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Showing: 1181 - 1200 of 1734 results

  1. pCS2+ cMyc-oDam_f_GFPoNLS

    Plasmid
    #85818
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    cMyc oDam_f_GFPoNLS
    Use
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Promoter
    Available since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85818-plasmid-map-sequence-id-169572

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  2. pRD439

    Plasmid
    #168465
    Purpose
    For the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS-mTurquoise2-H-NSdbd-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168465-plasmid-map-sequence-id-331915

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  3. pcDNA4/TO-bio-myc-NES-EGFP

    Plasmid
    #112712
    Purpose
    For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    EGFP
    Use
    Tags
    HIV Rev nuclear localization signal (NES), biotin…
    Expression
    Mammalian
    Mutation
    Nucleotide sequence optimized for synthetic gene …
    Promoter
    CMV with Tet repressor binding sites
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    112712-plasmid-map-sequence-id-217058

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  12. pCMV-lyso-pHoenix

    Plasmid
    #70112
    Purpose
    Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes
    Depositor
    Christian Rosenmund
    Article
    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n
    Insert
    lyso-pHoenix (Cd63 Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse)
    Use
    Tags
    Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    70112-plasmid-map-sequence-id-418643

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  16. pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

    Plasmid
    #190236
    Purpose
    AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase
    Depositor
    Hiroyuki Hioki
    Article
    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi
    Insert
    EGFP
    Use
    AAV
    Tags
    F2A, LDLR C-terminal sequence, mRFP1, myristoylat…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    190236-plasmid-map-sequence-id-381571

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  19. pAAV.hSyn.FLEX.SYFP2-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid
    #105983
    Purpose
    This AAV plasmid in the presence of Cre recombinase expresses an extracellular SYFP2 fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
    Depositor
    Marcel Bruchez
    Article
    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19
    Insert
    FLEX-SYFP2-POST-T2A-dTomato.FLEX
    Use
    AAV and Cre/Lox
    Tags
    SYFP2-POST, T2A-dTomato, and myc
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin 1
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    105983-plasmid-map-sequence-id-235283

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Showing: 1181 - 1200 of 1734 results