165,343 results

1. MAC_N_STA5A

   Plasmid

   #167799

   Purpose

   Expresses STA5A in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   STA5A (STAT5A Human)
   

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-CAG-hCas3

   Plasmid

   #134920

   Purpose

   Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cas3 with bpNLS
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGT-S1

   Plasmid

   #122582

   Purpose

   MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   beta-lactamase/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBbi-RP

   Plasmid

   #60513

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS SK mCherryROSAegfp

   Plasmid

   #54322

   Purpose

   Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

   Depositor

   Silvestro Conticello

   Article

   Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

   Insert

   mCherry and EGFP
   

   Use

   CRISPR, Mouse Targeting, and TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6xHis-SNAP-PI(3)P Probe

   Plasmid

   #211508

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   FYVE-Hrs x 2 (PIKFYVE Human)
   

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CycK-GFP (1-270AA) in Cilantro (Codon optimized)

   Plasmid

   #169930

   Purpose

   Overexpression of 1-270AA CycK-GFP fusion

   Depositor

   Benjamin Ebert

   Article

   Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

   Insert

   CCNK (CCNK Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Available Since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-p18-HA

   Plasmid

   #42338

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

   Insert

   p18 (LAMTOR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

9. BcatMutS33/S37.T41/S45

   Plasmid

   #24204

   Depositor

   David Rimm

   Insert

   beta catenin (CTNNB1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S33A, S37A, T41A,S45A

   Promoter

   CMV

   Available Since

   March 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. ECFP-TevS-YPET

    Plasmid

    #100097

    Purpose

    FRET-based ECFP-YPET reporter that is cleaved by TEV

    Depositor

    Jim Wells

    Article

    Gray et al Cell. 2010 Aug 20;142(4):637-46. doi: 10

    Insert

    ECFP-TevS-YPET
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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