We narrowed to 15,509 results for: LIS
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Plasmid#188399PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptideExpressionBacterialPromoterD-mannoseAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 Tev-Halo tag
Plasmid#117124PurposeC-terminal Tev Halo-TagDepositorInsertTev-Halo-tag Tag
UseGolden gate donor vector for gene targeting in ba…TagsTev-Halo-TagAvailable SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCG-A-sacB(SP)-B
Plasmid#87370PurposeAn ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assemblyDepositorInsertSacB signal peptide
UseSynthetic BiologyAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRY2-INPP5E
Plasmid#79562Purposeexpression of CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motifDepositorInsertINPP5E (INPP5E Human)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationC-terminal region, aa 214_644, C641A mutation to …PromoterCMVAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pLUEF1 Luc2-IRES-GFP
Plasmid#224467PurposeEf1a downstream of a ubiquitous chromatin opening element drives expression of Luciferase2 and IRES GFPDepositorInsertLuciferase
UseLentiviralPromoterEF1aAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-BlastR [M1G]
Plasmid#171814PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]DepositorInsertmScarlet-T2A-BlastR
UseGene taggingMutationBlastR: Changed Methionin 1 to Glycine.Available SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta11 Aphrodite
Plasmid#13569DepositorInsertFLIPglu F16A Delta11 Aphrodite
TagsAphrodite and ECFPExpressionBacterialMutationFluorophore-derived linker shortened. F16A mutati…Available SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAGH10 - RlucFluc minus 1 HIV-1
Plasmid#198224PurposeFluorescence reporters for -1 ribosomal frameshifting in mammalian cellsDepositorInsertsRenilla luciferase
Firefly luciferase
ExpressionMammalianPromoterSV40Available SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPgiCas13b
Plasmid#184833PurposeInducible expression of PgiCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Porphyromonas gingivalis
UseCRISPRExpressionBacterialPromoterT7 promoterAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
PliaI
Plasmid#55177PurposeBacitracin-inducible promoterDepositorInsertliaI promoter
UseSynthetic Biology; Bacillus biobrick boxPromoterliaIAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
EF2451_pET21a(+)
Plasmid#13840DepositorInsertPPAT
TagsHisExpressionBacterialAvailable SinceJune 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.3_M1-gateway-HA
Plasmid#128439Purposelentiviral destination vector with C-terminal HA tagDepositorInsertC-terminal HA tag
UseLentiviralAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
p221- purVSG117UTR
Plasmid#59732PurposeInserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.DepositorInsertVSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
UseFor homologous recombination into vsg221 expressi…Available SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLew90β-GW/T7/PARP SAS/luciferase/Aldolase 3′UTR
Plasmid#48157PurposeTransgenically expresses firefly luciferase protein in T. cruziDepositorInsertluciferase
UseLuciferase; T. cruzi expressionPromoterT7Available SinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMXS-IRES-Blast SHMT2 CD
Plasmid#106302PurposeExpresses SHMT2 K280A catalytic site mutantDepositorInsertserine hydroxymethyltransferase 2 (SHMT2 Human)
UseRetroviralExpressionMammalianMutationK280A catalytic site mutation, Silent mutations d…Available SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_27_pKD46.10
Plasmid#178922PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-ER-mRFP
Plasmid#231180PurposeExpresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, from a lentiviral vectorDepositorInsertER-mRFP
UseLentiviralPromoterCMVAvailable SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only