Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 392 results for: SaCas9

Showing: 101 - 120 of 392 results

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nSaCas9 M732 miniABEMax V82G

Plasmid

#135382

Purpose

Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M732 )

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSaCas9 M732 miniABEMax V82G

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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nSaCas9 M741 miniABEMax V82G

Plasmid

#135383

Purpose

Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M741 )

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSaCas9 M741 miniABEMax V82G

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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nSaCas9 N120 miniABEMax V82G

Plasmid

#135384

Purpose

Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N120 )

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSaCas9 N120 miniABEMax V82G

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

Plasmid

#159905

Purpose

Mutagenesis of Slc32a1

Depositor

Larry Zweifel

Insert

Slc32a1 (Slc32a1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgSlc6a3

Plasmid

#159902

Purpose

Mutagenesis of Slc6a3

Depositor

Larry Zweifel

Insert

Slc6a3 (Slc6a3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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AAV CMV-dSaCas9-KRAB-bGHpA

Plasmid

#106219

Purpose

AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgRosa26

Plasmid

#159914

Purpose

Mutagenesis of Rosa26 with SauCas9

Depositor

Larry Zweifel

Insert

Rosa26 gRNA (Gt(ROSA)26Sor Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-CFP-SaCas9-HF (without sgRNA)

Plasmid

#134470

Purpose

Human expression vector for SaCas9-HF variant.

Depositor

Zongli Zheng

Article

Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

Insert

Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity

Use

CRISPR

Tags

SV40NLS-3xFLAG

Expression

Mammalian

Mutation

R245A, N413A, N419A, R654A

Promoter

CAG

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgDrd2

Plasmid

#159918

Purpose

Mutagenesis of Drd2

Depositor

Larry Zweifel

Insert

Drd2 (Drd2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgVamp2

Plasmid

#159920

Purpose

Mutagenesis of Vamp2

Depositor

Larry Zweifel

Insert

Vamp2 (Vamp2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

Plasmid

#159908

Purpose

Mutagenesis of Htr2a

Depositor

Larry Zweifel

Insert

Htr2a (Htr2a Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgTh

Plasmid

#159901

Purpose

Mutagenesis of Th

Depositor

Larry Zweifel

Insert

Th (Th Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgPenk

Plasmid

#159916

Purpose

Mutagenesis of Penk

Depositor

Larry Zweifel

Insert

Penk (Penk Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEXfrt-SaCas9-U6-sgRosa26

Plasmid

#159915

Purpose

Mutagenesis of Rosa26

Depositor

Larry Zweifel

Insert

Rosa26 (Gt(ROSA)26Sor Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-SasgRNA scaffold-CMV-nSaCas9

Plasmid

#171695

Purpose

Expresses nSaCas9 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

sgRNA scaffold-nSaCas9-NLS

Use

CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

U6 and CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnn3

Plasmid

#159904

Purpose

Mutagenesis of Kcnn3

Depositor

Larry Zweifel

Insert

Kcnn3 (Kcnn3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJEP313-pAAV-CMV-SaCas9-DIO-pA

Plasmid

#113690

Purpose

A CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 (NEWENTRY )

Use

AAV, CRISPR, and Cre/Lox

Tags

NLS

Promoter

Cytomegalo Virus(CMV)

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

Plasmid

#209196

Purpose

Mutagenesis of Cnr1

Depositor

Larry Zweifel

Article

Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

Insert

Cnr1 (Cnr1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgDrd1

Plasmid

#159917

Purpose

Mutagenesis of Drd1

Depositor

Larry Zweifel

Insert

Drd1 (Drd1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

Plasmid

#159903

Purpose

Mutagenesis of Oprk1

Depositor

Larry Zweifel

Insert

Oprk1 (Oprk1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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Showing: 101 - 120 of 392 results

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