165,904 results

1. pHCMV_RVFV_GP

   Plasmid

   #207304

   Purpose

   Mammalian expression of Rift valley fever virus GP protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   RVFV_GP (RVFV_sM_gp1 Rift valley fever virus)
   

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. iRFP-FRB-ER

   Plasmid

   #139317

   Purpose

   endoplasmic reticulum-resident FRB for chemically induced dimerization

   Depositor

   Gerry Hammond

   Article

   Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   iRFP:MTOR(2021-2113):[GGSA]2ILNSRV:SACM1L(521-587)
   

   Expression

   Mammalian

   Available Since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

   Plasmid

   #175571

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-Rab8a-wt

   Plasmid

   #86075

   Purpose

   A mammalian expression plasmid encoding Rab8a wild type with N-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-4T1 SHP2 WT

   Plasmid

   #8322

   Depositor

   Ben Neel

   Article

   O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

   Insert

   SHP2 (PTPN11 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKM446 (Flag-DAS+4))

   Plasmid

   #108321

   Purpose

   Orbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker.

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   attB-Flag-DAS+4
   

   Use

   Orbit integrating plasmid

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)mGAT1-0-GFP

   Plasmid

   #41662

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C-terminal hydrophobic isoleucine residue added a…

   Promoter

   CMV

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A

   Plasmid

   #154773

   Purpose

   plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y125A in G1PylS, hybrid PylT with A41AA and C55A …

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PL-SIN-EF1α-EGFP

   Plasmid

   #21320

   Purpose

   Control Lentiviral vector with strong EF1a promoter, expresses EGFP

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF5B-FRT-cilia-APEX

    Plasmid

    #73186

    Purpose

    Expresses cilia-APEX in mammalian cells

    Depositor

    Maxence Nachury

    Article

    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

    Insert

    cilia-APEX (Nphp3 Mouse, Glycine max)
    

    Tags

    internal GFP tag

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. px459V2.0_Conc2

    Plasmid

    #134451

    Purpose

    Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.4_7C11-1 light chain

    Plasmid

    #198252

    Purpose

    recombinant expression of light chain of monoclonal anti-AMP antibody 7C11-1

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa)
    

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.4_7C11-1 heavy chain

    Plasmid

    #198251

    Purpose

    recombinant expression of heavy chain of monoclonal anti-AMP antibody 7C11-1

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    heavy chain of monoclonal anti-AMP antibody 7C11-1 (mouse IgG2a)
    

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-CCR5

    Plasmid

    #98943

    Purpose

    Mammalian expression plasmid for untagged human CCR5

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST17-UbE2E3

    Plasmid

    #15789

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2E3 (UBE2E3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-Sestrin2 (E451Q)

    Plasmid

    #237420

    Purpose

    Transient expression of N-terminally HA-tagged human Sestrin2 (E451Q)

    Depositor

    David Sabatini

    Article

    Valenstein et al Nature. 2025 Aug 20. doi: 10.1038/s41586

    Insert

    Sestrin2 (SESN2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E451Q

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)

    Plasmid

    #238181

    Purpose

    Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF5 (KLF5 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVeBtA2

    Plasmid

    #238987

    Purpose

    Mammalian expression of bovine arrestin-2 long isoform with Venus N-terminal fusion

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

    Insert

    arrestin-2 long (ARRB2 Bovine)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3VEA3

    Plasmid

    #234796

    Purpose

    Mammalian expression of bovine arrestin-3

    Depositor

    Vsevolod Gurevich

    Article

    Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

    Insert

    Arrestin-3 (ARR3 Bovine)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    none

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMVtrunc F-tractinN9-39-msfGFPΔN7

    Plasmid

    #231552

    Purpose

    Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    F-tractin (Itpka Rat)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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