162,408 results

1. iF53 PB-Zim3-dCas9-mScarlet-Hygro

   Plasmid

   #204726

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Scarlet I-FRET 10-NeonGreen

   Plasmid

   #139364

   Purpose

   FRET Standard

   Depositor

   Richard Day

   Article

   Reissaus et al Biophys J. 2020 Apr 21;118(8):1820-1829.

   Insert

   Scarlet 10AA Neon green
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Autobow

   Plasmid

   #45182

   Depositor

   Joshua Sanes

   Article

   Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

   Insert

   pCAG-Autobow
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRL_U2AF1_S34F_mCherry

   Plasmid

   #84019

   Purpose

   expression of mutant U2AF1 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

   Insert

   U2AF1 (U2AF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   S34F

   Promoter

   PGK

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-myc-Rac1-Q61L

   Plasmid

   #12983

   Depositor

   Gary Bokoch

   Insert

   Rac1 constitutively active (RAC1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Q61L Constitutively active

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6MFV SLC10A1

   Plasmid

   #194130

   Purpose

   Expresses NTCP (SLC10A1) protein in mammalian cells

   Depositor

   Jun Liu

   Insert

   SLC10A1 (SLC10A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHIV-Luciferase

   Plasmid

   #21375

   Purpose

   self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase

   Depositor

   Bryan Welm

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. xCas9(3.7)-ABE(7.10)

   Plasmid

   #108382

   Purpose

   Mammalian expression vector for xCas9(3.7)-ABE

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.7)-ABE(7.10)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEP163

   Plasmid

   #137881

   Purpose

   PiggyBac donor vector for expression of mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   mRFP-T2A-BlasticidinR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EYFP NES visual barcode

    Plasmid

    #158676

    Purpose

    tagging clonal populations with NES EYFP fluorescent protein. Adding NES to EYFP WC

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    EYFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC0056 - LwCas13a-msfGFP-NES

    Plasmid

    #105815

    Purpose

    Expresses active LwaCas13a-NES for mammalian RNA targeting

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a
    

    Use

    CRISPR and Lentiviral

    Tags

    NES and msfGFP-NES-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CL20_mEGFP_ETV6_NTRK3

    Plasmid

    #205817

    Purpose

    Express mEGFP-tagged fusion protein, ETV6_NTRK3 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    ETV6_NTRK3 (ETV6, NTRK3 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTKW106alp7A

    Plasmid

    #69360

    Purpose

    Stabilized lacZ expression vector containing alp7A and hok/sok

    Depositor

    Jeff Hasty

    Article

    Danino et al Sci Transl Med. 2015 May 27;7(289):289ra

    Inserts

    lacZ
    alp7A
    hok
    placIq-lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTac and placIq

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. L4440_BioBrick-hsf-1-sgRNA-A

    Plasmid

    #177786

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    hsf-1 (hsf-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLZts

    Plasmid

    #128799

    Purpose

    Temperature-sensitive vector for allelic replacement mutagenesis of Group A Streptococcus

    Depositor

    Timothy Barnett

    Article

    Barnett et al Methods Mol Biol. 2020;2136:59-69. doi:

    Type

    Empty backbone

    Use

    Bacterial mutagenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC-sleeping beauty

    Plasmid

    #65487

    Purpose

    HSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.

    Depositor

    Guillaume Filion

    Insert

    sleeping beauty transposase
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    HSP

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223-ACVR2B

    Plasmid

    #23401

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ACVR2B (ACVR2B Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    A333G, 1MM

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

18. Ai162 (TIT2L-GC6s-ICL-tTA2) targeting vector

    Plasmid

    #114433

    Purpose

    Target a Cre-dependent GCaMP6s cassette and a tTA2 cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    GCaMP6s, tTA2
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    TRE2, CAG

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEXfrt-SaCas9-U6-sgRNA

    Plasmid

    #124845

    Purpose

    Vector for FLP-dependent expression of SaCas9 with gRNA

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SauCas9 gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_Lifeact-mCherry

    Plasmid

    #67302

    Purpose

    Fluorescent indicator for F-actin

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

    Insert

    Lifeact-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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