162,408 results

1. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SRSF7

   Plasmid

   #187582

   Purpose

   Express FLAG epitope and APEX2-tagged SRSF7 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   SRSF7 (SRSF7 Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TAC-OSKM

   Plasmid

   #80481

   Purpose

   piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

   Depositor

   Knut Woltjen

   Article

   Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

   Insert

   OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. plenti-UbcP-HA-CRBN-pGK-HYG

   Plasmid

   #107378

   Purpose

   Lentiviral expression of HA-CRBN

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   UbcP

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTXB1-nbOcIgG-Tn5

   Plasmid

   #184285

   Purpose

   Anti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

   Depositor

   Ivan Raimondi, New York Genome Center

   Article

   Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

   Insert

   nbOcIgG-Tn5
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SRC_HUMAN_D0

   Plasmid

   #79700

   Purpose

   This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   SRC (SRC Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pHTN_HaloTag_KIF5B

   Plasmid

   #213404

   Purpose

   Full-length human kinesin-1 with HaloTag at the N-terminus

   Depositor

   Jonas Ries

   Article

   Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

   Insert

   HaloTag-KIF5B (KIF5B Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEmerald-LaminB1-10

   Plasmid

   #54140

   Purpose

   Localization: Nuclear Envelope, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

   Insert

   LaminB1 (LMNB1 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pT3TS-Tol2

   Plasmid

   #31831

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Insert

   Tol2 Transposase
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Lac

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. β3-integrin-YFP

   Plasmid

   #26653

   Depositor

   Jonathan Jones

   Article

   Tsuruta et al FASEB J. 2002 Jun . 16(8):866-8.

   Insert

   β3-integrin (ITGB3 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCALNL-NaChBac

    Plasmid

    #125580

    Purpose

    Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    NaChBac
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAG-mito-dsRED2

    Plasmid

    #173071

    Purpose

    mitochondria targeted dsRED2 expressed off of CAG promoter

    Depositor

    Thomas Schwarz

    Insert

    Mito
    

    Use

    Lentiviral

    Tags

    dsRED

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. shYAP1 # 1

    Plasmid

    #42540

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDest-565

    Plasmid

    #11520

    Purpose

    Gateway destination vector for bacterial expression; His,GST tag

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    6xHis and GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTH102

    Plasmid

    #84460

    Purpose

    pJB38-NWMN29-30 + SarA_P1-CFP-Term

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    SarA_P1-CFP from pTH2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmGFP11C-­Tau

    Plasmid

    #71434

    Purpose

    Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71433

    Depositor

    Henri Huttunen

    Article

    Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

    Insert

    Tau (0N4R) (MAPT Human)
    

    Use

    Tags

    GFP (213-228)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pIRESpuroN3xFLAG-UFSP2

    Plasmid

    #204553

    Purpose

    Expresses 3xFLAG-tagged UFSP2 in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    UFSP2 (UFSP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYUB1062-GFP

    Plasmid

    #117695

    Purpose

    Express recombinant proteins with GFP-His fusion in Mycobacterium smegmatis

    Depositor

    Elizabeth Fullam

    Article

    Radhakrishnan et al RSC Adv. 2018 Sep 27;8(58):33087-33095.

    Type

    Empty backbone

    Use

    Tags

    GFP and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKT2-ACVR1-G328V-IRES-Katushka

    Plasmid

    #135002

    Purpose

    Expresses ACVR1 with a G328V mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Mendez et al Clin Cancer Res. 2020 Aug 1;26(15):4080-

    Insert

    Activin A receptor, type I (ACVR1) G328V (ACVR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.983G>T, p.G328V

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-ZoAl-RTD-3xF-ORF

    Plasmid

    #238486

    Purpose

    For production of C-term flag-tagged ZoAl RT-dead ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    DD644/645->AA

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-ZoAl-END-3xF-ORF

    Plasmid

    #238485

    Purpose

    For production of C-term flag-tagged ZoAl EN-dead ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    DD1041/1054->AA

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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