Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,250 results for: pcdna3.1

Showing: 2321 - 2360 of 5250 results

  5. pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

    Plasmid
    #228400
    Purpose
    Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm
    Depositor
    Dirk Daelemans
    Article
    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:
    Insert
    NES/PKI-mNeonGreen2x-NLS/FUS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    228400-plasmid-map-sequence-id-457259

    Enlarge

  6. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid
    #182682
    Purpose
    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Used as a donor for endogenous NFL tagging via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    NA
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182682-plasmid-map-sequence-id-363082

    Enlarge

  7. pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid
    #182685
    Purpose
    Encodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and NA
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182685-plasmid-map-sequence-id-363019

    Enlarge

  8. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion (NT96)

    Plasmid
    #50914
    Purpose
    Expresses human NKCC1 with truncation of N-terminus and mutations at P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C I730C N-term deletion of aa13-222 in hNKCC1…
    Promoter
    CMV
    Available since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    50914-plasmid-map-sequence-id-76387

    Enlarge

  9. pcDNA3.1 Flag YFP hNKCC1 A675C C723S C724V A735C (NT847)

    Plasmid
    #50865
    Purpose
    Expresses human NKCC1 A675C C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A675C C723S C724V A735C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50865-plasmid-map-sequence-id-76338

    Enlarge

  10. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations P676C I730C (NT95)

    Plasmid
    #50913
    Purpose
    Expresses human NKCC1 with mutated Ser and Thr residues in the N-terminus and mutations at P676C I730C. Contains N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    S150N S155Q S170Q T177A S183R S193E T203A T205A T…
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50913-plasmid-map-sequence-id-76386

    Enlarge

  12. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(TAG)-3xFLAG donor

    Plasmid
    #182686
    Purpose
    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linkerA6TAG-3xFLAG. Can be used as a donor for the tagging of endogenous NFL via TKIT approach and click labeling of NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse Nefl intron3-exon4-linker(TAG)-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    NA
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182686-plasmid-map-sequence-id-363076

    Enlarge

  13. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94)

    Plasmid
    #50912
    Purpose
    Expresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C and I730C; N-term deletion of aa13-222; C-t…
    Promoter
    CMV
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    50912-plasmid-map-sequence-id-76385

    Enlarge

  14. pcmCherry 3.1(-)

    Plasmid
    #62803
    Purpose
    Modified pcDNA3.1(-), with Neomycin resistance cassette replaced by mCherry
    Depositor
    Michal Minczuk
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    62803-plasmid-map-sequence-id-103515

    Enlarge

  15. pUB2.1

    Plasmid
    #40728
    Purpose
    Modified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert background
    Depositor
    Thomas Hughes
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    40728-plasmid-map-sequence-id-128586

    Enlarge

  16. pET3C-ARL16(CO)-His6

    Plasmid
    #216485
    Purpose
    Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.
    Depositor
    Richard Kahn
    Insert
    ADP-ribosylation factor-like 16 (ARL16 Human)
    Use
    Tags
    includes GSGSGSHHHHHH (His6) tag at C-terminus
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216485-plasmid-map-sequence-id-426900

    Enlarge

  17. pEZYmyc-His

    Plasmid
    #18701
    Purpose
    pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells
    Depositor
    Yu-Zhu Zhang
    Article
    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.
    Type
    Empty backbone
    Use
    Tags
    Myc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    18701-plasmid-map-sequence-id-181026

    Enlarge

  19. pOME240P

    Plasmid
    #182602
    Purpose
    T15
    Depositor
    Dirk Dittmer
    Insert
    T15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    182602-plasmid-map-sequence-id-359741

    Enlarge

  31. Nemato

    Plasmid
    #24006
    Depositor
    Lawrence Cohen
    Insert
    VSFP
    Use
    Tags
    Cerulean and Citruline
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 2, 2010
    Availability
    Academic Institutions and Nonprofits only
    24006-plasmid-map-sequence-id-11277

    Enlarge

  32. pOME0294M

    Plasmid
    #182623
    Purpose
    KSHV anti-RTA_3Kb_Reverse
    Depositor
    Dirk Dittmer
    Insert
    KSHV anti-RTA_3Kb_Reverse
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion at 2853
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    182623-plasmid-map-sequence-id-367109

    Enlarge

Showing: 2321 - 2360 of 5250 results