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TDP-43_NTD1-80_Y4R

Plasmid

#104478

Purpose

bacterial expression of TDP-43 NTD Y4R

Depositor

Nicolas Fawzi

Article

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Tags

His

Expression

Bacterial

Mutation

Y4R

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCR4-Dact1(1250-1601)

Plasmid

#52046

Purpose

To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.

Depositor

Ben Cheyette

Article

Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.

Insert

Dact1(1250-1601) (Dact1 Mouse)

Expression

Bacterial

Available Since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-p19Ink4d-IRES-GFP

Plasmid

#52221

Purpose

retroviral bicistronic expression of p19Ink4d and GFP

Depositor

Martine Roussel

Article

Forget et al Cell Cycle. 2008 Dec;7(23):3737-46. Epub

Insert

Ink4d (Cdkn2d Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA HA-Gnat1 WT-3UTR

Plasmid

#184026

Purpose

Expresses mouse Gnat1 protein with N-terminal HA tag from cDNA that contains the full length 3'-UTR

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Gnat1 (Gnat1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2

Plasmid

#193351

Purpose

Retroviral vector expressing human SOX2

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX

Plasmid

#163571

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-NG-nCas9-RBMX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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mCardinal-H3-23

Plasmid

#56163

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H3 (H3C2 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pPB-CAG-Klf4-pA-pgk-hph

Plasmid

#74906

Purpose

Mammalian expression of mKlf4

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Insert

Klf4 (Klf4 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

Plasmid

#91882

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Suz12 (Suz12 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
Cas9_YTHDF2_sgRNA

Plasmid

#186673

Purpose

YTHDF2 sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/T0-GFP-BRCA1 1364-1437

Plasmid

#71119

Purpose

Mammalian expression of GFP-BRCA1 1364-1437

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 1364-1437 of BRCA1

Promoter

CMV/TO

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-Gluc-EL222-his

Plasmid

#172097

Purpose

Expresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteria

Depositor

Avi Schroeder

Article

Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

Insert

Gaussia Luciferase - EL222

Expression

Bacterial

Mutation

Gluc mutations - M43L, M110L

Promoter

T7

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97310

Purpose

NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb NHEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_GPR160

Plasmid

#45274

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

GPR160 (GPR160 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pCMV_RBMX-eA3A-UdgX-NG-nCas9

Plasmid

#163551

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-GOLPH3

Plasmid

#21688

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Gateway entry clone

Mutation

last nucleotide of stop codon removed for c-termi…

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
Hook3(1-522)-SNAPf-Psc-StrepII

Plasmid

#111860

Purpose

C-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

Hook3(1-552)-SNAPf (HOOK3 Human)

Tags

Psc, SNAPf, and Strep-II

Expression

Insect

Mutation

Optimised for expression in Sf9 cells

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMX-IY LC3B

Plasmid

#89291

Purpose

For expression of the mammalian Atg8 protein LC3B without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

LC3B (MAP1LC3B Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pWzl_neo_DEST_flag_SOX2

Plasmid

#45309

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

MCOPS3 (SOX2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
mCardinal-Alpha-Actinin-19

Plasmid

#56152

Purpose

Localization: Focal Adhesions / Actin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits