Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,154 results for: dre

Showing: 2901 - 2950 of 5154 results

  7. pTT3-ecdCD19-ST3-His_withSUMO

    Plasmid
    #223219
    Purpose
    Expression of the extracellular domain of human CD19 fused to Spytag003 + Histag in HEK293(or similar) with SUMO for better protein expression
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    extracellular domain of B-lymphocyte antigen CD19 (CD19 Human)
    Use
    Tags
    Histag, SUMO, and Spytag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    223219-plasmid-map-sequence-id-443124

    Enlarge

  10. pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3

    Plasmid
    #73208
    Purpose
    Expresses dL5(E52D)-mCer3 fusion protein in mitochondria of mammalian cells. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    COXIV-COX8-dL5-2XG4S-mCer3 (COX8A Human, Synthetic)
    Use
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73208-plasmid-map-sequence-id-139728

    Enlarge

  15. pEGFP-dnMCAK

    Plasmid
    #208650
    Purpose
    Mammalian expression plasmid expressing eGFP-dnMCAK driven by CMV promoter
    Depositor
    Samuel Bakhoum
    Article
    Li et al Nature. 2023 Aug;620(7976):1080-1088. do
    Insert
    Kif2c (Kif2c Cricetulus griseus (Chinese hamster))
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    S92A , S106A , S108A, S112A , S186A, H530A, R534A…
    Promoter
    Available since
    Dec. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    208650-plasmid-map-sequence-id-416926

    Enlarge

  16. pcDNA3.1-kappa-myc-dL5-2xG4S-TMst

    Plasmid
    #73206
    Purpose
    Expresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    kappa-myc-dL5-2XG4S-TM (MYC Human)
    Use
    Tags
    The FAP is fused with 2 copies of a G4S linker an…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73206-plasmid-map-sequence-id-139721

    Enlarge

  21. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid
    #162877
    Purpose
    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Insert consists of AA1-170
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162877-plasmid-map-sequence-id-321985

    Enlarge

  25. pBactin-PACT-mOrange2-hCM1

    Plasmid
    #196868
    Purpose
    Expression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosome
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    PACT-mOrange2-hCM1 (Akap9 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196868-plasmid-map-sequence-id-391838

    Enlarge

  27. pMSCVpuro-Mito-DsRed1

    Plasmid
    #87379
    Purpose
    Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).
    Depositor
    Björn Stork
    Article
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    TXN2 mitochondrial transit peptide (TXN2 Human)
    Use
    Retroviral
    Tags
    DsRed1
    Expression
    Mammalian
    Mutation
    First 60 aa only
    Promoter
    Retroviral 5' LTR promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87379-plasmid-map-sequence-id-212256

    Enlarge

  28. pLenti-nSpCas9-APOBEC3A

    Plasmid
    #209040
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC3A-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209040-plasmid-map-sequence-id-413592

    Enlarge

  29. pAS_4xhyb PylT A41AA C55A CRFR1 95TAG 263TAA-HA

    Plasmid
    #154781
    Purpose
    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAG 263TAA, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    CRFR1 (Crhr1 Rat)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    95TAG 263TAA in CRFR1-HA, hybrid PylT with A41AA …
    Promoter
    EF1
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154781-plasmid-map-sequence-id-310400

    Enlarge

  30. Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

    Plasmid
    #207355
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Marianne Carlon
    Article
    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d
    Insert
    RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207355-plasmid-map-sequence-id-408416

    Enlarge

  31. pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA

    Plasmid
    #154780
    Purpose
    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAA 263TAG, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    CRFR1 (Crhr1 Rat)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    95TAA 263TAG in CRFR1-HA, hybrid PylT with A41AA …
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154780-plasmid-map-sequence-id-310401

    Enlarge

  34. pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3

    Plasmid
    #73205
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    NLS-myc-dL5-2XG4S-mCer3 (MYC Human, Synthetic)
    Use
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73205-plasmid-map-sequence-id-139718

    Enlarge

  37. pTT3-ecdICAM1-ST3-His

    Plasmid
    #210666
    Purpose
    Expression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    Extracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Human, Synthetic)
    Use
    Tags
    Histag and Spytag003
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    210666-plasmid-map-sequence-id-417657

    Enlarge

  44. pBactin-td-mCherry-Cdk5rap2-CTD

    Plasmid
    #196854
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    td-mCherry-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196854-plasmid-map-sequence-id-391811

    Enlarge

  47. pCW57.1 KBTBD4 R313PRR

    Plasmid
    #184626
    Purpose
    Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 R313PRR expression in mammalian cells
    Depositor
    Vincenzo D'Angiolella
    Article
    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1
    Insert
    N-terminal Flag tagged KBTBD4 R313PRR (KBTBD4 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    R313PRR
    Promoter
    TRE promoter, Tet ON
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184626-plasmid-map-sequence-id-363382

    Enlarge

  48. R620-M13-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.1.7

    Plasmid
    #170197
    Purpose
    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)
    Depositor
    Dominic Esposito
    Article
    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568
    Insert
    SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)
    Use
    Tags
    His6
    Expression
    Mammalian
    Mutation
    Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…
    Promoter
    CMV51
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    170197-plasmid-map-sequence-id-334257

    Enlarge

Showing: 2901 - 2950 of 5154 results