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Addgene

We narrowed to 7,012 results for: RAP

Showing: 251 - 300 of 7012 results
  1. pGAPTrap-Clover-Muro

    Plasmid
    #82334
    Purpose
    Vector targeting the Clover gene to the GAPDH locus of human cells. Clover is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Insert
    Clover-T2AMuro
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pGAPTrap-eGFP-IRESMeo

    Plasmid
    #82502
    Purpose
    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. G418 resistance is encoded by the Meo gene.
    Depositor
    Insert
    eGFP-IRESMeo
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. miR-451 Trap

    Plasmid
    #61487
    Purpose
    to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoter
    Depositor
    Insert
    miR-451 Trap
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional phosphoglycerate kinase promoter (b…
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid
    #61966
    Purpose
    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))
    Depositor
    Inserts
    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD and PLtetO
    Available since
    Feb. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pBactin-td-mCherry-Cdk5rap2-CTD

    Plasmid
    #196854
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Insert
    td-mCherry-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pBactin-AcGFP-Cdk5rap2-CTD

    Plasmid
    #196853
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to AcGFP. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Insert
    AcGFP-Cdk5rap2-CTD (Cdk5rap2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. CbSBS-Flag/TRRAP(S722F)

    Plasmid
    #32105
    Depositor
    Insert
    Transformation/transcription domain-associated protein (S722F) (TRRAP Human)
    Use
    Tags
    Flag 1x
    Expression
    Mammalian
    Mutation
    Changes serine 722 to Phe
    Promoter
    CMV
    Available since
    Nov. 14, 2011
    Availability
    Academic Institutions and Nonprofits only
  8. R777-E226 Hs.RAPGEF2-nostop

    Plasmid
    #70510
    Purpose
    Gateway ORF clone of human RAPGEF2 [NM_014247.2] without stop codon (for C-terminal fusions)
    Depositor
    Insert
    RAPGEF2 (RAPGEF2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 251 - 300 of 7012 results