We narrowed to 2,983 results for: erf

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pHage2-EF1a-mCherryTRF1-IRES-Blast

Plasmid

#182588

Purpose

expresses mcherry-trf1 in mammalian cells

Depositor

Agnel Sfeir

Publication

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hTRF1 (TERF1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

EF-1a

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 PDGFRB

Plasmid

#67130

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Publication

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PDGFRB (PDGFRB Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-p19-T7-Vif

Plasmid

#46313

Depositor

Judy Lieberman

Publication

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
Vif DNA-1
linker
Vif DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSpyB-GFP

Plasmid

#220190

Purpose

sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG35

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyC-GFP

Plasmid

#220191

Purpose

sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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DT034A

Plasmid

#159395

Purpose

Constitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter

Depositor

Sheref Mansy

Publication

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt

Use

Synthetic Biology

Tags

FLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…

Expression

Bacterial

Promoter

J23117 and pLux promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-SF3B1-K700E-reporter

Plasmid

#200318

Purpose

A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

Depositor

Lili Wang

Publication

Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

Insert

BSD-2A-EGFPi

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-a

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCytERM-mhYFP

Plasmid

#186530

Purpose

Visualization of the ER in cells using monomeric hyperfolder YFP (compatible with OSER assay)

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Monomeric hyperfolder YFP

Tags

Cytochrome p450 first 29 animo acids (Oryctolagus…

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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EC2_10_dCas9_VPR_HC_sgRNA

Plasmid

#163712

Purpose

Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

Depositor

Yvonne Nygård

Publication

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VPR

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_4_dCas9_VPR_sgRNA

Plasmid

#163709

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

Depositor

Yvonne Nygård

Publication

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VPR

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-p19-T7-PLK1

Plasmid

#46312

Depositor

Judy Lieberman

Publication

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
PLK1 DNA-1
linker
PLK1 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pWZL Hygro-NMYC TRF2 deltaB

Plasmid

#16349

Depositor

Titia de Lange

Publication

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Deleted Basic Domain (aa1-44)

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pWZL Hygro-TRF2 deltaB

Plasmid

#18010

Depositor

Titia de Lange

Publication

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Deleted Basic Domain (aa1-44)

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pCMV3Tag8li-hTXLNA-HA

Plasmid

#165472

Purpose

Human TXLNA mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Publication

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

taxilin alpha (TXLNA Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-N1-PDZ1-GAGA

Plasmid

#31651

Depositor

Edward Weinman

Publication

Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Tags

His, S-tag

Expression

Mammalian

Mutation

changed P(21)NGYGF changed to H(21)NGAGA

Promoter

cmv

Available since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-N1-PDZ2-GAGA

Plasmid

#32857

Depositor

Edward Weinman

Publication

Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Tags

His,S-tag

Expression

Mammalian

Mutation

PDZ2 P(161)NGYGF mutated to H(161)NGAGA

Promoter

cmv

Available since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-αIFNα-ib

Plasmid

#110798

Purpose

Expression of an intrabody derived from the monoclonal antibody 4EA1 against all mouse interferon α subtypes in mammalian cells

Depositor

Thomas Böldicke

Publication

Bussow et al PLoS One. 2019 Apr 16;14(4):e0215062. do

Insert

intrabody against mouse interferon alpha

Tags

ER retention signal (SEKDEL), ER signal peptide, …

Expression

Mammalian

Promoter

CMV

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-Myc-TRFcM

Plasmid

#44575

Depositor

Eros Lazzerini Denchi

Publication

Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

Insert

chimeric TRF (TERF2, TERF1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

TRF2 DNA-binding domain (aa 444-500) is replaced …

Available since

May 17, 2013

Availability

Academic Institutions and Nonprofits only
pTH371-SUP45-N58A

Plasmid

#29378

Depositor

Tobias von der Haar

Publication

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

N58A mutant gene including genomic sequence 1432 …

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pTH397-SUP45-I222S

Plasmid

#29388

Depositor

Tobias von der Haar

Publication

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

I222S mutant gene (corresponding to the sup45-2 a…

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article