We narrowed to 4,461 results for: erf

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CROP_C9_Puro_TOP1

Plasmid

#183323

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP1

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJK2020

Plasmid

#171932

Purpose

express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon

Depositor

Judith Kimble

Article

Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

Insert

Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR

Tags

superfolder GFP, mCherry

Expression

Worm

Promoter

-

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1A

Plasmid

#183337

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1A

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagRFP657-2A

Plasmid

#235537

Purpose

To express tagRFP657-2A

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Type

Empty backbone

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FGFR3

Plasmid

#183291

Purpose

All-in-One CRISPRko system with a guide RNA that targets FGFR3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FGFR3

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 WAVE 2

Plasmid

#67098

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

WAVE 2 (WASF2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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PT7-deGFP-MGapt

Plasmid

#67741

Purpose

T7 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T7term

Use

Synthetic Biology

Expression

Bacterial

Promoter

PT7

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-PDZD8HA_sh_resistant

Plasmid

#105006

Purpose

Mammalian Expression of PDZD8, sh resistant

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

PDZD8 (Pdzd8 Mouse)

Tags

HA

Expression

Mammalian

Mutation

The sequence recognized by shPDZD8 (TRCN000043105…

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1B

Plasmid

#183338

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1B

Use

Lentiviral

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TOP2A

Plasmid

#183324

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP2A gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP2A

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKERep

Plasmid

#211826

Purpose

Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pKESa

Plasmid

#211828

Purpose

Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hTXLNA-HA

Plasmid

#165472

Purpose

Human TXLNA mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

taxilin alpha (TXLNA Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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Tubulin-mhYFP

Plasmid

#186525

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

Tubulin

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

Plasmid

#133339

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18-H1 RNAi

Plasmid

#87355

Purpose

RNAi expression vector based on H1 promoter and pUC18 vector backbone

Depositor

Saroj Mathupala

Article

Mathupala et al Neurosurgery. 2004 Dec;55(6):1410-9; dis

Insert

H1 RNA Promoter from U87MG glioma cells

Use

RNAi; Shrna expression vector

Expression

Mammalian

Promoter

H1 RNA

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pNL4-3 envFS eGFP NoStarts

Plasmid

#101342

Purpose

All ATGs from the start of gag to NC mutated to ATC except the first which was mutated to ACG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

No methonines in Gag

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_TTGmiRc2

Plasmid

#25753

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Tags

GFP

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pEN_TmiR_Luc-A

Plasmid

#25760

Purpose

Entry vector withTRE promoter driving control Luciferase miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Gateway Cloning

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNAmreB

Plasmid

#149654

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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