We narrowed to 17,688 results for: SHE

1. pEF_dCas9-VP64

   Plasmid

   #68417

   Purpose

   Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and VP64

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   human EF1[alpha]

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTORKm43GWF

   Plasmid

   #112899

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mWasabi.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

   Type

   Empty backbone

   Use

   Oomycete expression

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFLEX+mCyclin D1

   Plasmid

   #86793

   Purpose

   mammalian expression of cyclin D1

   Depositor

   Charles Sherr, Martine Roussel

   Article

   Matsushime et al Cell. 1991 May 17;65(4):701-13.

   Insert

   cyclin D1 (Ccnd1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. CBP-HAT-GST

   Plasmid

   #21093

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

   Insert

   CBP (Crebbp Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   HAT domain: aa 1196-1718

   Available Since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCGN-S2P-WT

   Plasmid

   #32957

   Purpose

   For mammalian expression of human S2P with an HA tag

   Depositor

   Ron Prywes

   Article

   Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

   Insert

   S2P (MBTPS2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP-C1-CaMKIIbeta(K43R)

   Plasmid

   #21225

   Depositor

   Tobias Meyer

   Article

   Shen et al Science. 1999 Apr 2. 284(5411):162-6.

   Insert

   CaMKIIbeta(K43R) (Camk2b Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CaMKIIbeta(K43R) Lab plasmid CF 0003.

   Available Since

   Sept. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTORKm43GWR

   Plasmid

   #112900

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of tdTomato.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

   Type

   Empty backbone

   Use

   Oomycete expression

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. SHC003BSD-DelGFP

   Plasmid

   #133301

   Purpose

   EGFP reporter was removed and the selection marker was modified as blasticidin in the construct of SHC003. This construct could be used for expression of a gene by lentiviral approach.

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-CaMKIIbeta(A303R)

   Plasmid

   #21224

   Depositor

   Tobias Meyer

   Article

   Shen et al Science. 1999 Apr 2. 284(5411):162-6.

   Insert

   CaMKIIbeta(A303R) (Camk2b Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CaMKIIbeta(A303R) (Lab plasmid CF 0002)

   Available Since

   July 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5 HA3-eDHFR-T2A-neo

    Plasmid

    #86396

    Purpose

    for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

    Depositor

    David Bentley

    Article

    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

    Insert

    HA3 eDHFR T2A neo
    

    Use

    CRISPR

    Tags

    HA3 eDHFR T2A neo

    Mutation

    R12Y, G67S and Y100I mutations in E coli DHFR

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. HBT-GCaMP6-HA

    Plasmid

    #107433

    Purpose

    For Arabidopsis mesophyll protoplast transient expression assay

    Depositor

    Jen Sheen

    Article

    Liu et al Nature. 2017 May 18;545(7654):311-316. d

    Insert

    GCaMP6S
    

    Tags

    HA

    Expression

    Bacterial

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET30a-FluB-NP

    Plasmid

    #165093

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Influenza B virus(B/Georgia/05/2019) nucleocapsid protein
    

    Expression

    Bacterial

    Available Since

    Feb. 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. RVD-AA5

    Plasmid

    #173240

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Insert

    NI-NI dimer RVD at Position5
    

    Use

    Unspecified

    Mutation

    no

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. RVD-AA9

    Plasmid

    #173244

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Insert

    NI-NI dimer RVD at Position9
    

    Use

    Unspecified

    Mutation

    no

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-MTS-ccdb-DddA(G1333C)-UGI-Puro

    Plasmid

    #173228

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-MTS-ccdb-DddA(G1333N)-UGI-Puro

    Plasmid

    #173229

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. dFP-mini

    Plasmid

    #120543

    Purpose

    Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

    Depositor

    Brian Chow

    Article

    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

    Insert

    de novo fluorescence-activating protein mini (dFP-mini)
    

    Tags

    6xHis - TEV cleavage

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. punc-119cR

    Plasmid

    #21596

    Depositor

    Al Fisher

    Article

    Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

    Insert

    unc-119:mCherry (unc-119 Nematode)
    

    Use

    Adds unc-119 marker to amp resistant plasmids

    Tags

    mCherry

    Expression

    Worm

    Mutation

    N/A

    Available Since

    Aug. 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU1/Sm/3' Box_(GLuc)_TOP1

    Plasmid

    #68437

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U1

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. SHC003BSD

    Plasmid

    #133300

    Purpose

    The puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.

    Depositor

    Jingshi Shen

    Article

    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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