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HSD17B11
Plasmid#39159PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHSD17B11 (HSD17B11 Human)
UseTagsHis TevExpressionBacterialMutationcodon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
EHMT1 (3HNA)
Plasmid#25305PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEHMT1_07 (EHMT1 Human)
UseTagsHisExpressionBacterialMutationC-terminal domain, residues 951-1235PromoterAvailable sinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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SepOTSι
Plasmid#68290PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS9/EFSep21, 2xtRNA-Sep-A37
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSλ
Plasmid#68292PurposePhosphoserine orthogonal translation system (preferred variant)DepositorInsertSepRS9/EFSep21, 4xtRNA-Sep-A37
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB-DEST (JDW 1205)
Plasmid#229821PurposeA PiggyBac destination vector compatible with gateway cloning system.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA041
Plasmid#215963PurposeFragmid fragment: (Pol 2 promoter) human core (short) EF1a promoterDepositorHas ServiceCloning Grade DNAInsertEFS_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
HSD17B10
Plasmid#39153PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHSD17B10 (HSD17B10 Human)
UseTagsHis TevExpressionBacterialMutationcodon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro H-Ras G12V E37G
Plasmid#12275DepositorInsertH-Ras G12V E37G (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationContains G12V activating mutation. The E37G mutat…PromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pWZL Blast H-Ras G12V E37G
Plasmid#12277DepositorInsertH-Ras G12V E37G (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationContains G12V activating mutation. The E37G mutat…PromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
EHMT1_exon 3_gRNA
Plasmid#228809PurposeCRISPR targeting of human EHMT1DepositorInsertgRNA targeting EHMT1 exon 3 (EHMT1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)
Plasmid#229837PurposeA Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.DepositorInsertLuciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2
Plasmid#216173PurposeExpress the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- FOXA.bs- ZEB2.locus
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMP-Ehmt1_2
Plasmid#36336DepositorInsertEhmt1 (EHMT1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-Ehmt1_4
Plasmid#36338DepositorInsertEhmt1 (EHMT1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-Ehmt1_1
Plasmid#36335DepositorInsertEhmt1 (EHMT1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xHA-MKK7a1-EE
Plasmid#47578PurposeBacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1DepositorInsertMKK7a1-EE (Map2k7 Mouse)
UseTags3xHA and GSTExpressionBacterialMutationResidues 73-420 of RefSeq sequence. Ser271 to Gl…PromoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pAWp40
Plasmid#157980PurposepFUGW-EFSp-BFP-{SbfI}DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMP-Ehmt1_3
Plasmid#36337DepositorInsertEhmt1 (EHMT1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xHA-MKK4-EE
Plasmid#47577PurposeBacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4DepositorInsertMKK4-EE (MAP2K4 Human)
UseTags3xHA and GSTExpressionBacterialMutationLacks the first 36 residues of the RefSeq protein…PromoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cas9_YTHDF2_sgRNA
Plasmid#186673PurposeYTHDF2 sgRNA plasmidDepositorInsertYTHDF2 KO sgRNA Plasmid (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX62
Plasmid#46854PurposeTOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCEDepositorInsertattB-Puro-2A and Hupki p53 G245S (TP53 Mouse, Human)
UseTagsExpressionMammalianMutationG245SPromoterAvailable sinceAug. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX68
Plasmid#46857PurposeOP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCEDepositorInsertattB-Puro-2A and Hupki p53 A138V (TP53 Mouse, Human)
UseTagsExpressionMammalianMutationp53 A138VPromoterAvailable sinceAug. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SCN1B-COMP5AP-AviTag-9xHis
Plasmid#157099PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertSCN1B (SCN1B Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO5-SgHottip-GFP-CRISPRa
Plasmid#134990PurposedCas9-mediated activation of HOTTIP in mammalian cellsDepositorInsertHOTTIP (HOTTIP Human)
UseLentiviralTagsGFPExpressionBacterial and MammalianMutationPromoterU6Available sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cas9_ANKRD1_sgRNA2
Plasmid#186669PurposePlasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.DepositorInsertANKRD1 KO sgRNA Plasmid (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO5-SgHottip-GFP-CRISPRi
Plasmid#134989PurposedCas9-mediated inactivation of HOTTIP in mammalian cellsDepositorInsertHOTTIP (HOTTIP Human)
UseLentiviralTagsGFPExpressionBacterial and MammalianMutationPromoterU6Available sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN1A-Variant-1-IRES-mScarlet
Plasmid#162278PurposeEukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN1A Variant 1, stabilized (SCN1A Human)
UseTagsIRES-mScarletExpressionMammalianMutationIVS intron inserted after bp2946. Silent codon c…PromoterCMVAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nav1.7 Na+ channel scFv [N68/6]
Plasmid#182094PurposeMammalian Expression of Nav1.7 Na+ channel scFv. Derived from hybridoma N68/6.DepositorInsertrecombinant mouse scFv targeting Nav1.7 Na+ channel (Homo sapiens) (SCN9A Mouse)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SCN1B-Fc(DAPA)-AviTag-6xHis
Plasmid#156534PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertSCN1B (SCN1B Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-Nav1.7 Na+ channel [N68/6R]
Plasmid#177566PurposeMammalian Expression Plasmid of anti-Nav1.7 Na+ channel (Human). Derived from hybridoma N68/6.DepositorInsertanti-Nav1.7 Na+ channel (Homo sapiens) recombinant mouse monoclonal antibody (SCN9A Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
GABA(A)R, gamma2 scFv [N452/81]
Plasmid#182088PurposeMammalian Expression of GABA(A)R, gamma2 scFv. Derived from hybridoma N452/81.DepositorInsertrecombinant mouse scFv targeting GABA(A)R, gamma2 (Homo sapiens) (GABRG2 Mouse)
UseTagsSortase, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-Nav1.7 Na+ channel [N68/6R-1]
Plasmid#206717PurposeMammalian Expression Plasmid of anti-Nav1.7 Na+ channel (Human). Derived from hybridoma N68/6-1.DepositorInsertanti-Nav1.7 Na+ channel (Homo sapiens) recombinant Mouse monoclonal antibody (SCN9A Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Nav1.7 Na+ channel [N68/6R-2b]
Plasmid#206674PurposeMammalian Expression Plasmid of anti-Nav1.7 Na+ channel (Human). Derived from hybridoma N68/6-2b.DepositorInsertanti-Nav1.7 Na+ channel (Homo sapiens) recombinant Mouse monoclonal antibody (SCN9A Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
GABA(A)R, Gamma2L/S scFv [N452/30]
Plasmid#206785PurposeMammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/30 scFv.DepositorInsertGABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
GABA(A)R, Gamma2L/S scFv [N452/73]
Plasmid#190555PurposeMammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/73.DepositorInsertGABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only
