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pLJD-KLF4-ER

Plasmid

#34596

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Retroviral

Tags

Myc epitope tag and estrogen receptor fusion

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pGL3-158/+19

Plasmid

#35541

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

none

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pGL3-110/+19

Plasmid

#35542

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

none

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pGL3-64/+19

Plasmid

#35543

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

none

Available since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only

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CMV-FFluc-GPD1L-3'UTR WT

Plasmid

#32488

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1L 3'UTR (Gpd1l Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
psiCHECK2-CCNA2 LONG 3'UTR

Plasmid

#29472

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

CCNA2 LONG 3'UTR (CCNA2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pCS252

Plasmid

#33207

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

MAP4K4 3'UTR (MAP4K4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-CGCG sites: aaCgCga, aaCgCgaa

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
ER-RspA-NFAST

Plasmid

#233595

Purpose

Expression of RspA-NFAST on the ER membrane

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-NFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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ER-long-RspA-NFAST

Plasmid

#233600

Purpose

Expression of RspA-NFAST on the ER membrane after a 55 aminoacid linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-NFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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OMM-short-RspA-NFAST

Plasmid

#233603

Purpose

Expression of RspA-NFAST on the outer mitochondrial membrane

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-NFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drN-hsAgo2

Plasmid

#221724

Purpose

Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for zebrafish Ago2 N-domain.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drN-hsAgo2 (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

Swap of N-domain for zebrafish Ago2 N-domain

Promoter

simian CMV IE94

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsN-drAgo2

Plasmid

#221717

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsN-drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

Swap of N-domain for human Ago2 N-domain

Promoter

simian CMV IE94

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

Plasmid

#199580

Purpose

Expresses GCaMP8f in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

His-DIO GCaMP8f

Use

AAV, CRISPR, and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

hsyn

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2EF-DY

Plasmid

#221723

Purpose

Expression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2EF-DY (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

E637D and F666Y in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-RepoMan-RATA

Plasmid

#184270

Purpose

Expresses shRNA resistant GFP-RepoMan RATA Mutant

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell division cycle associated 2 (CDCA2 Human)

Use

Tags

3xHA and GFP

Expression

Mammalian

Mutation

V383A, F395A

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-RepoMan-T394A

Plasmid

#204994

Purpose

Expresses shRNA resistant GFP-RepoMan T394A Mutant

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell devision cycle associated 2 (CDCA2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed Threonine 394 to Alanin

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Parkin(C431N)-A92mKO2

Plasmid

#213545

Purpose

Transient mammalian expresssion of ligase-dead C431N Parkin, internally tagged with mKO2

Depositor

Lani Wu, Steven Altschuler

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Tags

Expression

Mammalian

Mutation

C431N mutation in Parkin

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLv-EF1a-Parkin(K161N)-A92mKO2

Plasmid

#213554

Purpose

Lentiviral vector for stable expression of K161N Parkin, internally tagged with mKO2

Depositor

Lani Wu, Steven Altschuler

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K161N mutation in Parkin

Promoter

EF1a

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLv-EF1a-Parkin(R104A)-A92mKO2

Plasmid

#213556

Purpose

Lentiviral vector for stable expression of R104A Parkin, internally tagged with mKO2

Depositor

Lani Wu, Steven Altschuler

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

R104A mutation in Parkin

Promoter

EF1a

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLv-EF1a-Parkin(S65A)-A92mKO2

Plasmid

#213557

Purpose

Lentiviral vector for stable expression of S65A Parkin, internally tagged with mKO2

Depositor

Lani Wu, Steven Altschuler

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S65A mutation in Parkin

Promoter

EF1a

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge