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We narrowed to 314 results for: GCN4

Showing: 201 - 240 of 314 results

  3. pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid
    #119945
    Purpose
    Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    March 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    119945-plasmid-map-sequence-id-237587

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  5. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174645
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11
    Use
    Tags
    SSPB(micro)-Venus and iLID-mCherry
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174645-plasmid-map-sequence-id-347047

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  6. pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

    Plasmid
    #105000
    Purpose
    Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105000-plasmid-map-sequence-id-203163

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  8. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid
    #119946
    Purpose
    Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    119946-plasmid-map-sequence-id-237566

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  9. pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174647
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    CMV (with TetOn)
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174647-plasmid-map-sequence-id-345296

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  12. pGCNK58

    Plasmid
    #202461
    Purpose
    Plasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cells
    Depositor
    Arthur Palmer, Stephen Harrison
    Article
    Weiss et al Nature. 1990 Oct 11;347(6293):575-8. doi
    Insert
    Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    202461-plasmid-map-sequence-id-403118

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  17. pZF-BCB-GCN

    Plasmid
    #127476
    Purpose
    coding sequence for synthetic zinc finger ZF-BCB-GCN
    Depositor
    Sebastian Maerkl
    Article
    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11
    Insert
    ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
    Use
    Cell-free transcription–translation
    Tags
    GCN4 leucine zipper
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    127476-plasmid-map-sequence-id-248524

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  18. pZF-ADD-GCN

    Plasmid
    #127475
    Purpose
    coding sequence for synthetic zinc finger ZF-ADD-GCN
    Depositor
    Sebastian Maerkl
    Article
    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11
    Insert
    ZF-ADD synthetic transcription factor fused with GCN4 leucine zipper
    Use
    Cell-free transcription–translation
    Tags
    GCN4 leucine zipper
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    127475-plasmid-map-sequence-id-248906

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  22. pM162

    Plasmid
    #173221
    Purpose
    Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1
    Depositor
    Hajin Kim
    Article
    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do
    Inserts
    ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
    MCP-mCherry-GFP11-GB1
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    173221-plasmid-map-sequence-id-341549

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  26. pTH825-CEN-minRLuc/slowstaCFLuc

    Plasmid
    #115370
    Purpose
    Expresses translation elongation-controlled Renilla luciferase and translation initiation-controlled firefly luciferase
    Depositor
    Tobias von der Haar
    Inserts
    Gcn4 5'-UTR + Firefly Luciferase
    Renilla luciferase (codon minimised)
    Use
    Tags
    Expression
    Yeast
    Mutation
    All codons have been changed for the least favour…
    Promoter
    ADH1 and TDH3
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    115370-plasmid-map-sequence-id-238823

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  28. pTH862-CEN-slowmaxRLuc/minCFLuc

    Plasmid
    #115371
    Purpose
    Expresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferase
    Depositor
    Tobias von der Haar
    Inserts
    Firefly Luciferase (codon minimised)
    Gcn4 5'-UTR + Renilla luciferase (codon optimised)
    Use
    Tags
    Expression
    Yeast
    Mutation
    All codons have been changed for the most favoura…
    Promoter
    ADH1 and TDH3
    Available since
    June 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    115371-plasmid-map-sequence-id-247306

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  31. pSX2720

    Plasmid
    #200764
    Purpose
    Expresses Superfolder GFP-tag fused single change variable fragment antibody of GCN4 (Suntag) with the tissue-specific promoter col-19 in C. elegans.
    Depositor
    Suhong Xu
    Article
    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755
    Insert
    scFv-sfGFP
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    col-19
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    200764-plasmid-map-sequence-id-400888

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  36. pUbC-OsTIR1-myc-IRES-scFv-sfGFP

    Plasmid
    #84563
    Purpose
    Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP
    Depositor
    Robert Singer
    Article
    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d
    Insert
    Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
    Use
    Lentiviral
    Tags
    myc tag (9x)
    Expression
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    84563-plasmid-map-sequence-id-176790

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  38. phage UbiC scAB-GFP

    Plasmid
    #104998
    Purpose
    Lentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling
    Depositor
    Jeffrey Chao
    Article
    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.
    Insert
    single chain Antibody against GCN4
    Use
    Lentiviral
    Tags
    GB1, HA tag, and sfGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    UbiC
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    104998-plasmid-map-sequence-id-202897

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  40. p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

    Plasmid
    #52895
    Purpose
    Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Zipp.-L-IFP-F[1]
    Use
    Tags
    L-IFP-F[1]
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 201 - 240 of 314 results