164,853 results

1. pAcCas12nHS_RV6_PT1_empty

   Plasmid

   #203810

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6_PT1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pM-Cas12a

   Plasmid

   #196291

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
   

   Use

   CRISPR

   Tags

   Flag

   Expression

   Plant

   Mutation

   See depositor comments below

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-Cre.zf1

   Plasmid

   #61391

   Purpose

   mRNA synthesis of Cre recombinase codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   Cre.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDonor-GDH

   Plasmid

   #140629

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   Mini-Tn6677, T7lac-GDH gene cassette
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPAmCherry-a-tubulin

   Plasmid

   #31930

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

   Insert

   PAmCherry1
   

   Use

   Tags

   a-tubulin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR' CMV GFP

   Plasmid

   #14858

   Depositor

   Inder Verma

   Article

   Miyoshi et al Proc Natl Acad Sci U S A. 1997 Sep 16. 9

   Insert

   CMV-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 11, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. sfGFP-pBAD

   Plasmid

   #54519

   Purpose

   Localization: Protein Expression Vector , Excitation: 485, Emission: 507

   Depositor

   Geoffrey Waldo, Michael Davidson

   Article

   Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

   Type

   Empty backbone

   Use

   Tags

   6xHis and SfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pHR_SFFv_4D5-Low-CAR_RHL001

   Plasmid

   #164824

   Purpose

   Lentiviral vector for constitutive expression of AntiHER2 4D5-Low CAR

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Insert

   AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF1217

   Plasmid

   #142757

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PRRX1 (PRRX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNIR-FbALFA

    Plasmid

    #184678

    Purpose

    NIR-Fb to ALFA-tag

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbALFA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX601-GFP

    Plasmid

    #84040

    Purpose

    Staphylococcus aureus (SaCas9) conjugated with GFP

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDuet1_CoiC_CoiSA

    Plasmid

    #208762

    Purpose

    Two coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CoiC
    CoiSA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    single nucleotide insertion (C70) at RBS of CoiC

    Promoter

    T7

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF0207

    Plasmid

    #141555

    Purpose

    Lentiviral vector for overexpressing the FOXP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXP2 (FOXP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-YTHDC1-FL-mCherry-Cry2

    Plasmid

    #177124

    Purpose

    Express Full-length YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Use

    Tags

    mCherry-CRY2

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTight-hASCL1-N174

    Plasmid

    #31876

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    achaete-scute complex homolog 1 (ASCL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSH87

    Plasmid

    #127705

    Purpose

    Pflp-13::gfp1-10

    Depositor

    David M. Miller

    Article

    He et al Genetics. 2019 Apr 5. pii: genetics.119.

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    flp-13

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.3-NL63-D14

    Plasmid

    #172666

    Purpose

    encodes NL63 spike protein, for pseudovirus production

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    HCNV63gp2 (HCNV63gp2 human coronavirus NL63)
    

    Use

    Lentiviral and Retroviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    c-terminal deletion of 14aa

    Promoter

    CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro

    Plasmid

    #141391

    Purpose

    Lentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-N (N SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pTnsABC

    Plasmid

    #160031

    Purpose

    Expresses V. cholerae TnsABC

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTnsA, VchTnsB, VchTnsC
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    no

    Promoter

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA HA HP1 gamma

    Plasmid

    #24080

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 gamma (CBX3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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