We narrowed to 12,783 results for: NUC
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Plasmid#124035PurposeEncodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a human non-targeting sgRNA in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pEF1a-iRFP713_non targeting sgRNA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0465
Plasmid#124036PurposeEncodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a mScarlet-targeting sgRNA in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pEF1a-iRFP713_mScarlet sgRNA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0466
Plasmid#124037PurposeEncodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a tagBFP-targeting sgRNA in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pEF1a-iRFP713_tagBFP sgRNA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0468
Plasmid#124038PurposeEncodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of tagBFP- and mScarlet-targeting sgRNAs in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pEF1a-iRFP713_mScarletsgRNA_tagBFP sgRNA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag D16A
Plasmid#109031PurposeD16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-ScSEIPIN-Ndelta
Plasmid#96985PurposeExpress yeast SEIPIN gene in plants.DepositorInsertScSEIPIN (SEI1 Budding Yeast)
Tags6xHis and GFPExpressionPlantMutationN-terminal 39 nucleotides were deleted from yeast…Promoter35SAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc NES-mCE
Plasmid#82463PurposeExpression of myc epitope and murine mRNA Capping Enzyme with Terminal nuclear export signal in mammalian cellsDepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-3xFLAG-OB D166R
Plasmid#79061PurposeRetroviral expression of 3xFLAG tagged, nuclear localized TPP1-OB-RR folding domainDepositorInsertTPP1 (TPP1 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationAspartic acid 166 changed to ArgininePromoterCMVAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tg 2 kb Violet opsin GFP
Plasmid#72921Purposeviolet opsin promoter from zebra finch (Taeniopygia guttata) gDNA (nucleotides21,946 to 145, corresponding to chr1A_ran-dom:206453–208444 in taeGut1) driving GFPDepositorInsertviolet opsin promoter
Available SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tg 2 kb Violet opsin DsRed
Plasmid#72920Purposeviolet opsin promoter from zebra finch (Taeniopygia guttata) gDNA (nucleotides21,946 to 145, corresponding to chr1A_ran-dom:206453–208444 in taeGut1) driving DsRedDepositorInsertviolet opsin promoter
Available SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ac 1.8kb red opsin GFP.
Plasmid#72915Purposered opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving GFPDepositorInsertred opsin promoter
Available SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ac 1.8kb red opsin DsRed
Plasmid#72914Purposered opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving DsRedDepositorInsertred opsin promoter
Available SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
719-2µ-minHIS3
Plasmid#40610DepositorInsertHIS3 (HIS3 Budding Yeast, Synthetic)
TagsHAExpressionYeastMutationEvery codon of the coding sequence has been repla…PromoterTDH3Available SinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-mCherry-PKCsub-RLuc8N-NES
Plasmid#138213PurposeEncodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKCsub-RLuc8N-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBS-Hsp70-Cas9
Plasmid#46294PurposeA codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.DepositorInsertcodon optimized Cas9
UseCRISPRTags3x FlagExpressionInsectMutationSilent change from C to A at bp 834 of insertPromoterHsp70Available SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
DHB-mVenus
Plasmid#136461PurposeFluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2DepositorAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBBR1.cas9-NT
Plasmid#197871PurposeConstitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)DepositorInsertstrancCRNA
Cas9
crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
ExpressionBacterialAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-NLS-mRuby2
Plasmid#99130PurposeAn AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancerDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertNLS-mRuby2
UseAAVExpressionMammalianPromotermDLX5/6Available SinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLIX401 APE1 WT
Plasmid#227190PurposeInducible lentiviral expression of WT APE1DepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only