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We narrowed to 5,443 results for: 129

Showing: 251 - 300 of 5443 results

  2. pGAN147

    Plasmid
    #129202
    Purpose
    yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone
    Depositor
    Ahmad Khalil
    Article
    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SUP35
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    129202-plasmid-map-sequence-id-255858

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  5. pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

    Plasmid
    #129538
    Purpose
    modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection
    Depositor
    Arneet Saltzman
    Article
    Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox; Mos1-mediated single copy insertion (mos…
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    129538-plasmid-map-sequence-id-258304

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  6. pAH-CTX1-rhadCas9-native

    Plasmid
    #129392
    Purpose
    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    S. pyogenes dCas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine, Histidine 840 to Alanine
    Promoter
    PrhaBAD
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129392-plasmid-map-sequence-id-266824

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  7. pYPQ292

    Plasmid
    #129672
    Purpose
    Gateway entry clone for CRISPR-Cas12b systems
    Depositor
    Yiping Qi
    Article
    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:
    Insert
    AaCas12b
    Use
    CRISPR; Gateway compatible aacas12b entry clone
    Tags
    Expression
    Plant
    Mutation
    AaCas12b is rice codon optimized
    Promoter
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    129672-plasmid-map-sequence-id-255064

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  10. pAAV-CAG-DIO-(STChRger2-TS-EYFP)

    Plasmid
    #129394
    Purpose
    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    soma targeted ChRger2
    Use
    AAV and Cre/Lox
    Tags
    Kv2.1-TS-EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129394-plasmid-map-sequence-id-259728

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  13. pLBS.CAG/NLS-mScarlet

    Plasmid
    #129336
    Purpose
    nucleus marker, lentivirus
    Depositor
    Yasushi Okada
    Insert
    mScarlet (Addgene 85042)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 28, 2019
    Availability
    Academic Institutions and Nonprofits only

  14. pGEX-4T1 NLS-GFP

    Plasmid
    #129441
    Purpose
    bacterial expression of GFP
    Depositor
    Oskar Laur
    Insert
    GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129441-plasmid-map-sequence-id-256454

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  15. pMM0641

    Plasmid
    #129006
    Purpose
    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129006-plasmid-map-sequence-id-259445

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  19. pN1-OPA1(123Nt)-Halo

    Plasmid
    #129345
    Purpose
    Halo Tag, Mitochondrial intermembrane space localization
    Depositor
    Yasushi Okada
    Insert
    Halo (Promega)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    129345-plasmid-map-sequence-id-260109

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  21. pExp-Etag

    Plasmid
    #129247
    Purpose
    Protein expression in E.coli
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    His and Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    129247-plasmid-map-sequence-id-252355

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  22. pN1-OPA1(123Nt)-mNG

    Plasmid
    #129344
    Purpose
    Mitochondrial intermembrane space localization
    Depositor
    Yasushi Okada
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    129344-plasmid-map-sequence-id-260107

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  24. Lenti puro EF2 LUC-2A-Dendra2

    Plasmid
    #129427
    Purpose
    expression of LUC and Dendra2 reporters under EF2 promoter
    Depositor
    Oskar Laur
    Insert
    LUC Dendra2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129427-plasmid-map-sequence-id-255690

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  28. pCK354

    Plasmid
    #129698
    Purpose
    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799
    Depositor
    John Heap
    Article
    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m
    Inserts
    PrhaBAD
    yfp
    rhaS
    Terminator ECK120010799
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    129698-plasmid-map-sequence-id-258484

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  29. pAH-CTX1-rha

    Plasmid
    #129390
    Purpose
    Derived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Rhamnose-inducible promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N/A
    Promoter
    PrhaBAD
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129390-plasmid-map-sequence-id-266815

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  35. pRS426 TEF mCherry

    Plasmid
    #129433
    Purpose
    express mcherry
    Depositor
    Oskar Laur
    Insert
    mcherry
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129433-plasmid-map-sequence-id-256112

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  36. pN1-COX8-mNG

    Plasmid
    #129342
    Purpose
    Mitochondria matrix localization
    Depositor
    Yasushi Okada
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    129342-plasmid-map-sequence-id-260105

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  38. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129028
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129028-plasmid-map-sequence-id-256131

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  40. pSK267

    Plasmid
    #129272
    Purpose
    "ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein
    Depositor
    Ahmad Khalil
    Article
    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi
    Insert
    NLS-VP16-ZF 43-8-NES
    Use
    Tags
    6xHA
    Expression
    Yeast
    Mutation
    Promoter
    SUP35
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    129272-plasmid-map-sequence-id-255863

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  41. pAAV-hSyn-STChRger2-TS-EYFP

    Plasmid
    #129395
    Purpose
    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    soma targeted ChRger2
    Use
    AAV
    Tags
    Kv2.1-TS-EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129395-plasmid-map-sequence-id-258248

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  45. EF1a CHR2 mCherry WPRE

    Plasmid
    #129432
    Purpose
    expression of CHR2 mCherry from EF1a promoter
    Depositor
    Oskar Laur
    Insert
    CHR2 mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129432-plasmid-map-sequence-id-269136

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  46. pLL1270

    Plasmid
    #129281
    Purpose
    The vector used for enzyme expression in Thermoanaerobacterium. thermosaccharolyticum throughout this study. Contains the CBP promoter from Clostridium thermocellum
    Depositor
    Lee Lynd
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Clostridium thermocellum DSM1313 Cellobiose Phosp…
    Available since
    March 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    129281-plasmid-map-sequence-id-260567

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  48. K560_AviN_coBirA

    Plasmid
    #129766
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129766-plasmid-map-sequence-id-263552

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  49. pNCST-AvicFP4

    Plasmid
    #129507
    Purpose
    Expresses AvicFP4 constitutively in E. coli (most strains)
    Depositor
    Nathan Shaner
    Article
    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d
    Insert
    AvicFP4
    Use
    Tags
    Expression
    Bacterial
    Mutation
    One of two variants of AvicFP4 tested with essent…
    Promoter
    synthetic constitutive (stationary phase) promote…
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    129507-plasmid-map-sequence-id-271859

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Showing: 251 - 300 of 5443 results