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Plasmid#124233PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomatoDepositorInsertCoChR-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM51323
Plasmid#153224PurposeLevel 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminatorDepositorInsertArabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:GtACR1-tdTomato
Plasmid#124235PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR1 fused to the red fluorescent protein tdTomatoDepositorInsertGtACR1-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:Chronos-tdTomato
Plasmid#124232PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin Chronos fused to the red fluorescent protein tdTomatoDepositorInsertChronos-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChrimsonR-LSSmOrange
Plasmid#149691PurposeFor packaging and expression of ChrimsonR-LSSmOrange in neurons via AAVDepositorInsertChrimsonR
UseAAVTagsLSSmOrangeExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLBC0120
Plasmid#172791PurposeLevel 0 module containing CDS2ns_mHSP21DepositorInsertCDS mature HSP21 (44-227), Arabidopsis thaliana
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBC0130
Plasmid#172792PurposeLevel 0 module containing CDS2ns_mPTAC5DepositorInsertCDS mature PTAC5 (41-387), Arabidopsis thaliana
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV EF1a DIO iChloC 2A dsRed
Plasmid#70762PurposeAn improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)DepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVTagsExpressionMammalianMutationE83Q,E90R,E101S,D156N,T159CPromoterEF1aAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Chronos-mT-Sapphire
Plasmid#149690PurposeFor packaging and expression of Chronos-mT-Sapphire in neurons via AAVDepositorInsertChronos
UseAAVTagsmT-SapphireExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-C+F-ArchT-GFP
Plasmid#209700PurposeExpresses ArchT-GFP dependent on the combined activity of CRE- and FLP-recombinasesDepositorInsertArchT-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGE1109
Plasmid#202773PurposeCRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CIBN-CreC
Plasmid#75267PurposeThe first half of the photo-activatable cre recombinaseDepositorInsertN-terminal portion of the CIB1 transcription factor
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLBC0010
Plasmid#172774PurposeLevel 0 module containing NT1_CTPSSUDepositorInsertGene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBC0011
Plasmid#172775PurposeLevel 0 module containing NT2_CTPSSUDepositorInsertGene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGE1177
Plasmid#202775PurposeCRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLBC0131
Plasmid#172793PurposeLevel 0 module containing CDS2ns_deltamPTAC5DepositorInsertCDS mature PTAC5 with C-ter delection (41-252), Arabidopsis thaliana
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Cci-UPO SP
Plasmid#153502PurposeCoprinopsis cinerea Unspecific Peroxygenase Signal Peptide Donor PlasmidDepositorInsertUnspecific peroxygenase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer
Plasmid#66779PurposeThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signalingDepositorInsertshbeRhGC
red fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
raACR-mCerulean3-pAAV2-hSyn
Plasmid#204966PurposePanneuronal expression of the MsACR1 mutant raACR fused to mCerulean3DepositorInsertraACR
UseAAVTagsmCerulean3ExpressionMutationPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHES 946
Plasmid#112644Purposeblue light inducible cryptochrome based optogenetic expression systemDepositorInsertRTG3AD-GSx7-CIB1-SV40NLS (CIB1 Budding Yeast, Mustard Weed, Synthetic)
UseTagsExpressionYeastMutationPromoterADH1Available sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
CapChR2_pmScarlet-N1
Plasmid#188032PurposeExpresses CapChR2-mScarlet in mammalian cellsDepositorInsertCapChR2-mScarlet
UseTagsmScarletExpressionMammalianMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
CapChR2_pUAST-attb
Plasmid#188041PurposeExpresses CapChR2 in DrosophilaDepositorInsertCapChR2
UseTagsnoneExpressionInsectMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterUASAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChrimsonR-LSSmOrange-NRN
Plasmid#149693PurposeFor packaging and expression of ChrimsonR-LSSmOrange featuring a neuritin (NRN) 3' UTR in neurons via AAVDepositorInsertChrimsonR
UseAAVTagsLSSmOrangeExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
SERPINA-bio-His
Plasmid#52182PurposeExpresses full-length Alpha-1-antitrypsin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSERPINA (SERPINA1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:ChR2(H134R)-tdTomato
Plasmid#124237PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomatoDepositorInsertChR2(H134R)-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn ChR E90R-T159C 2A tDimer
Plasmid#52495PurposeHistorical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)DepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVTagsExpressionMammalianMutationE90R, T159CPromoterhuman synapsin-1 and ribosomal skip sequenceAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgE/λ
Plasmid#50365PurposeExpresses grass pollen allergen Phl p 7 specific human IgE/λ antibody isotype (102.1F10-IgE/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human epsilon heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
UseTagsExpressionMammalianMutationPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE
Plasmid#198515PurposeExpresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.DepositorInsertPdCO
UseAAV and Cre/LoxTagsRho1D4 and mScarletExpressionMammalianMutationPromoterEF-1aAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE
Plasmid#198508PurposeExpresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.DepositorInsertPdCO
UseAAVTagsRho1D4 and mScarletExpressionMammalianMutationPromoterCaMKIIα minimal promotor (0.4kb)Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CaMKIIa(0.4)-PdCO-EGFP-WPRE
Plasmid#198514PurposeExpresses optimized PdCO in frame with EGFP under control of minimal CamKIIa promotor.DepositorInsertPdCO
UseAAVTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterCaMKIIα minimal promotor (0.4kb)Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_B1ChR2_TS_mScarlet_ER
Plasmid#195191PurposeHighly K+ selective channelrhodopsinDepositorInsertBilabrum sp. K+ selective channelrhodopsin 2
UseTagsER-export sequence, Kir2.1 trafficking motif, and…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Chronos-mT-Sapphire-NRN
Plasmid#149692PurposeFor packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAVDepositorInsertChronos
UseAAVTagsmT-SapphireExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_WiChR_TS_mScarlet_ER
Plasmid#205997PurposeHigh efficient, K+ selective channelrhodopsin for the inhibition of neuronsDepositorInsertWobblia Inhibitory Channelrhodopsin
UseAAVTagsTS, mScarlet, ERExpressionMutationPromoterhSynAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_WiChR_TS_mScarlet_ER
Plasmid#195190PurposeHigh efficient, K+ selective channelrhodopsin for the inhibition of excitable cellsDepositorInsertWobblia lunata inhibitory Channelrhodopsin
UseTagsER-export sequence, Kir2.1 trafficking motif, and…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-C1C2-EYFP
Plasmid#35519DepositorInsertChR1-ChR2 Chimera
UseLentiviralTagsEYFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2-U6
Plasmid#173157PurposeSingle guide RNA cassette under U6 promoterDepositorInsertsgRNA cassette under U6 promoter
UseTagsExpressionPlantMutationPromoterAtU6-26Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPML1 (GB0045)
Plasmid#68164PurposeProvides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid partDepositorInsertAtML1 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDP085
Plasmid#198425PurposepUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120DepositorInsertE. coli BirA
UseTagsExpressionPlantMutationPromoterpUBQ10Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pHsp18.2 (GB0153)
Plasmid#68171PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHsp70B (GB0155)
Plasmid#68172PurposeProvides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid partDepositorInsertAtHsp70B promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:CheRiff-tdTomato
Plasmid#124234PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CheRiff fused to the red fluorescent protein tdTomatoDepositorInsertCheRiff-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTHsp18.2 (GB0035)
Plasmid#68186PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPAtUbq10 (GB0223)
Plasmid#68174PurposeProvides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid partDepositorInsertAtUbq10 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPAct2noUTR (GB0192)
Plasmid#68177PurposeProvides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid partDepositorInsertAtAct2 promoter (no UTR)
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTAtUbq3 (GB0219)
Plasmid#68192PurposeProvides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid partDepositorInsertAtUbq3 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pTAct2 (GB0210)
Plasmid#68191PurposeProvides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid partDepositorInsertAtAct2 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5'UTRAct2 (GB0193)
Plasmid#68178PurposeProvides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid partDepositorInsert5'UTR AtAct2
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only