159,617 results

1. pBEVY-GL

   Plasmid

   #51225

   Purpose

   bi-directional expression vector for yeast, galactose inducible, LEU2 selection

   Depositor

   Charles Miller

   Article

   Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1,10

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTECH-MmAcK3RS(IPYE)

   Plasmid

   #104071

   Purpose

   Expression plasmid encoding MmAcK3RS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp MmAcK3RS(IPYE); PproK pyl
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHis-BRD4 BD1

   Plasmid

   #196544

   Purpose

   Expression of BRD4 Bromodomain (BD1) in E.coli

   Depositor

   Byung Il Lee

   Article

   Kim et al Sci Rep. 2023 Jul 4;13(1):10805. doi: 10

   Insert

   BRD4 BD1 (BRD4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEntry_SARS-CoV-2-Nucleocapsid

   Plasmid

   #168886

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   SARS-CoV-2-Nucleocapsid (N )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-minCMV-mCherry

   Plasmid

   #27970

   Depositor

   Feng Zhang

   Article

   Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pITGA4-GFP

   Plasmid

   #81178

   Purpose

   Mammalian expression of human full length ITGA4-EGFP

   Depositor

   Chinten James Lim

   Insert

   ITGA4 (ITGA4 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains KLGFFKR motif; A1002L (please see deposi…

   Promoter

   CMV

   Available since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-SKP2

   Plasmid

   #215092

   Purpose

   Expresses FLAG-SKP2 in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   SKP2 (SKP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFBOH-LIC

   Plasmid

   #26099

   Purpose

   SGC Empty baculovirus expression/transfer vector

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   6x His and Thrombin protease cleavage site

   Expression

   Mutation

   Promoter

   polyhedrin

   Available since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. GB-NES

   Plasmid

   #61017

   Purpose

   ddGFP B-NES

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP B
   

   Use

   Tags

   HindIII site and NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSC168

    Plasmid

    #216350

    Purpose

    xhNup35-Nb1t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup35-Nb1t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PA-mCherry1-H2B-C-10

    Plasmid

    #57181

    Purpose

    Localization: Histones/Nucleus, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    PA-mCherry1

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKH116_keima-YIPF3

    Plasmid

    #214970

    Purpose

    Golgiphagy flux reporter keima-YIPF3

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    keima-YIPF3 (YIPF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB PUb-integrase

    Plasmid

    #183966

    Purpose

    piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    PhiC31 integrase
    

    Use

    Piggybac insect transgenesis destination vector

    Tags

    Expression

    Mutation

    Promoter

    Aedes aegypti Polyubiquitin

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBMN FKBP(DD)-YFP

    Plasmid

    #31763

    Depositor

    Thomas Wandless

    Article

    Banaszynski et al Cell. 2006 Sep 8;126(5):995-1004.

    Insert

    FKBP(DD)-YFP-HA (FKBP1A Human)
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    F36V, L106P on FKBP12

    Promoter

    LTR

    Available since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGoldenGreenGate-M (pGGG-M)

    Plasmid

    #165422

    Purpose

    A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).

    Depositor

    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Level 2 golden gate (moclo) du…

    Tags

    Expression

    Mutation

    Promoter

    Contain GG cassette and MCS with LacZ for blue/wh…

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

    Plasmid

    #71294

    Purpose

    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Use

    Tags

    GNSTM glycosylation motif, HA, Lamp2 signal pepti…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLPT234

    Plasmid

    #127855

    Purpose

    Repressilator 2.0, without degradation tags, integrated triple reporter

    Depositor

    Johan Paulsson

    Article

    Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:

    Inserts

    bla
    mSCFP3
    mKate2
    mVenus
    TetR
    LacI
    cI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    First 11aa derived from mCherry as fusion protein

    Promoter

    pAmpR, pL LacO1, pL tetO1, and pR

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC2A2

    Plasmid

    #132126

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC2A2 (SLC2A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. MS2-Snapf

    Plasmid

    #65860

    Purpose

    Expresses RNA-binding protein MS2 with fast SNAP fusion tag

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

    Inserts

    MS2
    Fast SNAP
    

    Use

    Tags

    HA and NLS

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

20. G13 sensor (Ga13_2)

    Plasmid

    #112930

    Purpose

    A FRET sensor for G13 activation, encoded on a single plasmid.

    Depositor

    Dorus Gadella

    Article

    Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

    Insert

    G13 sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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