We narrowed to 31,288 results for: son
-
Plasmid#216289PurposeExpresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher δ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-ε-Lock
Plasmid#216290PurposeExpresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ε-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p4A8_S7T_BC
Plasmid#217832Purpose4A8 variant in barcoded, yeast surface display plasmidDepositorInsert4A8 variant Fab
ExpressionYeastMutationVL: S7TAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
NTSR1-DuET
Plasmid#213356PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSTR4-DuET
Plasmid#213379PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HTR7-DuET
Plasmid#213327PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1177
Plasmid#217961PurposeDual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.DepositorAvailable SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBD_R44Q-IFP2.0C
Plasmid#215749PurposeExpresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensorsDepositorInsertIFP2.0C
ExpressionMammalianPromoterCMVAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES_iSeroSnFR_cytosolic
Plasmid#214132PurposeFluorescent biosensor for intracellular serotonin (5-HT)DepositorInsertiSeroSnFR_cytosolic
TagsMycExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIG-747_CD19-28z_CAR_TFAP4
Plasmid#207489PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertTFAP4, CD19-28z_CAR (TFAP4 Human)
UseCRISPRAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT448
Plasmid#139388PurposeBile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1),
UseSynthetic BiologyPromoterBile Acid inducible PromoterAvailable SinceJan. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT492
Plasmid#139397PurposeBile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5)
UseSynthetic BiologyPromoterBile Acid inducible PromoterAvailable SinceJan. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-TA-ERP2-hcGAS(wt)
Plasmid#208396PurposeTo generate stable cell line expressing human cGAS(wt) using PiggyBac transposon systemDepositorAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRegev_ExonSkip_LandingPad
Plasmid#204186PurposeBackbone for cloning individual exon sequences into beta globin minigene splicing reporter.DepositorInsertBeta globin splicing minigene
UseSynthetic BiologyExpressionMammalianPromoterTruncated CAGAvailable SinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
hKCNQ5(WT).ires.mScarlet-pcDNA3
Plasmid#204361PurposeHeterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)DepositorAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-LbCas12a-Plus-mCherry
Plasmid#191230PurposeTo express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificityDepositorInsertLbCas12a-Plus-P2A-mCherry
UseCRISPRMutationD156R, R883K, R887AAvailable SinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCgVchCAST-crtYf
Plasmid#203816PurposeExpression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)
UseCRISPRExpressionBacterialAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAH-ER-bapaUGAc
Plasmid#201674Purposeboronic acid- and peptide-assisted UDP-GlcNAc sensor in ERDepositorInsertER-bapaUGAc
TagsER leading sequence and ER retention sequenceExpressionMammalianPromoterCMV SP6Available SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG4
Plasmid#193804PurposeProtein production of ChemoG4 in bacteriaDepositorInsertChemoG4
Tags10xHisExpressionBacterialMutationEGFP:A206K; HT7: E143R, E147R, L271EPromoterT7Available SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoG1
Plasmid#193806PurposeCMV overexpression of ChemoG1 in mammalian cell linesDepositorInsertChemoG1
Tags10xHisExpressionMammalianMutationEGFP: A206KPromoterCMVAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only