We narrowed to 11,550 results for: SHA

1. pAAV-hsyn_NES-His-rsCaMPARI-mRuby3

   Plasmid

   #120805

   Purpose

   AAV plasmid for expressing rsCaMPARI in neurons, nucleus excluded

   Depositor

   Eric Schreiter

   Article

   Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

   Insert

   rsCaMPARI
   

   Use

   AAV

   Tags

   NES-His and mRuby3

   Expression

   Mutation

   Promoter

   hsyn (synapsin-1)

   Available since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTol2-elavl3_NES-rsCaMPARI-mRuby3

   Plasmid

   #122129

   Purpose

   Pan-neuronal expression of rsCaMPARI in zebrafish, nucleus excluded

   Depositor

   Eric Schreiter

   Article

   Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

   Insert

   rsCaMPARI
   

   Use

   Zebrafish expression

   Tags

   NES-His and mRuby3

   Expression

   Mutation

   Promoter

   elavl3

   Available since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hsyn_NLS-His-rsCaMPARI-mRuby3

   Plasmid

   #122092

   Purpose

   AAV plasmid for expressing rsCaMPARI in neurons, nucleus localized

   Depositor

   Eric Schreiter

   Article

   Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

   Insert

   rsCaMPARI
   

   Use

   AAV

   Tags

   NLS-His and mRuby3

   Expression

   Mutation

   Promoter

   hsyn (synapsin-1)

   Available since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLC25A13-APEX2

   Plasmid

   #228198

   Purpose

   plasmid encoding human SLC25A13 with an APEX protein tag

   Depositor

   Stephen Benkovic

   Article

   Sha et al J Biol Chem. 2024 Aug 2;300(9):107620. d

   Insert

   SLC25A13 (SLC25A13 Human)
   

   Use

   Synthetic Biology

   Tags

   APEX2

   Expression

   Mutation

   Promoter

   M13

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CMV-HA-hTAF4b

   Plasmid

   #17998

   Depositor

   Robert Tjian

   Article

   Freiman et al Science. 2001 Sep 14. 293(5537):2084-7.

   Insert

   hTAF4b (TAF4B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa1-64 deleted

   Promoter

   Available since

   May 28, 2008

   Availability

   Academic Institutions and Nonprofits only

6. CFP-NS1

   Plasmid

   #85739

   Purpose

   expresses CFP-FLAG-tagged NS1 monobody.

   Depositor

   John O'Bryan

   Article

   Spencer-Smith et al Nat Chem Biol. 2016 Nov 7. doi: 10.1038/

   Insert

   FLAG-tagged NS1 monobody
   

   Use

   Tags

   ECFP and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGGG_Sr1644-1Sh

   Plasmid

   #164087

   Purpose

   Expresses pGGG_Sr1644-1Sh in plants

   Depositor

   Brande Wulff

   Article

   Yu et al Nat Commun. 2022 Mar 25;13(1):1607. doi:

   Insert

   pGGG_Sr-1644-1Sh
   

   Use

   Tags

   none

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Native promoter

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSC38

   Plasmid

   #104812

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr3g107390 (MtRdr6). Also expresses Cas9 from AtUBQ10 promoter.

   Depositor

   Nevin Young

   Insert

   Medtr3g107390
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSC49

   Plasmid

   #104823

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Glyma17g11235 (Dcl4a). Also expresses Cas9 from AtUBQ10 promoter.

   Depositor

   Robert Stupar

   Insert

   Glyma17g11235
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSC37

    Plasmid

    #104811

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr3g107390 (MtRdr6). Also expresses Cas9 from Gmubi promoter

    Depositor

    Nevin Young

    Insert

    Medtr3g107390
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBABE myc-Raptor

    Plasmid

    #18116

    Depositor

    Reuben Shaw

    Article

    Gwinn et al Mol Cell. 2008 Apr 25. 30(2):214-26.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

12. myc-Raptor S722A/S792A

    Plasmid

    #18118

    Depositor

    Reuben Shaw

    Article

    Gwinn et al Mol Cell. 2008 Apr 25. 30(2):214-26.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S722A, S792A

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pGEX-5X2-RalBP1

    Plasmid

    #37245

    Depositor

    David Kashatus, Christopher Counter

    Article

    Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

    Insert

    RalBP1 (RALBP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pRS712

    Plasmid

    #213041

    Purpose

    CRISPR activation based overexpression in Nakaseomyces (Candida) glabrata

    Depositor

    Rebecca Shapiro

    Article

    Maroc et al mSphere. 2024 Feb 28;9(2):e0076123. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBABE myc-Raptor S722A/S792A

    Plasmid

    #18117

    Depositor

    Reuben Shaw

    Article

    Gwinn et al Mol Cell. 2008 Apr 25. 30(2):214-26.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S722A, S792A

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pRS159

    Plasmid

    #122378

    Purpose

    CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAS1-Prig-3::tagBFP

    Plasmid

    #165598

    Purpose

    rig-3 promoter derive BFP expression in AVA head neurons.

    Depositor

    Andrew Leifer

    Article

    Hallinen et al Elife. 2021 Jul 29;10:e66135. doi: 10.75

    Insert

    Prig-3, tagBFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS143

    Plasmid

    #122377

    Purpose

    CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLC-284

    Plasmid

    #124294

    Purpose

    A low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.

    Depositor

    Swaine Chen

    Article

    Eshaghi et al Pathogens. 2016 Jan 5;5(1). pii: pathoge

    Insert

    vsfGFP-9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS263

    Plasmid

    #122379

    Purpose

    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Insert

    HSP90
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRS267

    Plasmid

    #122380

    Purpose

    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Insert

    HSP90
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pQP-2473

    Plasmid

    #138976

    Purpose

    Nucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pQP-CiRISB

    Plasmid

    #138978

    Purpose

    Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pQP-AR10

    Plasmid

    #138980

    Purpose

    Endogenous RAS regulation using light-controlled intrabody

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    BglII-BphP1-NES-T2A- iB(RAS)-NES-mCherry-QPAS1- NLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pQP-NiRISB

    Plasmid

    #138977

    Purpose

    Light-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pQP-iRISB

    Plasmid

    #138979

    Purpose

    Light-controlled tri-directional targeting of endogenous proteins

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pQP-2363

    Plasmid

    #138975

    Purpose

    Relocalization of genomically expressed protein of interest from cytoplasm to nucleus upon near-infrared illumination

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1- iB(GFP) -IRES2-NES-mCherry-QPAS1- NLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-V5-DGCR8

    Plasmid

    #51383

    Depositor

    Narry Kim

    Article

    Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

    Insert

    DGCR8 (DGCR8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRS156

    Plasmid

    #182707

    Purpose

    CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

    Depositor

    Rebecca Shapiro

    Article

    Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLKO human ULK1 shRNA 8

    Plasmid

    #27633

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    human ULK1 shRNA 8
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLKO human ULK2 shRNA 91

    Plasmid

    #27634

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    human ULK2 shRNA 91
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pLKO mouse ULK2 shRNA 93

    Plasmid

    #27635

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse ULK2 shRNA 93
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

33. pQP-2460

    Plasmid

    #138973

    Purpose

    Light-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRSETC-PITPbeta (splice variant 1)

    Plasmid

    #58270

    Purpose

    bacterial expression of PITP beta (phosphatidylinositol transfer protein beta)

    Depositor

    Shamshad Cockcroft

    Article

    Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

    Insert

    PITP beta (PITPNB Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

35. NmMetNI-WT

    Plasmid

    #172114

    Purpose

    Expression of MetN and MetI membrane proteins

    Depositor

    Douglas Rees

    Article

    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

    Inserts

    MetN
    MetI
    

    Use

    Tags

    10x His Tag and Enterokinase

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pQP-2376

    Plasmid

    #138974

    Purpose

    Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRSETC-PITPbeta (splice variant 1) K61A

    Plasmid

    #58271

    Purpose

    bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

    Depositor

    Shamshad Cockcroft

    Article

    Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

    Insert

    PITP beta (PITPNB Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pEBG‐AMPK
α1(1‐312)

    Plasmid

    #27632

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    AMPK
α1(1‐312) (Prkaa1 Rat)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Constitutively
 active
 by 
truncation 
after 
am…

    Promoter

    Available since

    May 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLV-mScarlet3-CAAX-IRES-Puro

    Plasmid

    #222639

    Purpose

    Live-cell plasma membrane marker

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mScarlet3-CAAX
    

    Use

    Lentiviral

    Tags

    mScarlet3

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcdna3 flag - ULK1 wt

    Plasmid

    #27636

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse ULK1 (Ulk1 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Initial M from ULK1 removed.

    Promoter

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge