-
Plasmid#149574Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only
-
HA Raptor mutant 2
Plasmid#8471DepositorInsertRaptor (RPTOR Human)
UseTagshaExpressionMammalianMutation261DLF263-AAA (D261A, L262A, F263A)PromoterAvailable sinceMay 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pEx_Hsp70::attBmut
Plasmid#48878PurposePhiC31 exchange vector with the Hsp70 promoter driving tagRFPt from the landing site. Template for enhancer tests.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
HA Raptor mutant 3
Plasmid#8473DepositorInsertRaptor (RPTOR Human)
UseTagshaExpressionMammalianMutation313NWIF316-AAAA (N313A, W314A, I315A, F316A)PromoterAvailable sinceMay 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P MUTYH_2
Plasmid#160786PurposeSuppress MUTYHDepositorInsertshMUTYH_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P MUTYH_1
Plasmid#160785PurposeSuppress MUTYHDepositorInsertshMUTYH_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMCGL-T7Mutant
Plasmid#32245DepositorInsertGlucosamine-6-phosphate activated ribozyme
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
circRAJ31-Toehold_mutant
Plasmid#69930PurposeExpresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.DepositorInsertplTet-circRAJ31_toeholdmutant
UseSynthetic BiologyTagsExpressionMutation4 bases mutated in toehold of riboregulatorPromoterPltetO1-2Available sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
BcatMut-Tyr604
Plasmid#24196DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsExpressionMammalianMutationY604FPromoterAvailable sinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
BcatMut-Tyr670
Plasmid#24198DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsExpressionMammalianMutationY670FPromoterAvailable sinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
BcatMut-Tyr432
Plasmid#24200DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsExpressionMammalianMutationY432FPromoterAvailable sinceMarch 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK 4x mut G
Plasmid#11320DepositorInsert4 bulged binding sites for CXCR4 siRNA antisense
UseLuciferaseTagsRr-lucExpressionMutationMutation G (see paper) on inner 2 binding sites.PromoterAvailable sinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX-XIAPmut
Plasmid#8341DepositorInsertXIAPmut (XIAP Human)
UseTagsGSTExpressionBacterialMutationH467APromoterAvailable sinceNov. 15, 2005AvailabilityAcademic Institutions and Nonprofits only -
pgex6p-1mciap1mut
Plasmid#11484DepositorInsertmciap1mut (Birc2 Mouse)
UseTagsGSTExpressionBacterialMutation582 to alaninePromoterAvailable sinceMarch 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
hIRmut-GFP
Plasmid#22397DepositorInsertIRmut
UseTagsGFPExpressionMammalianMutationchanged Leu 62 to ProlinePromoterAvailable sinceNov. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pETMutS-E41A
Plasmid#13048DepositorInsertMutS
UseTagsExpressionBacterialMutationmutation Glu41 to Ala full-lengthPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Mut
Plasmid#48164Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of Mut
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PIM2mut1
Plasmid#51849Purposepromoter region of PIM2 lacking the forkhead binding site #2DepositorInsertPIM2 (PIM2 Human)
UseLuciferase; Reporter vectorTagsExpressionMutationPromoterAvailable sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 Q mutant
Plasmid#13696DepositorInsertHPV 16 E7 Q mutant (E7 Human)
UseTagsExpressionMammalianMutationaa 30-37 changed from DSSEEEDE to QSSQQQQQ.PromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK 4x mut I
Plasmid#11322DepositorInsert4 bulged binding sites for CXCR4 siRNA antisense
UseLuciferaseTagsRr-lucExpressionMutationMutation I (see paper) on inner 2 binding sites.PromoterAvailable sinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET23MutS-F39A
Plasmid#13044DepositorInsertMutS
UseTagsExpressionBacterialMutationPhe39Ala mutation Full lengthPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV5a-p37SHPmut_FLAG
Plasmid#225499PurposeExpression of FLAG-tagged mutated p37 proteinDepositorInsertp37 (UBXN2B Human)
UseTagsFLAGExpressionMammalianMutationF215A/E218A/QKL220-222AAAPromoterCMV promoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_Mut1-SM
Plasmid#215918PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut1 (BRAF Human)
UseTagsExpressionMammalianMutationBRAFPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_Mut2-SM
Plasmid#215919PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut2 (BRAF Human)
UseTagsExpressionMammalianMutationBRAFPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_Mut1-CM
Plasmid#215922PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertone complete-matched (CM) sequence of siBRAF_ Mut1 (BRAF Human)
UseTagsExpressionMammalianMutationBRAFPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_Mut2-CM
Plasmid#215923PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertone complete-matched (CM) sequence of siBRAF_ Mut2 (BRAF Human)
UseTagsExpressionMammalianMutationBRAFPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I89-pMUTE_GG0-1
Plasmid#219708PurposeLevel 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pMUTE-GG1
UseSynthetic BiologyTagsExpressionMutationWT promoter with added golden gate linkersPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-spacer-H1
Plasmid#218399Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-MUTHY
Plasmid#215132PurposeExpresses mCherry-MUTHY in mammalian cells from a retroviral vector.DepositorInsertMUTHY (MUTYH Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_CRFmut
Plasmid#208656PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cellsDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only