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pSL3936 (pDonor(AAVS1)_Pse)

Plasmid

#200903

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (AAVS1 NGS)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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Fragment 1 Null

Plasmid

#198729

Purpose

Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

gRNA expression cassette in pENTR4

Use

Gateway Cloning

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pFRT-DHX36iso2

Plasmid

#159586

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 2 in mammalian cells

Depositor

Markus Hafner

Article

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K2-2xCSTREP

Plasmid

#136149

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K2

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-161

Plasmid

#118541

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2 xRor2-mCherry

Plasmid

#67162

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

mCherry

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pJW1510

Plasmid

#62364

Purpose

pFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-mVenus-Ubc6 tail anchor

Expression

Yeast

Mutation

L232H mutation compared to standard mVenus

Promoter

Sec63

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-Sec31A

Plasmid

#226582

Purpose

Encodes Sec31A protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Westrate et al J Cell Sci. 2020 Jul 24;133(14). pii: jc

Insert

Sec31A (Protein transport protein) (SEC31A Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC6A3

Plasmid

#132160

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Sijben et al Sci Rep. 2021 Jan 14;11(1):1312. doi: 10

Insert

SLC6A3 (SLC6A3 Human)

Expression

Mammalian

Available Since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pSL0373 (His-MBP-TniQ)

Plasmid

#130638

Purpose

Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on TniQ

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40

Plasmid

#112496

Purpose

Expresses channel rhodopsin in astrocytes

Depositor

Baljit Khakh

Article

Octeau et al Cell Rep. 2019 May 21;27(8):2249-2261.e7

Insert

ChR2(H134R)

Use

AAV

Tags

mCherry

Mutation

H134R

Promoter

GfaABC1D

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5

Plasmid

#52539

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pGS_129

Plasmid

#160652

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

pZ estradiol inducible promoter

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-SNAP

Plasmid

#174879

Purpose

PiggyBac transposon vector for expression of NLS-SNAP

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

NLS-SNAP

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR1A-SynTetOff-GFP

Plasmid

#190230

Purpose

Gateway entry vector, high-level expression of EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Expression

Bacterial

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSBbi-TIR1-tTA/Pur

Plasmid

#171683

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Inserts

tetracycline-controlled transactivator
Puromycin
EF-1a promoter

Expression

Mammalian

Promoter

EF-1a promoter and RPBSA promoter

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF64-2xNSTREP

Plasmid

#136224

Purpose

Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF64

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF64

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF26-2xCSTREP

Plasmid

#136186

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF26

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF26

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF52-2xCSTREP

Plasmid

#136212

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF52

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF52

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

Plasmid

#130976

Purpose

Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

Depositor

Anne Straube

Article

Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

Insert

Hook3-SNAPf (HOOK3 Human)

Tags

8His-ZZ-LTLT and SNAPf

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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