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We narrowed to 1,651 results for: Tef

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Showing: 251 - 300 of 1651 results

  5. pCRISPRa_VPR_yl

    Plasmid
    #107677
    Purpose
    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:
    Inserts
    Codon optimized dCas9-VPR
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    107677-plasmid-map-sequence-id-209648

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  6. pRMH120

    Plasmid
    #40593
    Purpose
    Expresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expression
    Depositor
    Chandra Tucker
    Article
    Hughes et al Methods. 2012 Aug 15.
    Inserts
    CRY2 (CRY2 Mustard Weed)
    CIB1 (CIB1 Mustard Weed)
    Expression
    Yeast
    Promoter
    ADH1 and TEF
    Available Since
    Oct. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    40593-plasmid-map-sequence-id-52556

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  7. pAAH1

    Plasmid
    #41825
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Bacterial and Yeast
    Promoter
    T7, TEF
    Available Since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    41825-plasmid-map-sequence-id-56469

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  14. pCRISPRi_Mxi1_yl

    Plasmid
    #91248
    Purpose
    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    91248-plasmid-map-sequence-id-192174

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  26. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid
    #91249
    Purpose
    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    91249-plasmid-map-sequence-id-181276

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  27. pCRISPRyl

    Plasmid
    #70007
    Purpose
    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al ACS Synth Biol. 2015 Dec 29.
    Inserts
    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70007-plasmid-map-sequence-id-137578

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  41. pCOA-Leu-FvPPT1-fsr1

    Plasmid
    #179868
    Purpose
    Constitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1
    Depositor
    Jens Laurids Sørensen
    Article
    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)
    Inserts
    PPT1
    fsr1
    Expression
    Yeast
    Mutation
    Codon optimized for expression in Saccharomyces c…
    Promoter
    Yarrowia lipolytica EXP1 and Yarrowia lipolytica …
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    179868-plasmid-map-sequence-id-352936

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  42. pGG431

    Plasmid
    #165615
    Purpose
    Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165615-plasmid-map-sequence-id-330960

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  43. pJH104

    Plasmid
    #48262
    Purpose
    Template plasmid for PCR-based transplant of Saccharomyces cerevisiae TEF1 promoter in yeast.
    Depositor
    Ronald Davis
    Article
    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10
    Inserts
    TEF1p 5' fragment
    URA3 5' fragment
    TEF1p 3' fragment
    Use
    Routine cloning vector
    Mutation
    From SK1 background. -343 to -1 relative to TEF1 …
    Promoter
    TEF1p
    Available Since
    Feb. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    48262-plasmid-map-sequence-id-69710

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  47. TFORF2693

    Plasmid
    #142359
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TEAD4 (TEAD4 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142359-plasmid-map-sequence-id-397176

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Showing: 251 - 300 of 1651 results