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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SNAP-Rab5

Plasmid

#169069

Purpose

Express human Rab5b in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab5B (RAB5B Human)

Tags

SNAP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIB-H2B-mCherry

Plasmid

#164244

Purpose

retroviral plasmid for H2B-mCherry

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

H2B

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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CLYBL hOPM

Plasmid

#112499

Purpose

Homologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in CLYBL locus

Depositor

Bruce Conklin

Insert

OTX2, PAX6, MITF (PAX6, MITF, OTX2 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ER-mRFP

Plasmid

#231176

Purpose

Expresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, in mammalian cells

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

ER-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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