165,786 results

1. pIRESneo-FLAG/HA Ago2 corrected

   Plasmid

   #10822

   Depositor

   Thomas Tuschl

   Article

   Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

   Insert

   Argonaute 2 (AGO2 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   3 silent mutations in orf

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TRE-fDIO-GFP-IRES-tTA

   Plasmid

   #118026

   Purpose

   AAV vector for sparse and bright labeling in a cell-type specific manner

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

   Insert

   TRE-fDIO-GFP-IRES-tTA
   

   Use

   AAV

   Mutation

   See depositor comments below

   Promoter

   TRE

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-REPORT(EF1a)

   Plasmid

   #172326

   Purpose

   LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. M13KO7-ΔM13-ΔgeneIII-ΔgeneVI

   Plasmid

   #80840

   Purpose

   Modified helper phage

   Depositor

   Mark Isalan

   Article

   Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

   Inserts

   M13 gene II
   M13 gene X
   M13 gene V
   M13 gene VII
   M13 gene IX
   M13 gene VIII
   M13 gene I
   M13 gene XI
   M13 gene IV
   

   Expression

   Bacterial

   Available Since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. EC71174

   Plasmid

   #154067

   Purpose

   Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Inserts

   HS Cre recombinase
   Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
   HYGROMYCIN Resistance Cassette
   

   Use

   Cre/Lox and Synthetic Biology; Golden gate

   Expression

   Bacterial and Plant

   Mutation

   The Arabidopsis thaliana U5 small nuclear ribonuc…

   Promoter

   35S promoter, Hordeum vulgare HSP 17 promoter use…

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lifeact-3xmNG::LEU2

   Plasmid

   #227594

   Purpose

   Integration vector for expressing Lifeact-3xmNeonGreen

   Depositor

   Bruce Goode

   Article

   Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

   Insert

   Lifeact-3xmNeonGreen::LEU2
   

   Tags

   3xmNeonGreen

   Expression

   Yeast

   Mutation

   Truncated ABP140 (aa 1-17 only)

   Promoter

   Abp140

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

   Plasmid

   #83946

   Purpose

   Cas9-carrying vector with kanMX marker

   Depositor

   Irina Borodina

   Article

   Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

   Insert

   Cas9
   

   Expression

   Yeast

   Available Since

   Oct. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. RhoG

   Plasmid

   #55694

   Purpose

   expression of mouse RhoG

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   RhoG (Rhog Mouse)
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-2strep-FBH1

   Plasmid

   #236464

   Purpose

   transient overexpression of FBH1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Jeong et al J Cell Biol. 2013 Jan 21;200(2):141-9. d

   Insert

   FBH1 (FBXO18 Human)
   

   Expression

   Mammalian

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV.CBA.eGFP.2A.wtTau

    Plasmid

    #140424

    Purpose

    AAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTau

    Depositor

    Bradley Hyman

    Article

    Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

    Insert

    eGFP-2A-Tau (MAPT Synthetic, Human)
    

    Use

    AAV

    Tags

    eGFP

    Promoter

    CBA

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFC333

    Plasmid

    #87844

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    ble (bleomycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-2E2-HA-mEGFP

    Plasmid

    #129595

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-C-3xFLAG-Zeo

    Plasmid

    #207739

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. qTAG-C-V5-Blast

    Plasmid

    #207719

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLenti6.3/TO/CD9-GFP

    Plasmid

    #104402

    Purpose

    Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

    Depositor

    Jens Gruber

    Article

    Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

    Insert

    CD9 (CD9 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    CMV

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-NSP5SA11

    Plasmid

    #89172

    Purpose

    Express Rotavirus SA11 NSP5 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP5
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. EK0313 SFFV-mCherry-SG(s70587)SG-Cre (FLP-IN)

    Plasmid

    #191149

    Purpose

    Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, Cre output derived from a gift from prof. Liqun Luo

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:Cre
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEntry_HCoV-229E-Nucleocapsid

    Plasmid

    #168967

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-229E-Nucleocapsid (N )
    

    Use

    Gateway-compatible entry vector

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEntry_HCoV-OC43-Nucleocapsid

    Plasmid

    #168947

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-OC43-Nucleocapsid (EYW02_gp8 )
    

    Use

    Gateway-compatible entry vector

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPhlF-BCD20-sfGFP-ssrA P64U23C39T13 v1-1 (462)

    Plasmid

    #70741

    Purpose

    1 color strong reporter used in study

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Insert

    sfGFP-ssrA
    

    Available Since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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