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amilCP chromoprotein

Plasmid

#117847

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP

Use

Synthetic Biology; Escherichia coli

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-mCherry-KASH

Plasmid

#209200

Purpose

Flp-dependent expression of mCherry tagged KASH protein domain

Depositor

Larry Zweifel

Insert

mCherry-KASH

Use

AAV

Tags

KASH localization domain

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Slo1/BKAlpha maxi-K+ channel scFv [L6/60]

Plasmid

#199421

Purpose

Mammalian expression of Slo1/BKAlpha maxi-K+ channel scFv. Derived from hybridoma L6/60.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingSlo1/BKAlpha maxi-K+ channel (Rat) (Kcnma1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Cyto-mTurq2-Apollo-NADP+

Plasmid

#193301

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasm

Depositor

Jonathan Rocheleau

Article

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Insert

G6PD (G6PD Human)

Tags

mTurqoise2

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1-GalT

Plasmid

#87327

Purpose

To express GalT-mCherry in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

Depositor

Lei Lu

Article

Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

Insert

1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_hTRIM32

Plasmid

#69541

Purpose

Expresses human TRIM32 fused to c-terminal EGFP

Depositor

Martin Dorf

Article

Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960.

Insert

tripartite motif containing 32 (TRIM32 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta K85A pcDNA3

Plasmid

#14755

Depositor

Jim Woodgett

Article

Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

Insert

GSK3 beta K85A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

K85A. Kinase dead mutant.

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX6p1-Rbck1

Plasmid

#63135

Purpose

Expression of human RBCK1/HOIL-1L

Depositor

Titia Sixma

Article

Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

Insert

HOIL-1L (RBCK1 Synthetic, Human)

Tags

GST

Expression

Bacterial

Mutation

Synthetic based on human protein sequence

Promoter

tac

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
Lenti-sgCrebbp#2/Cre

Plasmid

#193210

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgCrebbp#2 (Crebbp Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Ahi1

Plasmid

#30494

Depositor

Joseph Gleeson

Article

Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

Insert

Ahi1 (AHI1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-hG9a

Plasmid

#33025

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a (EHMT2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only

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mscv2.2-NLRC4

Plasmid

#60199

Purpose

Mouse NLRC4 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NLRC4

Depositor

Russell Vance

Article

Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

Insert

NLRC4 (Nlrc4 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pOsU3

Plasmid

#170132

Purpose

For sgRNA expression in monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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LeGO-G2

Plasmid

#25917

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pWS172

Plasmid

#90519

Purpose

Cas9 gap repair vector - HIS3

Depositor

Tom Ellis

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-CAMKIIbeta(T287D)

Plasmid

#21223

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CAMKIIbeta (Camk2b Rat)

Tags

GFP

Expression

Mammalian

Mutation

CAMKIIbeta(T287D) Lab plasmid CF 0001.

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
pRK5-HA-FLCN

Plasmid

#72291

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pWS171

Plasmid

#90518

Purpose

Cas9 gap repair vector - LEU2

Depositor

Tom Ellis

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hsf-1-sgRNA-A

Plasmid

#177786

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hsf-1 (hsf-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF

Plasmid

#74924

Purpose

Mammalian expression of human NSF full-length (744 aa)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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