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pGEX Csk-SH2

Plasmid

#46420

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Csk (CSK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 74-177

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pHH0103_BCL2L1_BH4+Bcl-2

Plasmid

#109993

Purpose

Protein expression and purification of BCL2L1_BH4+Bcl-2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

BCL2L1_BH4+Bcl-2 (BCL2L1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

Plasmid

#47455

Purpose

LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

NLS(alpha-imp)-CRY2PHR-NLS-VP64

Use

AAV

Tags

2A_GFP

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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MSCVpuro-6xHis_SUMO2

Plasmid

#164938

Purpose

Expresses 6xHis tagged human Small Ubiquitin Like Modifier 2 (SUMO2) wild type cDNA

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Small Ubiquitin Like Modifier 2 (SUMO2 Human)

Use

Retroviral

Tags

6XHIS

Promoter

PGK promoter

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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GABPA_pet28a

Plasmid

#131640

Purpose

Bacterial expression of ETS gene GABPA

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

GABPA (GABPA Human)

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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mRuby2-Calnexin-N-14

Plasmid

#55891

Purpose

Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Calnexin (CANX Human)

Tags

mRuby2

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mRuby2-CENPB-N-22

Plasmid

#55893

Purpose

Localization: Nucleus/Centromeres, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

CENPB (CENPB Human)

Tags

mRuby2

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pHH0103_ESRRG_Hormone-recep

Plasmid

#109910

Purpose

Protein expression and purification of ESRRG_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESRRG_Hormone-recep (ESRRG Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-KPNA2

Plasmid

#192306

Purpose

Gateway entry vector for an inducible 3xFLAG-KPNA2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNA2 (KPNA2 Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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mOrange2-Golgi-7

Plasmid

#57959

Purpose

Localization: Gal-T - Golgi Complex, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Golgi (B4GALT1 Human)

Tags

mOrange2

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
2xFKBP-smFP(myc)-KDEL

Plasmid

#173021

Purpose

FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

2xFKBP-smFP(myc)-KDEL

Tags

FKBP and smFP(myc) non-fluorescent

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe HA human Bmf

Plasmid

#17239

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (BMF Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pMMDS DEST

Plasmid

#206264

Purpose

DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Type

Empty backbone

Use

Cre-donor, multimate/gateway dest vector (multiba…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX CrkL-SH2

Plasmid

#46419

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Crkl (CRKL Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 9-108

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pET-deSpCas9-VP64-6xHis

Plasmid

#92117

Purpose

Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive eSpCas9-NLS-3xFLAG-VP64

Use

CRISPR

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A, H840A, K848A, K1003A, R1060A

Promoter

T7

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_VCP_AAA

Plasmid

#109964

Purpose

Protein expression and purification of VCP_AAA

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VCP_AAA (VCP Human)

Expression

Bacterial

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX SHP-1(C)-SH2

Plasmid

#46494

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-1(C) (PTPN6 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 105-217

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pHH0103_EEF1A2_GTP-EFTU

Plasmid

#109862

Purpose

Protein expression and purification of EEF1A2_GTP-EFTU

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EEF1A2_GTP-EFTU (EEF1A2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX Crk-SH2

Plasmid

#46418

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Crk (CRK Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-124

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pYPQ143-ZmUbi

Plasmid

#138107

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by Zea mays Ubi promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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