2,226 results

1. pCMV-PV-NLS-GFP

   Plasmid

   #17300

   Depositor

   Anton Bennett

   Article

   Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

   Insert

   PV-GFP-NLS (Pvalb Rat)
   

   Use

   Tags

   Myc and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. iRFP-PH-PLCdelta1

   Plasmid

   #66841

   Purpose

   expression of near-infrared PI(4,5)P2 biosensor

   Depositor

   Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   PH-PLCdelta1
   

   Use

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. beta-arrestin1 mYFP

   Plasmid

   #36916

   Depositor

   Robert Lefkowitz

   Article

   Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

   Insert

   beta-arrestin1 (Arrb1 Rat)
   

   Use

   Tags

   mYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-PV-NES-GFP

   Plasmid

   #17301

   Depositor

   Anton Bennett

   Article

   Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

   Insert

   PV-NES-GFP (Pvalb Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGolt-mCherry / pGolt3-mCherry

   Plasmid

   #73297

   Purpose

   Visualization of Dendritic Golgi Satellite

   Depositor

   Michael Kreutz

   Article

   Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1

   Inserts

   ER export signal of Scap
   ER export signal of Scap
   TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
   

   Use

   Tags

   ER export and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate early promoter

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

   Plasmid

   #104495

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV PHP.eB, AAV Retrograde, and AAV1

   Insert

   jGCaMP7s
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO_EGFP-GFE3

   Plasmid

   #79871

   Purpose

   For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-GFE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.hSyn.iGluSnFR3.v857.GPI

   Plasmid

   #178331

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Has Service

   AAV1

   Insert

   pAAV hSyn iGluSnFR3 v857.GPI
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   GPI Anchor, IgK-chain, and Myc epi tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-C1 F-tractin-EGFP

   Plasmid

   #58473

   Purpose

   Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Bioarchitecture. 2015 Aug 28:1-14.

   Insert

   F-tractin (Itpka Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-NR2A

    Plasmid

    #17924

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    NMDAR2A (Grin2a Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZac2.1-GfaABC1D-lck-jGCaMP8f

    Plasmid

    #176759

    Purpose

    AAV-mediated expression of membrane bound GCaMP8f under the control of GfaABC1D promoter

    Depositor

    Loren Looger

    Has Service

    AAV1 and AAV9

    Insert

    lck-jGCaMP8f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AnkyrinB-GFP/pEGFP-N1

    Plasmid

    #197327

    Purpose

    Plasmid expressing an antigen targeting AnkyrinB

    Depositor

    Vann Bennett

    Insert

    AnkyrinB (Ank2 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-HA-rat Nrxn1alpha

    Plasmid

    #58266

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 1alpha

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn1alpha (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    see comments

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMK287 (mAID-Hygro)

    Plasmid

    #72825

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Hygro
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBiFC-bFosVC155

    Plasmid

    #22013

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    bFosVC155
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Oct. 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCI-EGFP-NR1 wt

    Plasmid

    #45446

    Depositor

    Robert Malinow, Andres Barria

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR1 (Grin1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pORANGE Tubb3-GFP KI

    Plasmid

    #131497

    Purpose

    Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Tubb3, Tubb3 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTol2-HuC(elavl3)-CaMPARI2

    Plasmid

    #137185

    Purpose

    pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Insert

    HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
    

    Use

    Tol2 plasmid for zebrafish

    Tags

    FLAG, HA, myc

    Expression

    Mutation

    Promoter

    HuC(elavl3)

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-HA-rat Nrxn3beta AS4(-)

    Plasmid

    #58269

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn3beta AS4(-) (Nrxn3 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. DAGR

    Plasmid

    #14865

    Depositor

    Alexandra Newton

    Article

    Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

    Insert

    DAG reporter (Prkcb Rat)
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV5 alpha 1A AR

    Plasmid

    #45760

    Depositor

    Robert Lefkowitz

    Article

    Schwinn et al J Biol Chem. 1990 May 15;265(14):8183-9.

    Insert

    alpha 1A AR (Adra1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

22. PSD-95-pTagRFP

    Plasmid

    #52671

    Purpose

    expression of TagRFP-tagged PSD-95

    Depositor

    Johannes Hell

    Insert

    PSD-95 (Dlg4 Rat)
    

    Use

    Tags

    tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. rGluA2 (px330:2-guideCas9)

    Plasmid

    #169439

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEF Synaptophysin-mRuby

    Plasmid

    #188980

    Purpose

    Synaptic buton marker

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Synaptophysin (Syp Synthetic, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SNAP-OMP25

    Plasmid

    #141149

    Purpose

    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Budding Yeast, Rat)
    

    Use

    Tags

    SNAP tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCI-EGFP-NR2a wt

    Plasmid

    #45445

    Depositor

    Robert Malinow, Andres Barria

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR2A (Grin2a Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-BE1

    Plasmid

    #73019

    Purpose

    Expresses BE1 with C-terminal NLS in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-BE2

    Plasmid

    #73020

    Purpose

    Expresses BE2 in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLAMP1-miRFP670nano3

    Plasmid

    #184669

    Purpose

    LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    LAMP1-miRFP670nano3
    

    Use

    Tags

    miRFP670nano3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CMV-tdTomato-P2A-paCaMKII

    Plasmid

    #165431

    Purpose

    Expresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    tdTomato-P2A-paCaMKII
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge