We narrowed to 9,389 results for: Coli

1. pDBD-M

   Plasmid

   #50572

   Purpose

   Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571

   Depositor

   Alexander Varshavsky

   Article

   Turner et al Science. 2000 Sep 22;289(5487):2117-20.

   Insert

   DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCRISPR

   Plasmid

   #113252

   Purpose

   pUC19 backbone with E. coli CRISPR array

   Depositor

   Jennifer Doudna

   Article

   Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

   Insert

   E. coli CRISPR array
   

   Use

   Integration substrate

   Tags

   Expression

   Mutation

   Promoter

   NA

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET29b-ClbP

   Plasmid

   #48244

   Purpose

   Untagged, full-length protein for in vivo peptidase assays

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbP
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFF714

   Plasmid

   #61449

   Purpose

   IPTG-inducible SCRIBE_galK(OFF)

   Depositor

   Timothy Lu

   Article

   Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

   Insert

   SCRIBE_galK(OFF)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pLacO

   Available since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDBD-R

   Plasmid

   #50571

   Purpose

   Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572

   Depositor

   Alexander Varshavsky

   Article

   Turner et al Science. 2000 Sep 22;289(5487):2117-20.

   Insert

   DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   M1R (B-gal)

   Promoter

   Gal1

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1 FLIPglu-170nDelta13V

   Plasmid

   #18018

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   FLIPglu F16F Delta13
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   WT binding protein (no F16A)

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUS200

   Plasmid

   #17788

   Depositor

   Stephen Sligar

   Article

   Unger et al J Biol Chem. 1986 Jan 25. 261(3):1158-63

   Insert

   Cytochrome P450cam
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pClodANL-Cherry

   Plasmid

   #107870

   Purpose

   Bacterial expression of cherry-NanoLuc

   Depositor

   Sanford Simon

   Article

   Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

   Insert

   Cherry (red fluorescent protein)
   

   Use

   Luciferase

   Tags

   NanoLuc

   Expression

   Bacterial

   Mutation

   Promoter

   pro D

   Available since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSG 627

   Plasmid

   #63872

   Purpose

   Measurements of translational accuracy

   Depositor

   Albert Dahlberg

   Article

   O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

   Insert

   lacZ (lacZ Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   UAA near 5' end

   Promoter

   PtacI

   Available since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pFA6a-hphMX6-P81nmt1-3FLAG

    Plasmid

    #19350

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    hphMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDL12

    Plasmid

    #17834

    Depositor

    Jay Keasling

    Article

    Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

    Insert

    gpdA promoter, lacZ, argB,
    

    Use

    Synthetic Biology; Aspergillus nidulans expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBbE2k-pTet-gRNAwcaF159

    Plasmid

    #122259

    Purpose

    aTc inducible gRNA expression plasmid

    Depositor

    Chueh Loo Poh

    Article

    Zhang et al Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.

    Insert

    gRNAwcaF159
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSG 853

    Plasmid

    #63873

    Purpose

    Measurements of translational accuracy

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

    Insert

    lacZ (lacZ Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    UAA near 5' end

    Promoter

    PtacI

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pSG 163

    Plasmid

    #63871

    Purpose

    Measurements of translational accuracy

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

    Insert

    lacZ (lacZ Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    UAG near 5' end

    Promoter

    PtacI

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pRSET FLIPmal

    Plasmid

    #14478

    Depositor

    Wolf Frommer

    Article

    Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

    Insert

    FLIPmal
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSelect-2

    Plasmid

    #190242

    Purpose

    Encodes lacI-targeting gRNA for positive selection of dCas9 activity.

    Depositor

    Marc Ostermeier

    Article

    Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

    Insert

    LacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-hphMX6-P41nmt1-3FLAG

    Plasmid

    #19349

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    hphMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKP1141

    Plasmid

    #160811

    Purpose

    Expression of TonB protein

    Depositor

    Kathleen Postle

    Article

    Gresock et al Front Microbiol. 2011 Oct 12;2:206. doi:

    Insert

    tonB (tonB Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFLIPmal-40uDelta1Venus

    Plasmid

    #18854

    Depositor

    Wolf Frommer

    Article

    Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

    Insert

    pFLIPmal-40uDelta1V
    

    Use

    Tags

    CFP and Venus

    Expression

    Bacterial

    Mutation

    N227A

    Promoter

    Available since

    Oct. 23, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pRB30

    Plasmid

    #165480

    Purpose

    pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal Flag and His

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    TEV-FLAG-10x His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFA6a-hphMX6-P3nmt1-3FLAG

    Plasmid

    #19348

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    hphMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMON-HsNMT2

    Plasmid

    #67476

    Purpose

    Expression of human N-myristoyltransferase 2 for N-myristoylation of ARF family proteins in bacteria

    Depositor

    Richard Kahn

    Article

    Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

    Insert

    HsNMT2 (NMT2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

23. circRAJ31

    Plasmid

    #69926

    Purpose

    Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator.

    Depositor

    Alfonso Jaramillo

    Article

    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

    Insert

    plTet-circRAJ31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PltetO1-2

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFF746

    Plasmid

    #61451

    Purpose

    aTc-inducible SCRIBE_galK(ON)

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    SCRIBE_galK(ON)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pTetO

    Available since

    March 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNK141k2

    Plasmid

    #156384

    Purpose

    Expresses orthogonal RBS mCarmine reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    mCarmine
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FLIPrbs F16A

    Plasmid

    #17864

    Depositor

    Wolf Frommer

    Article

    Lager et al FEBS Lett. 2003 Oct 9. 553(1-2):85-9.

    Insert

    FLIPrbs F16A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    F16A

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNK141f2

    Plasmid

    #156379

    Purpose

    Expresses orthogonal RBS Venus reporter

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    Venus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD24MevT

    Plasmid

    #17820

    Depositor

    Jay Keasling

    Article

    Pitera et al Metab Eng. 2007 Mar . 9(2):193-207.

    Insert

    MevT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRSET FLIPmal-25u

    Plasmid

    #14480

    Depositor

    Wolf Frommer

    Article

    Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

    Insert

    FLIPmal
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    W230A

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

30. pBbE7c-XAacxnT (JBEI-14118)

    Plasmid

    #100961

    Purpose

    XAacx operon in pBbE7c

    Depositor

    Jay Keasling

    Article

    Brown et al ACS Synth Biol. 2016 Dec 16;5(12):1485-1

    Insert

    XAacx operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTK203

    Plasmid

    #13536

    Depositor

    Wolf Frommer

    Article

    Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

    Insert

    FLIPW-TCTY
    

    Use

    Tags

    See map

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKB253

    Plasmid

    #170036

    Purpose

    Encodes LacI-controlled expression of surface-displayed K5L7 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    K5L7
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    May 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. No-repeats

    Plasmid

    #122487

    Purpose

    A control plasmid that is similar to PRExpress but lacks poly-PRE sequence. (GenBank: MF058599.1)

    Depositor

    Renato Paro

    Article

    Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

    Insert

    LacZ
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. FLIPrbs Q235A

    Plasmid

    #17862

    Depositor

    Wolf Frommer

    Article

    Lager et al FEBS Lett. 2003 Oct 9. 553(1-2):85-9.

    Insert

    FLIPrbs Q235A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    Q235A

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

35. pN23-LIC-His-FLAG

    Plasmid

    #165478

    Purpose

    pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    10xHis-FLAG-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFF828

    Plasmid

    #61456

    Purpose

    AHL-inducible msd_lacZ(ON)

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    msd_lacZ(ON)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLuxR

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSEDuet40

    Plasmid

    #46378

    Depositor

    Hideo Iwai

    Article

    Ellila et al FEBS Lett. 2011 Oct 10.

    Insert

    TvoVMA intein
    

    Use

    Tags

    GB1 and H6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMMDuet19

    Plasmid

    #45594

    Depositor

    Hideo Iwai

    Article

    Ellila et al FEBS Lett. 2011 Oct 10.

    Insert

    NpuDnaB mini intein
    

    Use

    Tags

    GB1 and H6-GB1

    Expression

    Bacterial

    Mutation

    removal of endonuclease domain

    Promoter

    T7

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FLIPrbs F15A

    Plasmid

    #17863

    Depositor

    Wolf Frommer

    Article

    Lager et al FEBS Lett. 2003 Oct 9. 553(1-2):85-9.

    Insert

    FLIPrbs F15A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    F15A

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

40. pDL11

    Plasmid

    #17832

    Depositor

    Jay Keasling

    Article

    Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

    Insert

    alcA promoter, lacZ, argB,
    

    Use

    Synthetic Biology; Aspergillus nidulans expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2008

    Availability

    Academic Institutions and Nonprofits only