We narrowed to 66,633 results for: cell;
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Plasmid#171108PurposeHomology directed repair plasmid for generating HaloTag-EZH2 cellsDepositorAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only
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Talin1 head-mCherry
Plasmid#212005PurposeExpression of mouse talin1 head (1-490)-mCherry in mammalian cellsDepositorInsertMouse talin1 head domain (1-490) (Tln1 Mouse)
TagsmCherry fluorescent proteinExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-pegRNA_SRD5A3-EGFP
Plasmid#159781PurposeS. pyogenes pegRNA for C to A mutation at the SRD5A3 site of human cells using prime editingDepositorInsertspacer of pegRNA targeting SRD5A3 gene (SRD5A3 Human)
ExpressionMammalianAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-Y419F
Plasmid#140317PurposeExpresses Flag-tagged human Src-Y419F in mammalian cells for structure-function studiesDepositorAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-TMPRSS3
Plasmid#221298PurposeExpression of TMPRSS3 (pLxIS) in mammalian cells by retroviral transductionDepositorAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF6 VPS51-V5-6His
Plasmid#175502PurposeV5 tagged VPS51 is expressed in mammalian cellsDepositorInsertVPS51 (VPS51 Human)
TagsV5ExpressionMammalianMutationA661V- please see depositor commentsAvailable SinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-EGFP-NEMO-D304N
Plasmid#187898PurposeMammalian cell expression of D304N mutation in NEMODepositorInsertHA-EGFP-NEMO-D304N (Ikbkg Mouse)
TagsEGFP and HAExpressionMammalianMutationD304NPromoterCMVAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-N3FLAG-MYB-FL
Plasmid#105609Purposetransient express full length MYB with 3*FLAG tag at N terminal in HEK 293T cellsDepositorAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMP926
Plasmid#135001Purposem6A Reader-Writer. Propagates Gm6ATC along GATC arrays as well as across cell generations. pUBC-DpnI(aa146-254)-3xFLAG-Dam(R95A) pGK-HygroRDepositorInsertpUBC-DpnI(aa146-254)-3XFLAG-Dam(R95A) pGK-HygroR
UseLentiviral and Synthetic BiologyTags3xFLAG, Dam(R95A), and DpnI(aa146-254)ExpressionMammalianMutationDpnI domain truncated to aa146-254. Dam protein m…Available SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3−3xFLAG−AGO2(S387A)
Plasmid#220462PurposeExpress S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cellsDepositorInsertHuman AGO2 (AGO2 Human)
Tags3xFLAGExpressionMammalianMutationSerine 387 to alaninePromoterCMVAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3−3xFLAG−AGO2(S387D)
Plasmid#220463PurposeExpress S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cellsDepositorInsertHuman AGO2 (AGO2 Human)
Tags3xFLAGExpressionMammalianMutationSerine 387 to aspartatePromoterCMVAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Sik-WT
Plasmid#210722PurposeConstruct for stable expression of mouse wild type Ptk6 (Sik) in mammalian cells.DepositorInsertProtein tyrosine kinase 6 (Ptk6 Mouse)
ExpressionMammalianAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
EK0445 SFFV-mCherry-SGc(DNAJB1.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191155PurposeExpression of a sensor for human DNAJB1 (hsp40) 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTRDepositorInsertmCherry:DNAJB1.s1:EGFP-3xMS2
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
LB1366 (Bcl-2 promoter with MHS1234)
Plasmid#15388DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 full promoter with IgH full enhancer MHS1234Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-GABARAPL2 G116
Plasmid#123105PurposeExpresses 3xFLAG-GABARAPL2 G116 in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
Tags3xFLAGExpressionMammalianMutationDeleted amino acid 117. Stop codon after G116PromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV Gab1(PRD)-mCherry-HOTag3
Plasmid#190026PurposeTransiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.DepositorInsertGab1(PRD) CluMPS
ExpressionMammalianPromoterCMVAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVPS13D-I-EGFP
Plasmid#176464Purposeexpress internally GFP-tagged VPS13D in mammalian cellsDepositorInsertVPS13D (VPS13D Human)
TagsGFP-UbExpressionMammalianMutationEYFP is internally insertedPromoterCMVAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMED5-bio-His
Plasmid#53338PurposeExpresses full-length Transmembrane emp24 domain-containing protein 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTMED5 (TMED5 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Arch(D95H)-eGFP
Plasmid#51081PurposeExpresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltageDepositorInsertArchaerhodopsin-3 w/ D95H point mutation fused to eGFP
UseLentiviralTagseGFPExpressionMammalianMutationChanged Aspartic Acid 95 to HistidinePromoterUbiquitinAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
A2M-bio-His
Plasmid#53423PurposeExpresses full-length Alpha-2-macroglobulin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertA2M (A2M Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only